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Still, specimens associated with a particular DNA sequence must be identified by the use of traditional morphology-based analysis and correct linking of sequence and identification must be ensured. Only a small part of DNA sequences of the genus <jats:italic>Diplostomum<\/jats:italic> (Diplostomidae) is based on adult isolates which are essential for accurate identification. In this study, we provide species identification with an aid of morphological and molecular (<jats:italic>cox<\/jats:italic>1, ITS-5.8S-ITS2 and 28S) characterization of adults of <jats:italic>Diplostomum baeri<\/jats:italic> Dubois, 1937 from naturally infected <jats:italic>Larus canus<\/jats:italic> Linnaeus in Karelia, Russia. Furthermore, we reveal that the DNA sequences of our isolates of <jats:italic>D. baeri<\/jats:italic> are identical with those of the lineage <jats:italic>Diplostomum<\/jats:italic> sp. clade Q , while other sequences labelled as the \u2018<jats:italic>D. baeri<\/jats:italic>\u2019 complex do not represent lineages of <jats:italic>D. baeri<\/jats:italic>. Our new material of cercariae from <jats:italic>Radix balthica<\/jats:italic> (Linnaeus) in Ireland is also linked to <jats:italic>Diplostomum<\/jats:italic> sp. clade Q. We reveal that <jats:italic>D. baeri<\/jats:italic> is widely distributed in Europe; as first intermediate hosts lymnaeid snails (<jats:italic>Radix auricularia<\/jats:italic> (Linnaeus), <jats:italic>R. balthica<\/jats:italic>) are used; metacercariae occur in eye lens of cyprinid fishes. In light of the convoluted taxonomy of <jats:italic>D. baeri<\/jats:italic> and other <jats:italic>Diplostomum<\/jats:italic> spp., we extend the recommendations of Blasco-Costa <jats:italic>et al<\/jats:italic>. (2016, Systematic Parasitology <jats:bold>93<\/jats:bold>, 295\u2013306) for the \u2018best practice\u2019 in molecular approaches to trematode systematics. The current study is another step in elucidating the species spectrum of <jats:italic>Diplostomum<\/jats:italic> based on integrative taxonomy with well-described morphology of adults linked to sequences.<\/jats:p>","DOI":"10.1017\/s0031182021002092","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T08:27:40Z","timestamp":1641544060000},"page":"503-518","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":24,"title":["Another plea for \u2018best practice\u2019 in molecular approaches to trematode systematics: <i>Diplostomum<\/i> sp. clade Q identified as <i>Diplostomum baeri<\/i> Dubois, 1937 in Europe"],"prefix":"10.1017","volume":"149","author":[{"given":"Anna","family":"Falt\u00fdnkov\u00e1","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5346-1658","authenticated-orcid":false,"given":"Olena","family":"Kudlai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila","family":"Pantoja","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Yakovleva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Lebedeva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,1,7]]},"reference":[{"key":"S0031182021002092_ref16","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"S0031182021002092_ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.019"},{"key":"S0031182021002092_ref25","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-017-2518-5"},{"key":"S0031182021002092_ref10","first-page":"S563","article-title":"A new strigeid cercaria which produces a bloat disease in tadpoles","volume":"23","author":"Cort","year":"1937","journal-title":"Journal of Parasitology"},{"key":"S0031182021002092_ref12","volume-title":"Parasitological Study of Birds","author":"Dubinina","year":"1971"},{"key":"S0031182021002092_ref50","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-015-0904-4"},{"key":"S0031182021002092_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(97)00086-6"},{"key":"S0031182021002092_ref3","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-015-9577-9"},{"key":"S0031182021002092_ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.04.018"},{"key":"S0031182021002092_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012001989"},{"key":"S0031182021002092_ref19","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"S0031182021002092_ref17","doi-asserted-by":"publisher","DOI":"10.1139\/z02-198"},{"key":"S0031182021002092_ref30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-294X.2010.04713.x"},{"key":"S0031182021002092_ref49","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182021001372"},{"key":"S0031182021002092_ref59","doi-asserted-by":"publisher","DOI":"10.1023\/A:1025546001611"},{"key":"S0031182021002092_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2019.102033"},{"key":"S0031182021002092_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(05)60003-6"},{"key":"S0031182021002092_ref14","first-page":"1","article-title":"Monographie des Strigeida (Trematoda)","volume":"6","author":"Dubois","year":"1938","journal-title":"M\u00e9moires de la Soci\u00e9t\u00e9 des Sciences Naturelles de Cherbourg"},{"key":"S0031182021002092_ref8","doi-asserted-by":"publisher","DOI":"10.1007\/s11230-016-9631-2"},{"key":"S0031182021002092_ref53","first-page":"5","article-title":"Morphology, biology and taxonomy of Diplostomum from Palearctic Laridae","volume":"27","author":"Shigin","year":"1977","journal-title":"Trudy GELAN SSSR"},{"key":"S0031182021002092_ref54","volume-title":"Trematodes in the fauna of the USSSR: the genus Diplostomum. 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