{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T21:20:14Z","timestamp":1785878414877,"version":"3.56.0"},"reference-count":49,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000383","name":"Higher Education Funding Council for Wales","doi-asserted-by":"publisher","award":["Enhancing HE-FE Collaboration in Innovation and En"],"award-info":[{"award-number":["Enhancing HE-FE Collaboration in Innovation and En"]}],"id":[{"id":"10.13039\/501100000383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Environmental DNA (eDNA) surveying has potential to become a powerful tool for sustainable parasite control. As trematode parasites require an intermediate snail host that is often aquatic or amphibious to fulfil their lifecycle, water-based eDNA analyses can be used to screen habitats for the presence of snail hosts and identify trematode infection risk areas. The aim of this study was to identify climatic and environmental factors associated with the detection of <jats:italic>Galba truncatula<\/jats:italic> eDNA. Fourteen potential <jats:italic>G. truncatula<\/jats:italic> habitats on two farms were surveyed over a 9-month period, with eDNA detected using a filter capture, extraction and PCR protocol with data analysed using a generalized estimation equation. The probability of detecting <jats:italic>G. truncatula<\/jats:italic> eDNA increased in habitats where snails were visually detected, as temperature increased, and as water pH decreased (<jats:italic>P<\/jats:italic> &lt; 0.05). Rainfall was positively associated with eDNA detection in watercourse habitats on farm A, but negatively associated with eDNA detection in watercourse habitats on farm B (<jats:italic>P<\/jats:italic> &lt; 0.001), which may be explained by differences in watercourse gradient. This study is the first to identify factors associated with trematode intermediate snail host eDNA detection. These factors should be considered in standardized protocols to evaluate the results of future eDNA surveys.<\/jats:p>","DOI":"10.1017\/s0031182021001104","type":"journal-article","created":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T04:23:16Z","timestamp":1625026996000},"page":"1490-1496","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":12,"title":["Temporal dynamics of trematode intermediate snail host environmental DNA in small water body habitats"],"prefix":"10.1017","volume":"148","author":[{"given":"Rhys Aled","family":"Jones","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2730-4982","authenticated-orcid":false,"given":"Chelsea N.","family":"Davis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dewi Llyr","family":"Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Tyson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emma","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Cutress","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Brophy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Rose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manod","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hefin Wyn","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2021,6,30]]},"reference":[{"key":"S0031182021001104_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.03.002"},{"key":"S0031182021001104_ref45","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.019"},{"key":"S0031182021001104_ref34","doi-asserted-by":"publisher","DOI":"10.1111\/1365-2664.12306"},{"key":"S0031182021001104_ref17","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2019.1409"},{"key":"S0031182021001104_ref41","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32680-z"},{"key":"S0031182021001104_ref40","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.04743"},{"key":"S0031182021001104_ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224617"},{"key":"S0031182021001104_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12682"},{"key":"S0031182021001104_ref4","volume-title":"Model Section and Multimodel Inferences: A Practical-Theoretic Approach","author":"Burnham","year":"2002"},{"key":"S0031182021001104_ref29","doi-asserted-by":"publisher","DOI":"10.1002\/edn3.53"},{"key":"S0031182021001104_ref39","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-017-0005-3"},{"key":"S0031182021001104_ref24","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-1271-x"},{"key":"S0031182021001104_ref37","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12052"},{"key":"S0031182021001104_ref20","doi-asserted-by":"publisher","DOI":"10.1890\/14-1530.1"},{"key":"S0031182021001104_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.01.008"},{"key":"S0031182021001104_ref14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016126"},{"key":"S0031182021001104_ref27","doi-asserted-by":"publisher","DOI":"10.1086\/699203"},{"key":"S0031182021001104_ref9","volume-title":"The Mud Snail (Galba truncatula) Ecology, Parasitism and Control","author":"Dreyfuss","year":"2015"},{"key":"S0031182021001104_ref48","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2015.12.023"},{"key":"S0031182021001104_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.017"},{"key":"S0031182021001104_ref26","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-018-2928-z"},{"key":"S0031182021001104_ref42","doi-asserted-by":"publisher","DOI":"10.1007\/s10531-019-01709-8"},{"key":"S0031182021001104_ref21","volume-title":"IBM SPSS Statistics for Windows, Version 27.0","year":"2020"},{"key":"S0031182021001104_ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2018.06.018"},{"key":"S0031182021001104_ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.0006-341X.2001.00120.x"},{"key":"S0031182021001104_ref35","first-page":"57","article-title":"Les r\u00e9seaux de drainage superficiel et leur colonisation par Lymnaea truncatula M\u00fcller. A propos de quatre ann\u00e9es d'observations en Haute-Vienne, France","volume":"14","author":"Rondelaud","year":"1983","journal-title":"Annales de Recherches V\u00e9t\u00e9rinaires"},{"key":"S0031182021001104_ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2021.109373"},{"key":"S0031182021001104_ref49","doi-asserted-by":"publisher","DOI":"10.1641\/0006-3568(2001)051[0537:FIAE]2.0.CO;2"},{"key":"S0031182021001104_ref5","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-014-0528-0"},{"key":"S0031182021001104_ref8","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms12544"},{"key":"S0031182021001104_ref31","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28424-8"},{"key":"S0031182021001104_ref15","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b06829"},{"key":"S0031182021001104_ref23","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4802"},{"key":"S0031182021001104_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12508"},{"key":"S0031182021001104_ref16","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12595"},{"key":"S0031182021001104_ref22","doi-asserted-by":"publisher","DOI":"10.1111\/1755-0998.12285"},{"key":"S0031182021001104_ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2014.11.038"},{"key":"S0031182021001104_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2015.06.013"},{"key":"S0031182021001104_ref7","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-020-04371-0"},{"key":"S0031182021001104_ref10","doi-asserted-by":"publisher","DOI":"10.1051\/limn\/2018010"},{"key":"S0031182021001104_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2017.03.021"},{"key":"S0031182021001104_ref38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1815046116"},{"key":"S0031182021001104_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/BF00380403"},{"key":"S0031182021001104_ref6","doi-asserted-by":"publisher","DOI":"10.1177\/1536867X0700700205"},{"key":"S0031182021001104_ref11","volume-title":"A Decade of EU-Funded Animal Health Research","year":"2012"},{"key":"S0031182021001104_ref44","doi-asserted-by":"publisher","DOI":"10.1111\/2041-210X.12994"},{"key":"S0031182021001104_ref47","volume-title":"Veterinary Parasitology","author":"Urquhart","year":"1996"},{"key":"S0031182021001104_ref36","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1283-5"},{"key":"S0031182021001104_ref3","volume-title":"The Nature and Properties of Soils","author":"Brady","year":"2002"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182021001104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,29]],"date-time":"2023-05-29T09:55:10Z","timestamp":1685354110000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182021001104\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,30]]},"references-count":49,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["S0031182021001104"],"URL":"https:\/\/doi.org\/10.1017\/s0031182021001104","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,30]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2021. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}