{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:55:15Z","timestamp":1787100915803,"version":"build-2736575974"},"reference-count":38,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"name":"Thai Government Research Fund through Thammasat University","award":["50\/2559 and 64\/2560"],"award-info":[{"award-number":["50\/2559 and 64\/2560"]}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human liver fluke <jats:italic>Opisthorchis viverrini<\/jats:italic> (<jats:italic>Ov<\/jats:italic>), the primary risk factor for cholangiocarcinoma (CHCA), is a parasite endemic to southeast Asian countries. With no effective treatments for CHCA currently available, early diagnosis and treatment of <jats:italic>Ov<\/jats:italic> infection remains the only practical method for the prevention of CHCA. In this study, plasma phosphoproteomes of patients in the non-<jats:italic>Ov<\/jats:italic> infection, non-cholangiocarcinoma subject group (non-OVCCA), the asymptomatic <jats:italic>Ov<\/jats:italic> infected group (OV), and the CHCA group (CCA), were investigated to identify potential biomarkers for <jats:italic>Ov<\/jats:italic> infection and CHCA. The AKT signalling pathway was found to be up-regulated. Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta isoform (PIK3CB), an upstream signalling molecule, was selected as a potential biomarker and evaluated using indirect enzyme-linked immunosorbent assay (ELISA). Results demonstrated evidence that levels of PIK3CB in both the OV group and CCA group was statistically different compared to the non-OVCCA group (<jats:italic>P<\/jats:italic> &lt; 0.01). However, the levels of PIK3CB between the OV group and the CCA group were found not to be statistically different. Sensitivity and specificity for OV using OD450 cut-off at &gt;1.570 was 76 and 72%, respectively. For CCA, sensitivity and specificity using OD450 cut-off at &gt;1.398 was 68 and 76%, respectively. Application of indirect ELISA detecting plasma PIK3CB will be of great benefit for screening of opisthorchiasis and CHCA.<\/jats:p>","DOI":"10.1017\/s0031182021001694","type":"journal-article","created":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T02:27:10Z","timestamp":1633400830000},"page":"171-180","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit beta as a potential biomarker for <i>Opisthorchis viverrini<\/i> infection and 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