{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:48:38Z","timestamp":1789778918966,"version":"4.0.1"},"reference-count":22,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA164719"],"award-info":[{"award-number":["R01CA164719"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004071","name":"Khon Kaen University","doi-asserted-by":"publisher","award":["RP64018"],"award-info":[{"award-number":["RP64018"]}],"id":[{"id":"10.13039\/501100004071","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012309","name":"Royal Golden Jubilee (RGJ) Ph.D. Programme","doi-asserted-by":"publisher","award":["PHD\/0013\/2555"],"award-info":[{"award-number":["PHD\/0013\/2555"]}],"id":[{"id":"10.13039\/501100012309","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent reports implicate both the liver fluke <jats:italic>Opisthorchis viverrini<\/jats:italic> as a reservoir of <jats:italic>Helicobacter pylori<\/jats:italic> within the human gastrointestinal tract and <jats:italic>H. pylori<\/jats:italic> in the pathogenesis of opisthorchiasis-associated cholangiocarcinoma. We postulated that adherence of bacterial ligands to host receptors initiates colonization of the live fluke by <jats:italic>H. pylori<\/jats:italic> and here we aimed to assess the molecular interaction between <jats:italic>O<\/jats:italic>. <jats:italic>viverrini<\/jats:italic> and <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> by investigating host receptors for <jats:italic>H. pylori<\/jats:italic> in the fluke. Several known receptors of <jats:italic>H. pylori<\/jats:italic> including Lewis B, sialyl-Lewis X, Toll-like receptor 4 and L-fucose were detected immunohistochemically and histochemically by focusing analysis on the gut epithelium and tegument of the adult stage of the fluke. The frequency of detection of Lewis B, sialyl-Lewis X, TLR4 and L-fucose in 100 individual worms was 3, 3, 19 and 70%, respectively. Detection of <jats:italic>H. pylori<\/jats:italic> by a diagnostic <jats:italic>ureA<\/jats:italic> gene-based PCR assay revealed the presence of <jats:italic>H. pylori<\/jats:italic> in individual <jats:italic>O. viverrini<\/jats:italic> worms in 41 of 49 (79%) worms examined. In addition, numbers of bacteria decreased in a dose- and time-dependent fashion following exposure to fucosidase. These findings suggested that L-fucose represents a tractable receptor for <jats:italic>H. pylori<\/jats:italic> that can mediate bacterial colonization of the gut of <jats:italic>O. viverrini<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182022000592","type":"journal-article","created":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T11:54:56Z","timestamp":1651233296000},"page":"1374-1379","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":6,"title":["Adherence of <i>Helicobacter pylori<\/i> to <i>Opisthorchis viverrini<\/i> gut epithelium and the tegument mediated <i>via<\/i> L-fucose binding adhesin"],"prefix":"10.1017","volume":"149","author":[{"given":"Prissadee","family":"Thanaphongdecha","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaovalux","family":"Chamgramol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chawalit","family":"Pairojkul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raksawan","family":"Deenonpoe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2323-2344","authenticated-orcid":false,"given":"Sutas","family":"Suttiprapa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Brindley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8899-5919","authenticated-orcid":false,"given":"Banchob","family":"Sripa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,4,29]]},"reference":[{"key":"S0031182022000592_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bj.2015.06.002"},{"key":"S0031182022000592_ref17","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.656439"},{"key":"S0031182022000592_ref6","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00009-17"},{"key":"S0031182022000592_ref21","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10091089"},{"key":"S0031182022000592_ref18","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.27001"},{"key":"S0031182022000592_ref10","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00050-17"},{"key":"S0031182022000592_ref16","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069076"},{"key":"S0031182022000592_ref4","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0077358"},{"key":"S0031182022000592_ref20","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2016.11.016"},{"key":"S0031182022000592_ref9","doi-asserted-by":"publisher","DOI":"10.1038\/srep42744"},{"key":"S0031182022000592_ref11","doi-asserted-by":"publisher","DOI":"10.1080\/23744235.2019.1588472"},{"key":"S0031182022000592_ref12","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.5.2035"},{"key":"S0031182022000592_ref5","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12638"},{"key":"S0031182022000592_ref19","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-232751"},{"key":"S0031182022000592_ref8","doi-asserted-by":"publisher","DOI":"10.7314\/APJCP.2015.16.5.1751"},{"key":"S0031182022000592_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.013"},{"key":"S0031182022000592_ref2","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12335"},{"key":"S0031182022000592_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.02.008"},{"key":"S0031182022000592_ref1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1098801"},{"key":"S0031182022000592_ref14","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5349.373"},{"key":"S0031182022000592_ref7","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12817"},{"key":"S0031182022000592_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1477-2574.2011.00423.x"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182022000592","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T09:06:42Z","timestamp":1677834402000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182022000592\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,29]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["S0031182022000592"],"URL":"https:\/\/doi.org\/10.1017\/s0031182022000592","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,29]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2022. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}}]}}