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For this reason, a new coprological loop-mediated isothermal amplification (LAMP) assay was developed for the simultaneous detection of these species. The primer set was designed on a region of the mitochondrial <jats:italic>cox<\/jats:italic>-1 gene. Amplification conditions were evaluated using a temperature gradient (52\u00b0C to 68\u00b0C), different incubation times (15\u2013120 min), and different concentrations of malachite green dye (0.004\u20130.4% w\/v). The analytical sensitivity was evaluated with serial dilutions of genomic DNA from <jats:italic>T. canis<\/jats:italic> and <jats:italic>T. cati<\/jats:italic> adult worms, and with serial dilutions of DNA extracted from feces using a low-cost in-house method. The specificity was evaluated using genomic DNA from <jats:italic>Canis lupus familiaris<\/jats:italic>, <jats:italic>Felis catus<\/jats:italic>, <jats:italic>Escherichia coli<\/jats:italic>, <jats:italic>Toxascaris leonina<\/jats:italic>, <jats:italic>Ancylostoma caninum<\/jats:italic>, <jats:italic>Echinococcus granulosus sensu stricto<\/jats:italic> and <jats:italic>Taenia hydatigena<\/jats:italic>. The LAMP assay applied to environmental fecal samples from an endemic area showed an analytical sensitivity of 10\u2013100 fg of genomic DNA and 10<jats:sup>\u22125<\/jats:sup> serial dilutions of DNA extracted from feces using the low-cost in-house method; with a specificity of 100%. Additionally, the total development of the assay was carried out in a basic laboratory and per-reaction reagent cost decreased by ~80%. This new, low-cost tool can help identify the most common agents of toxocariasis in endemic areas in order to manage prevention strategies without having to rely on a laboratory with sophisticated equipment.<\/jats:p>","DOI":"10.1017\/s0031182021000342","type":"journal-article","created":{"date-parts":[[2021,2,17]],"date-time":"2021-02-17T06:21:30Z","timestamp":1613542890000},"page":"819-826","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Development of a low-cost copro-LAMP assay for simultaneous copro-detection of <i>Toxocara canis<\/i> and <i>Toxocara cati<\/i>"],"prefix":"10.1017","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9479-4117","authenticated-orcid":false,"given":"H\u00e9ctor Gabriel","family":"Avila","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marikena Guadalupe","family":"Risso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Ruybal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Anal\u00eda","family":"Repetto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos Javier","family":"Butti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos David","family":"Trangoni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylvia","family":"Grune L\u00f6ffler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica Mirtha","family":"P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Victoria","family":"Periago","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2021,2,17]]},"reference":[{"key":"S0031182021000342_ref64","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(00)00323-X"},{"key":"S0031182021000342_ref65","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-018-5843-5"},{"key":"S0031182021000342_ref17","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-288"},{"key":"S0031182021000342_ref1","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822014000400032"},{"key":"S0031182021000342_ref30","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-017-5553-4"},{"key":"S0031182021000342_ref53","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.02.003"},{"key":"S0031182021000342_ref40","doi-asserted-by":"publisher","DOI":"10.3390\/ani9100827"},{"key":"S0031182021000342_ref49","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2014.450"},{"key":"S0031182021000342_ref11","first-page":"5","article-title":"Toxocariasis, intestinal parasitoses and eosinophilia in school children from Argentina","volume":"42","author":"Ciarmela","year":"2016","journal-title":"Current Health Sciences Journal"},{"key":"S0031182021000342_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2013.12.016"},{"key":"S0031182021000342_ref66","doi-asserted-by":"publisher","DOI":"10.3390\/molecules201219787"},{"key":"S0031182021000342_ref41","first-page":"503","article-title":"Hair coat contamination with zoonotic helminth eggs of farm and pet dogs and foxes","volume":"124","author":"Nagy","year":"2011","journal-title":"Berliner Und Munchener Tierarztliche Wochenschrift"},{"key":"S0031182021000342_ref52","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.057901"},{"key":"S0031182021000342_ref4","unstructured":"Avila, HG (2019) Echinococcus granulosus sensu lato en argentina: obtenci\u00f3n de mapa epidemiol\u00f3gico y desarrollo de un sistema de diagn\u00f3stico molecular aplicable en programas de control de la hidatidosis (PhD thesis). 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