{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T07:31:52Z","timestamp":1790753512260,"version":"4.1.0"},"reference-count":65,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T00:00:00Z","timestamp":1615161600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The large-sized tapeworm <jats:italic>Dibothriocephalus latus<\/jats:italic> is known as the broad or fish-borne cestode of mammals that is capable to infect humans and cause diphyllobothriosis. Recently, molecular data on <jats:italic>D. latus<\/jats:italic> has been accumulating in the literature and a complete genome sequence has been published; however, little is known about the karyotype and chromosome architecture. In this study, an in-depth karyological analysis of 2 <jats:italic>D. latus<\/jats:italic> specimens was carried out. The plerocercoids originated from a perch caught in subalpine Lake Iseo (Italy) and the tapeworms were reared in hamsters. Both specimens contained cells with a highly variable number of chromosomes ranging from18 to 27. Nevertheless, the largest portion of mitotic figures (47%) showed a number corresponding to the triploid set, 3<jats:italic>n<\/jats:italic> = 27. Accordingly, the karyotype of the analyzed specimens consisted of 9 triplets of metacentric chromosomes. Fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) with the 18S rDNA probe clearly demonstrated the presence of 3 clusters of hybridization signals on the triplet of chromosome 7, thus confirming the triploid status of the specimens. FISH with a telomeric (TTAGGG)<jats:italic><jats:sub>n<\/jats:sub><\/jats:italic> probe confined hybridization signals exclusively to the terminal chromosomal regions, supporting the earlier findings that this repetitive motif is a conserved feature of tapeworm telomeres.<\/jats:p>","DOI":"10.1017\/s0031182021000408","type":"journal-article","created":{"date-parts":[[2021,3,8]],"date-time":"2021-03-08T05:13:34Z","timestamp":1615180414000},"page":"787-797","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":12,"title":["Molecular cytogenetic analysis of a triploid population of the human broad tapeworm, <i>Dibothriocephalus latus<\/i> (Diphyllobothriidea)"],"prefix":"10.1017","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8934-9126","authenticated-orcid":false,"given":"Martina","family":"Orosov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Markov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2336-458X","authenticated-orcid":false,"given":"Irena","family":"Provazn\u00edkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0518-5708","authenticated-orcid":false,"given":"Mikul\u00e1\u0161","family":"Oros","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Al\u017ebeta","family":"Rada\u010dovsk\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"\u010cadkov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6745-5603","authenticated-orcid":false,"given":"Franti\u0161ek","family":"Marec","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2021,3,8]]},"reference":[{"key":"S0031182021000408_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2018.08.003"},{"key":"S0031182021000408_ref18","doi-asserted-by":"publisher","DOI":"10.1508\/cytologia.66.39"},{"key":"S0031182021000408_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.022"},{"key":"S0031182021000408_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2019.10.039"},{"key":"S0031182021000408_ref16","doi-asserted-by":"publisher","DOI":"10.2307\/3280954"},{"key":"S0031182021000408_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000047818"},{"key":"S0031182021000408_ref56","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1276"},{"key":"S0031182021000408_ref27","first-page":"299","volume-title":"Biology 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