{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T07:40:25Z","timestamp":1782200425346,"version":"3.54.5"},"reference-count":84,"publisher":"Cambridge University Press (CUP)","issue":"11","license":[{"start":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T00:00:00Z","timestamp":1656979200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The phylum Platyhelminthes shares a unique population of undifferentiated cells responsible for the proliferation capacity needed for cell renewal, growth, tissue repair and regeneration. These cells have been extensively studied in free-living flatworms, whereas in cestodes the presence of a set of undifferentiated cells, known as germinative cells, has been demonstrated in classical morphology studies, but poorly characterized with molecular biology approaches. Furthermore, several genes have been identified as neoblast markers in free-living flatworms that deserve study in cestode models. Here, different cell types of the model cestode <jats:italic>Mesocestoides corti<\/jats:italic> were characterized, identifying differentiated and germinative cells. Muscle cells, tegumental cells, calcareous corpuscle precursor cells and excretory system cells were identified, all of which are non-proliferative, differentiated cell types. Besides those, germinative cells were identified as a population of small cells with proliferative capacity <jats:italic>in vivo<\/jats:italic>. Primary cell culture experiments in Dulbecco's Modified Eagle Medium (DMEM), <jats:italic>Echinococcus<\/jats:italic> hydatid fluid and hepatocyte conditioned media in non-reductive or reductive conditions confirmed that the germinative cells were the only ones with proliferative capacity. Since several genes have been identified as markers of undifferentiated neoblast cells in free-living flatworms, the expression of <jats:italic>pumilio<\/jats:italic> and <jats:italic>pL10<\/jats:italic> genes was analysed by qPCR and <jats:italic>in situ<\/jats:italic> hybridization, showing that the expression of these genes was stronger in germinative cells but not restricted to this cell type. This study provides the first tools to analyse and further characterise undifferentiated cells in a model cestode.<\/jats:p>","DOI":"10.1017\/s0031182022000956","type":"journal-article","created":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T07:52:52Z","timestamp":1657007572000},"page":"1505-1514","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Cell repertoire and proliferation of germinative cells of the model cestode <i>Mesocestoides corti<\/i>"],"prefix":"10.1017","volume":"149","author":[{"given":"Mar\u00eda Fernanda","family":"Dom\u00ednguez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Cost\u00e1bile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uriel","family":"Koziol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mat\u00edas","family":"Preza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Brehm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 F.","family":"Tort","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6053-7585","authenticated-orcid":false,"given":"Estela","family":"Castillo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2022,7,5]]},"reference":[{"key":"S0031182022000956_ref39","first-page":"121","volume-title":"Research in Helminths","author":"Koziol","year":"2011"},{"key":"S0031182022000956_ref31","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0022-5320(75)90003-9","article-title":"Fine structure studies on the planarian, Dugesia: I. Nature of the \u2018neoblast\u2019 and other cell types in non injured worms","volume":"50","author":"Hay","year":"1975","journal-title":"Journal of Ultrastructure Research"},{"key":"S0031182022000956_ref54","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1046\/j.1440-169X.2002.00634.x","article-title":"Planarian fibroblast growth factor receptor homologs expressed in stem cells and cephalic ganglions","volume":"44","author":"Ogawa","year":"2002","journal-title":"Development, Growth and Differentiation"},{"key":"S0031182022000956_ref75","first-page":"449","article-title":"The development of organ systems in cestodes I: a study of histology of Hymenolepis diminuta (Rudolphi, 1819)","volume":"20","author":"Sulgostowska","year":"1972","journal-title":"Acta Parasitologica Polonica"},{"key":"S0031182022000956_ref61","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1126\/science.1116110","article-title":"SMEDWI-2 is a PIWI-like protein that regulates planarian stem cells","volume":"310","author":"Reddien","year":"2005","journal-title":"Science"},{"key":"S0031182022000956_ref3","doi-asserted-by":"crossref","first-page":"267","DOI":"10.2307\/3278024","article-title":"Developmental physiology of cestodes. 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Cytological characteristics of the germinative region of Hymenolepis diminuta","volume":"57","author":"Bolla","year":"1971","journal-title":"The Journal of Parasitology"},{"key":"S0031182022000956_ref34","doi-asserted-by":"crossref","first-page":"661","DOI":"10.5962\/bhl.part.82399","article-title":"Ultrastructural study of the tetrathyridium of Mesocestoides corti Hoeppli, 1925 (Cestoda): pool of germinative cells and suckers","volume":"88","author":"Hess","year":"1981","journal-title":"Revue Suisse de Zoologie"},{"key":"S0031182022000956_ref44","doi-asserted-by":"crossref","first-page":"2136","DOI":"10.1093\/gbe\/evv126","article-title":"A novel terminal-repeat retrotransposon in miniature (TRIM) is massively expressed in Echinococcus multilocularis stem cells","volume":"7","author":"Koziol","year":"2015","journal-title":"Genome Biology and 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Acta AcademiaeAboensis 50, 13\u201344."},{"key":"S0031182022000956_ref1","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.tcb.2011.01.005","article-title":"Planarian stem cells: a simple paradigm for regeneration","volume":"21","author":"Aboobaker","year":"2011","journal-title":"Trends in Cell Biology"},{"key":"S0031182022000956_ref48","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1038\/335611a0","article-title":"The product of the Drosophila gene vasa is very similar to eukaryotic initiation factor-4A","volume":"335","author":"Lasko","year":"1988","journal-title":"Nature"},{"key":"S0031182022000956_ref12","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1093\/emboj\/16.4.834","article-title":"A CCHC metal-binding domain in Nanos is essential for translational regulation","volume":"16","author":"Curtis","year":"1997","journal-title":"The EMBO Journal"},{"key":"S0031182022000956_ref41","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/1742-9994-7-22","article-title":"Stem cell proliferation during in vitro development of the model cestode Mesocestoides corti from larva to adult worm","volume":"7","author":"Koziol","year":"2010","journal-title":"Frontiers in Zoology"},{"key":"S0031182022000956_ref5","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1017\/S0022149X00000433","article-title":"Effect of praziquantel on the strobilar development of Mesocestoides corti in vitro","volume":"74","author":"Britos","year":"2000","journal-title":"Journal of Helminthology"},{"key":"S0031182022000956_ref56","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0925-4773(97)00099-3","article-title":"A vasa-like gene in zebrafish identifies putative primordial germ cells","volume":"66","author":"Olsen","year":"1997","journal-title":"Mechanisms of Development"},{"key":"S0031182022000956_ref66","doi-asserted-by":"crossref","first-page":"e48958","DOI":"10.7554\/eLife.48958","article-title":"Region-specific regulation of stem cell-driven regeneration in tapeworms","volume":"8","author":"Rozario","year":"2019","journal-title":"eLife"},{"key":"S0031182022000956_ref73","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1007\/s00436-003-1046-8","article-title":"Long-term in vitro cultivation of Echinococcus multilocularis metacestodes under axenic conditions","volume":"92","author":"Spiliotis","year":"2004","journal-title":"Parasitology Research"},{"key":"S0031182022000956_ref19","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1186\/s12862-017-1061-5","article-title":"Conservation and diversification of small RNA pathways within flatworms","volume":"17","author":"Fontenla","year":"2017","journal-title":"BMC Evolutionary Biology"},{"key":"S0031182022000956_ref28","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1007\/BF02462976","article-title":"Studies on cytodifferentiation in the neck region of Diphyllobothrium dendriticum Nitzsch, 1824 (Cestoda, Pseudophyllidea)","volume":"50","author":"Gustafsson","year":"1976","journal-title":"Zeitschrift f\u00fcr Parasitenkunde"},{"key":"S0031182022000956_ref23","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0378-1119(94)90541-X","article-title":"Mouse erythroid cells express multiple putative RNA helicase genes exhibiting high sequence conservation from yeast to mammals","volume":"140","author":"Gee","year":"1994","journal-title":"Gene"},{"key":"S0031182022000956_ref13","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1007\/BF00577724","article-title":"A quantitative method for maceration of hydra tissue","volume":"171","author":"David","year":"1973","journal-title":"Wilhelm Roux\u2019 Archiv f\u00fcr Entwicklungsmechanik der Organismen"},{"key":"S0031182022000956_ref27","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/349717a0","article-title":"The Xenopus localized messenger RNA An3 may encode an ATP-dependent RNA helicase","volume":"349","author":"Gururajan","year":"1991","journal-title":"Nature"},{"key":"S0031182022000956_ref7","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1016\/j.ijpara.2017.03.005","article-title":"Identification of EmSOX2, a member of the Sox family of transcription factors, as a potential regulator of Echinococcus multilocularis germinative cells","volume":"47","author":"Cheng","year":"2017","journal-title":"International Journal for Parasitology"},{"key":"S0031182022000956_ref53","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/S0022-5320(69)90017-3","article-title":"Electron microscopic studies of planarian regeneration. 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