{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T20:46:27Z","timestamp":1772311587166,"version":"3.50.1"},"reference-count":35,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T00:00:00Z","timestamp":1636070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Assays which enable the detection of schistosome gut-associated circulating anodic (CAA) and cathodic (CCA) antigen in serum or urine are increasingly used as a diagnostic tool for schistosome infection. However, little is known about the production and clearance of these circulating antigens in relation to the sex and reproductive maturity of the parasite. Here we describe CAA and CCA excretion patterns by exploring a mouse model after exposure to 36 male-only, female-only and mixed (male\/female) <jats:italic>Schistosoma mansoni<\/jats:italic> cercariae. We found that serum and urine CAA levels, analysed at 3 weeks intervals, peaked at 6 weeks post-infection. Worms recovered after perfusion at 14 weeks were cultured <jats:italic>ex vivo<\/jats:italic>. Male parasites excreted more circulating antigens than females, in the mouse model as well as <jats:italic>ex vivo<\/jats:italic>. In mixed infections (supporting egg production), serum CAA levels correlated to the number of recovered worms, whereas faecal egg counts or <jats:italic>Schistosoma<\/jats:italic> DNA in stool did not. No viable eggs and no inflammation were seen in the livers from mice infected with female worms only. <jats:italic>Ex vivo<\/jats:italic>, CAA levels were higher than CCA levels. Our study confirms that CAA levels reflect worm burden and allows detection of low-level single-sex infections.<\/jats:p>","DOI":"10.1017\/s0031182021001839","type":"journal-article","created":{"date-parts":[[2021,11,5]],"date-time":"2021-11-05T03:13:28Z","timestamp":1636082008000},"page":"306-313","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":11,"title":["Excretion patterns of <i>Schistosoma mansoni<\/i> antigens CCA and CAA by adult male and female worms, using a mouse model and <i>ex vivo<\/i> parasite cultures"],"prefix":"10.1017","volume":"149","author":[{"given":"Miriam","family":"Casacuberta-Partal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisette","family":"van Lieshout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"van 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