{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T06:45:19Z","timestamp":1776408319022,"version":"3.51.2"},"reference-count":33,"publisher":"Cambridge University Press (CUP)","issue":"12","license":[{"start":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T00:00:00Z","timestamp":1625443200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Immune complexes (ICs) are found in canine visceral leishmaniasis (CVL) and interfere with the serum detection of antibodies. Dissociation of these monovalent complexes by dissociative enzyme-linked immunosorbent assay (ELISA) removes false-negative results and allows some characterization of antibodies and antigens. We studied the serology of dogs with suspected CVL in an endemic area, testing two <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Leishmania<\/jats:italic>) [<jats:italic>L.<\/jats:italic> (<jats:italic>L.<\/jats:italic>)] <jats:italic>&lt;full form&gt;infantum<\/jats:italic> antigens. We analysed the presence of immunoglobulin G (IgG), immunoglobulin A (IgA) and immunoglobulin M (IgM) antibodies specific to promastigote soluble extract (PSE) and low-molecular weight glycans (glycan\u2013bovine serum albumin (BSA) complex \u2013 GBC) by conventional and dissociative ELISA. Our results showed a significant fraction of IgA ICs (46.5% for PSE and 47.6% for GBC), followed by IgG ICs (10% for PSE and 23.5% for GBC). IgM ICs were more frequent for PSE (22.7%). Hypergammaglobulinaemia in CVL would be related to the presence of IgA and IgG ICs, resulting in deficient elimination of these antibodies. Our data confirmed the presence of ICs that can generate false-negative results in conventional serology. The production of IgA antibodies and the high frequency of blockade by glycan antigens suggest the active participation of this immunoglobulin and its ICs in the immunopathology of CVL, indicating a new path for further research.<\/jats:p>","DOI":"10.1017\/s0031182021001189","type":"journal-article","created":{"date-parts":[[2021,7,5]],"date-time":"2021-07-05T03:05:51Z","timestamp":1625454351000},"page":"1509-1515","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Serum antibodies blocked by glycan antigens in canine visceral leishmaniasis serology are mostly IgA immune complexes"],"prefix":"10.1017","volume":"148","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1762-2749","authenticated-orcid":false,"given":"Camila","family":"Aparecida de Carvalho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Mitsuyoshi Hiramoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luciana","family":"Regina Meireles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4697-4647","authenticated-orcid":false,"given":"Heitor","family":"Franco de Andrade J\u00fanior","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2021,7,5]]},"reference":[{"key":"S0031182021001189_ref15","doi-asserted-by":"publisher","DOI":"10.1038\/nri3582"},{"key":"S0031182021001189_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2019.01.022"},{"key":"S0031182021001189_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.03.032"},{"key":"S0031182021001189_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2019.06.002"},{"key":"S0031182021001189_ref31","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182019001355"},{"key":"S0031182021001189_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2019.11.001"},{"key":"S0031182021001189_ref12","first-page":"24","article-title":"Animal reservoirs for visceral leishmaniasis in densely populated urban areas","volume":"2","author":"Diniz","year":"2008","journal-title":"Journal of Infection in Developing Countries"},{"key":"S0031182021001189_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.108921"},{"key":"S0031182021001189_ref10","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06851-9"},{"key":"S0031182021001189_ref16","doi-asserted-by":"publisher","DOI":"10.1017\/S003118201600055X"},{"key":"S0031182021001189_ref6","doi-asserted-by":"publisher","DOI":"10.1039\/C5OB00563A"},{"key":"S0031182021001189_ref14","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822012000100006"},{"key":"S0031182021001189_ref9","doi-asserted-by":"publisher","DOI":"10.1111\/imm.13118"},{"key":"S0031182021001189_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2006.02.001"},{"key":"S0031182021001189_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2011.12.009"},{"key":"S0031182021001189_ref19","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00302"},{"key":"S0031182021001189_ref29","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.581148"},{"key":"S0031182021001189_ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-017-3699-z"},{"key":"S0031182021001189_ref18","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00055"},{"key":"S0031182021001189_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2020.109276"},{"key":"S0031182021001189_ref25","doi-asserted-by":"publisher","DOI":"10.1556\/1886.2020.00018"},{"key":"S0031182021001189_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2020.02.004"},{"key":"S0031182021001189_ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2008.04.001"},{"key":"S0031182021001189_ref5","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00245"},{"key":"S0031182021001189_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-020-06845-7"},{"key":"S0031182021001189_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.10.010"},{"key":"S0031182021001189_ref23","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75569-6"},{"key":"S0031182021001189_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(99)00048-3"},{"key":"S0031182021001189_ref2","doi-asserted-by":"publisher","DOI":"10.1373\/jalm.2018.028282"},{"key":"S0031182021001189_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2007.01.015"},{"key":"S0031182021001189_ref22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119298"},{"key":"S0031182021001189_ref17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192302"},{"key":"S0031182021001189_ref32","unstructured":"World Health Organization (2019) Leishmaniasis. 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