{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T04:24:57Z","timestamp":1771561497219,"version":"3.50.1"},"reference-count":56,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T00:00:00Z","timestamp":1639353600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"name":"PICT 2015, Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Argentina.","award":["0717"],"award-info":[{"award-number":["0717"]}]},{"name":"EXA 20, Universidad Nacional de Mar del Plata, Argentina","award":["975"],"award-info":[{"award-number":["975"]}]},{"name":"PICT 2019, Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica, Argentina.","award":["1123"],"award-info":[{"award-number":["1123"]}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cystic echinococcosis is a zoonotic disease caused by the larval stage of the parasite<jats:italic>Echinococcus granulosus sensu lato<\/jats:italic>. The available anti-parasitic treatment is mostly limited to a continuous administration of albendazole. However, due to its numerous side-effects and efficacy of around 50%, there is a need to find new drugs to improve the treatment for this disease. In the current study, the<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>efficacy of a<jats:italic>Stevia multiaristata<\/jats:italic>extract against<jats:italic>E. granulosus sensu stricto<\/jats:italic>(<jats:italic>s.s.<\/jats:italic>) was demonstrated.<jats:italic>Stevia multiaristata<\/jats:italic>extract (100 and 50<jats:italic>\u03bc<\/jats:italic>g mL<jats:sup>\u22121<\/jats:sup>) caused a quick viability decrease on protoscoleces which was consistent with the observed tegumental alterations. Loss of turgidity was detected in 95 \u00b1 3.4% of cysts incubated with<jats:italic>S. multiaristata<\/jats:italic>extract during 2 days (100<jats:italic>\u03bc<\/jats:italic>g mL<jats:sup>\u22121<\/jats:sup>) and the collapse of the germinal layer was observed in 60 \u00b1 9.3% of cysts treated with 100<jats:italic>\u03bc<\/jats:italic>g mL<jats:sup>\u22121<\/jats:sup>of the<jats:italic>S. multiaristata<\/jats:italic>extract during 4 days. The half maximal effective concentration value was 69.6<jats:italic>\u03bc<\/jats:italic>g mL<jats:sup>\u22121<\/jats:sup>and the selectivity index for<jats:italic>E. granulosus s.s.<\/jats:italic>cysts was 1.9. In this clinical efficacy study, the treatment of infected mice with the<jats:italic>S. multiaristata<\/jats:italic>extract (50 mg kg<jats:sup>\u22121<\/jats:sup>) caused a significant decrease in the weight of the cysts compared with the control group. These results coincided with the tissue damage observed in the cysts at the ultrastructural level. In conclusion, we observed high protoscolicidal and cysticidal effects, and significant reduction in the weight of the cysts in experimentally infected mice following treatment with the<jats:italic>S. multiaristata<\/jats:italic>extract.<\/jats:p>","DOI":"10.1017\/s0031182021002109","type":"journal-article","created":{"date-parts":[[2021,12,13]],"date-time":"2021-12-13T01:09:35Z","timestamp":1639357775000},"page":"519-528","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Anthelmintic activity of<i>Stevia multiaristata<\/i>extract against<i>Echinococcus granulosus sensu stricto<\/i>"],"prefix":"10.1017","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4424-7019","authenticated-orcid":false,"given":"C. M.","family":"Albani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Borgo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Fabbri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Pensel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Fasciani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Elso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Papademetrio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Grasso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Paladini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. F.","family":"Beer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5190-370X","authenticated-orcid":false,"given":"N. E.","family":"Farias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Elissondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Gambino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Zoppi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"S\u00fclsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Elissondo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2021,12,13]]},"reference":[{"key":"S0031182021002109_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.11.001"},{"key":"S0031182021002109_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(76)90437-X"},{"key":"S0031182021002109_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2008.03.037"},{"key":"S0031182021002109_ref8","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26092733"},{"key":"S0031182021002109_ref36","volume-title":"Guide for the Care and Use of Laboratory Animals","author":"National Research Council US","year":"2011"},{"key":"S0031182021002109_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2019.101335"},{"key":"S0031182021002109_ref47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006422"},{"key":"S0031182021002109_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2010.11.010"},{"key":"S0031182021002109_ref48","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26051251"},{"key":"S0031182021002109_ref45","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.0c01050"},{"key":"S0031182021002109_ref25","doi-asserted-by":"publisher","DOI":"10.1080\/08941939.2018.1459967"},{"key":"S0031182021002109_ref33","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02963-14"},{"key":"S0031182021002109_ref51","doi-asserted-by":"publisher","DOI":"10.2174\/1871526518666180509130838"},{"key":"S0031182021002109_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2018.10.001"},{"key":"S0031182021002109_ref21","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.09.001"},{"key":"S0031182021002109_ref4","doi-asserted-by":"publisher","DOI":"10.3109\/13880209.2016.1150304"},{"key":"S0031182021002109_ref27","doi-asserted-by":"publisher","DOI":"10.1016\/j.apjtm.2016.01.038"},{"key":"S0031182021002109_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2020.108051"},{"key":"S0031182021002109_ref7","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6383"},{"key":"S0031182021002109_ref44","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-78274-4_8"},{"key":"S0031182021002109_ref54","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00075-18"},{"key":"S0031182021002109_ref17","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0381-y"},{"key":"S0031182021002109_ref23","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033368583"},{"key":"S0031182021002109_ref35","doi-asserted-by":"publisher","DOI":"10.3390\/app10093001"},{"key":"S0031182021002109_ref41","doi-asserted-by":"crossref","first-page":"248532","DOI":"10.1155\/2013\/248532","article-title":"Targeting apoptosis pathways in cancer with alantolactone and isoalantolactone","volume":"27","author":"Rasul","year":"2013","journal-title":"Scientific World Journal"},{"key":"S0031182021002109_ref39","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/693289"},{"key":"S0031182021002109_ref46","first-page":"633","article-title":"IFCC methods for the measurement of catalytic concentration of enzymes. Part 4. IFCC method for gamma-glutamyltransferase [(gamma-glutamyl)-peptide: amino acid gamma-glutamyltransferase, EC 2.3.2.2]","volume":"21","author":"Shaw","year":"1983","journal-title":"Journal of Clinical Chemistry and Clinical Biochemistry"},{"key":"S0031182021002109_ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s11523-013-0256-y"},{"key":"S0031182021002109_ref56","volume-title":"Cat\u00e1logo de las Plantas Vasculares del Cono Sur (Argentina, Sur de Brasil, Chile, Paraguay y Uruguay)","volume":"2","author":"Zuloaga","year":"2008"},{"key":"S0031182021002109_ref32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00263.x"},{"key":"S0031182021002109_ref49","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24071209"},{"key":"S0031182021002109_ref38","first-page":"20","volume-title":"WHO\/OIE Manual on Echinococcosis in Humans and Animals: A Public Health Problem of Global Concern","author":"Pawlowski","year":"2001"},{"key":"S0031182021002109_ref43","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2015.1072083"},{"key":"S0031182021002109_ref30","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0596-9"},{"key":"S0031182021002109_ref20","doi-asserted-by":"publisher","DOI":"10.3390\/molecules25092014"},{"key":"S0031182021002109_ref31","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3125-1"},{"key":"S0031182021002109_ref55","unstructured":"WHO (2013) WHO Traditional Medicine Strategy 2014\u20132023. Available at http:\/\/www.who.int\/medicines\/publications\/traditional\/trm_strategy14_23\/en\/ (Accessed 25 September 2021)."},{"key":"S0031182021002109_ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-009-1469-y"},{"key":"S0031182021002109_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43072-4"},{"key":"S0031182021002109_ref10","doi-asserted-by":"crossref","first-page":"456","DOI":"10.3746\/jkfn.2012.41.4.456","article-title":"Anti-inflammatory activity of austroinulin from Stevia rebaudiana in LPS-induced RAW264.7 cells","volume":"41","author":"Byoung","year":"2012","journal-title":"Journal of the Korean Society of Food Science and Nutrition"},{"key":"S0031182021002109_ref3","doi-asserted-by":"crossref","unstructured":"Alves, KS (2020) ec50estimator: an automated way to estimate EC50 for stratified datasets. R package version 0.1.0. Available at https:\/\/CRAN.R-project.org\/package=ec50estimator (Accessed 13 August 2021).","DOI":"10.32614\/CRAN.package.ec50estimator"},{"key":"S0031182021002109_ref29","unstructured":"Lenth, RV (2021). Emmeans: Estimated Marginal Means, aka Least-Squares Means. R package version 1.6.1. Available at https:\/\/CRAN.R-project.org\/package=emmeans (Accessed 13 August 2021)."},{"key":"S0031182021002109_ref52","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(01)50032-9"},{"key":"S0031182021002109_ref40","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-S1-S4"},{"key":"S0031182021002109_ref37","first-page":"41","article-title":"Cystic echinococcosis in South America: a call for action","volume":"21","author":"Pavletic","year":"2017","journal-title":"Revista Panamericana de Salud P\u00fablica"},{"key":"S0031182021002109_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.04.003"},{"key":"S0031182021002109_ref22","volume-title":"An {R} Companion to Applied Regression","author":"Fox","year":"2019"},{"key":"S0031182021002109_ref15","first-page":"183","volume-title":"Natural Products from Plants","author":"Duke","year":"1999"},{"key":"S0031182021002109_ref16","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-005-0026-6"},{"key":"S0031182021002109_ref26","doi-asserted-by":"crossref","first-page":"258","DOI":"10.4103\/0974-8490.69128","article-title":"Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats","volume":"2","author":"Kujur","year":"2010","journal-title":"Pharmacognosy Research"},{"key":"S0031182021002109_ref42","volume-title":"R: A Language and Environment for Statistical Computing","year":"2021"},{"key":"S0031182021002109_ref2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0240456"},{"key":"S0031182021002109_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.105050"},{"key":"S0031182021002109_ref53","first-page":"351","volume-title":"Emerging Trends in Zoology","author":"Tandon","year":"2011"},{"key":"S0031182021002109_ref50","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/15.2.124"},{"key":"S0031182021002109_ref19","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2012.12.007"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182021002109","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,14]],"date-time":"2024-09-14T08:07:43Z","timestamp":1726301263000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182021002109\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12,13]]},"references-count":56,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["S0031182021002109"],"URL":"https:\/\/doi.org\/10.1017\/s0031182021002109","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12,13]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2021. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}