{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T13:11:30Z","timestamp":1774530690505,"version":"3.50.1"},"reference-count":19,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T00:00:00Z","timestamp":1652659200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yunnan Province Medical Discipline Reserve Talent Training Program","award":["H-2018073"],"award-info":[{"award-number":["H-2018073"]}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The parasite <jats:italic>Fasciola hepatica<\/jats:italic> is an important zoonotic parasite. The development of an animal model of <jats:italic>F. hepatica<\/jats:italic>'s life cycle is critical for studying the biological characteristics of the parasite in snails and mammals. Eggs of <jats:italic>F. hepatica<\/jats:italic> of bovine origin were cultured, and metacercariae were obtained after infection of <jats:italic>Galba pervia<\/jats:italic> snails. The life cycle system of <jats:italic>F. hepatica<\/jats:italic> was initiated in 2 different animals by orally infecting rabbits, SD rats and Kunming mice with the metacercariae. The animals' survival after infection, parasite migration in the animals and pathological damage to the liver were observed. We discovered that rabbits died due to acute suppurative hepatitis 60<jats:italic>\u2013<\/jats:italic>69 days after infection, and eggs were found in the feces on day 63 of infection. The liver of SD rats showed punctate lesions on day 3 of infection, and further changes occurred as the infection progressed. However, liver repair was observed at week 9. SD rats survived for more than a year after infection and continued the <jats:italic>F. hepatica<\/jats:italic> life cycle. The liver lesions in Kunming mice after infection were similar but more severe than those in SD rats. Death was observed on the 31st post<jats:italic>-<\/jats:italic>infection day. We discovered that while rabbits, SD rats and Kunming mice can all be used as animal models of <jats:italic>F. hepatica<\/jats:italic>, SD rats are more suitable experimental animals in terms of tolerance and pathological response.<\/jats:p>","DOI":"10.1017\/s0031182022000609","type":"journal-article","created":{"date-parts":[[2022,5,16]],"date-time":"2022-05-16T07:15:20Z","timestamp":1652685320000},"page":"1349-1355","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Exploration of animal models to study the life cycle of <i>Fasciola hepatica<\/i>"],"prefix":"10.1017","volume":"149","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7573-308X","authenticated-orcid":false,"given":"Wen","family":"Fang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-yin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shao-rong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ke-rong","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Duan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3718-0378","authenticated-orcid":false,"given":"Tian-mei","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2022,5,16]]},"reference":[{"key":"S0031182022000609_ref19","first-page":"24","article-title":"Comparison and differentiation between Fascioles","volume":"1","author":"Yin","year":"1995","journal-title":"Haixia Journal of Preventive Medicine"},{"key":"S0031182022000609_ref7","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.579801"},{"key":"S0031182022000609_ref14","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.793571"},{"key":"S0031182022000609_ref17","first-page":"525","article-title":"Molecular identification of Fasciola flukes in Dali prefecture, Yunnnan Province","volume":"14","author":"Tang","year":"2019","journal-title":"Journal of Pathogen Biology"},{"key":"S0031182022000609_ref6","first-page":"285","article-title":"Experimental infection of Galba pervia, Radix swinhoei, and Physa acuta with Fasciola hepatica in Dali, Yunnan","volume":"32","author":"Fang","year":"2014","journal-title":"Chinese Journal of Parasitology Diseases"},{"key":"S0031182022000609_ref15","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.21-0539"},{"key":"S0031182022000609_ref5","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00022343"},{"key":"S0031182022000609_ref18","first-page":"389","volume-title":"Human Parasitology","author":"Wu","year":"2005"},{"key":"S0031182022000609_ref13","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00023385"},{"key":"S0031182022000609_ref16","doi-asserted-by":"publisher","DOI":"10.1515\/ap-2018-0053"},{"key":"S0031182022000609_ref1","first-page":"791","article-title":"Surveillance and molecular identification of Fasciola spp. from cattle and goats at Binchuan, Yunnan province","volume":"13","author":"Ai","year":"2013","journal-title":"The Journal of Tropical 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Medicine"},{"key":"S0031182022000609_ref4","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA117.000445"},{"key":"S0031182022000609_ref3","first-page":"116","volume-title":"Human Parasitology Experiment 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\u00a9 The Author(s), 2022. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}