{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T13:58:51Z","timestamp":1784815131254,"version":"3.55.0"},"reference-count":32,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Grant-in-Aid for Challenging Research","award":["20K21357"],"award-info":[{"award-number":["20K21357"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["B: 18H02332"],"award-info":[{"award-number":["B: 18H02332"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["B: 20H03137"],"award-info":[{"award-number":["B: 20H03137"]}]},{"name":"Grant-in-Aid for the Japan Society for the Promotion of Science Research Fellow","award":["20J22235"],"award-info":[{"award-number":["20J22235"]}]},{"name":"Grant-in-Aid for Challenging Research","award":["20K21357"],"award-info":[{"award-number":["20K21357"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["B: 18H02332"],"award-info":[{"award-number":["B: 18H02332"]}]},{"name":"Grant-in-Aid for Scientific Research","award":["B: 20H03137"],"award-info":[{"award-number":["B: 20H03137"]}]},{"name":"Grant-in-Aid for the Japan Society for the Promotion of Science Research Fellow","award":["20J22235"],"award-info":[{"award-number":["20J22235"]}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Poultry red mites (<jats:italic>Dermanyssus gallinae<\/jats:italic>, PRM) are dangerous ectoparasites that infest chickens and threaten the poultry industry worldwide. PRMs usually develop resistance to chemical acaricides, necessitating the development of more effective preventive agents, and vaccination could be an alternative strategy for controlling PRMs. The suitability of plasma membrane proteins expressed in the midguts as vaccine antigens was evaluated because these molecules are exposed to antibodies in the ingested blood and the binding of antibodies could potentially induce direct damage to midgut tissue and indirect damage <jats:italic>via<\/jats:italic> inhibition of the functions of target molecules. Therefore, in the present study, a copper transporter 1-like molecule (Dg-Ctr1) was identified and its efficacy as a vaccine antigen was assessed <jats:italic>in vitro<\/jats:italic>. <jats:italic>Dg-Ctr1<\/jats:italic> mRNA was expressed in the midguts and ovaries and in all the life stages, and flow cytometric analysis indicated that Dg-Ctr1 was expressed on the plasma membrane. Importantly, nymphs fed on plasma derived from chickens immunized with the recombinant protein of the extracellular region of Dg-Ctr1 showed a significant reduction in the survival rate. These data indicate that the application of Dg-Ctr1 as a vaccine antigen could reduce the number of nymphs in the farms, contributing to reduction in the economic losses caused by PRMs in the poultry industry. To establish an effective vaccination strategy, the acaricidal effects of the combined use of Dg-Ctr1 with chemical acaricides or other vaccine antigens must be examined.<\/jats:p>","DOI":"10.1017\/s0031182021001608","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T07:30:36Z","timestamp":1631259036000},"page":"105-115","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Characterization of a copper transporter 1 from <i>Dermanyssus gallinae<\/i> as a vaccine antigen"],"prefix":"10.1017","volume":"149","author":[{"given":"Sotaro","family":"Fujisawa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2569-899X","authenticated-orcid":false,"given":"Shiro","family":"Murata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Isezaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuma","family":"Ariizumi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Oishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Taneno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Maekawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohiro","family":"Okagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Ichii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Konnai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiko","family":"Ohashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"key":"S0031182021001608_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011002356"},{"key":"S0031182021001608_ref3","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/165812"},{"key":"S0031182021001608_ref21","doi-asserted-by":"publisher","DOI":"10.1080\/03079457.2015.1030589"},{"key":"S0031182021001608_ref30","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.4049\/jimmunol.143.4.1346","article-title":"Immunologic control of a parasitic arthropod. 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