{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:00:40Z","timestamp":1740135640200,"version":"3.37.3"},"reference-count":29,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["303008\/2018-0"],"award-info":[{"award-number":["303008\/2018-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Verminotic pneumonia caused by <jats:italic>Parafilaroides<\/jats:italic> spp. nematodes is an underreported disease in beached South American fur seals, with scant literature available on the characteristics of parafilaroidiasis, the nematode itself, as well as its occurrence in pinnipeds in Brazil. The present work aims to identify, describe and detail the histological features of the infection and molecular characteristics of verminotic pneumonia in the South American fur seal. Twenty-six specimens of <jats:italic>Arctocephalus australis<\/jats:italic>, found dead on the northern coast of Rio Grande do Sul in 2021, were analysed. These animals were identified and submitted to necropsy and histology. For the molecular identification of metastrongylids, lung fragments were subjected to DNA extraction, polymerase chain reaction targeting the <jats:italic>Internal transcribed spacer 2 (ITS-2)<\/jats:italic> gene and subsequent sequencing. In total, 12 animals presented with parasites in the lung parenchyma on histological evaluation, and only 1 showed a granulomatous lung lesion at necropsy. Microscopically, the nematodes were found mainly in the alveoli, associated with little or no inflammatory response, and they had morphological characteristics compatible with metastrongylids. Six <jats:italic>ITS-2<\/jats:italic> gene quality sequences were obtained; after comparative analysis <jats:italic>via<\/jats:italic> BLAST, they showed similarity with sequences obtained from <jats:italic>Parafilaroides<\/jats:italic> sp. Therefore, verminotic pneumonia caused by <jats:italic>Parafilaroides<\/jats:italic> represents an important differential diagnosis of lung disease in South American fur seals found on the northern coast of Rio Grande do Sul.<\/jats:p>","DOI":"10.1017\/s0031182022001494","type":"journal-article","created":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T23:52:06Z","timestamp":1667346726000},"page":"150-156","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Verminotic pneumonia in South American fur seal (<i>Arctocephalus australis<\/i>) in Southern Brazil"],"prefix":"10.1017","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9365-2002","authenticated-orcid":false,"given":"Yasmin","family":"Daoualibi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2244-9848","authenticated-orcid":false,"given":"Renata F.","family":"Moreira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcele B.","family":"Bandinelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna V. Z.","family":"Echenique","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo G. C.","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o F.","family":"Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saulo P.","family":"Pavarini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2022,11,1]]},"reference":[{"key":"S0031182022001494_ref7","doi-asserted-by":"publisher","DOI":"10.1201\/9781420041637.ch18"},{"key":"S0031182022001494_ref22","doi-asserted-by":"publisher","DOI":"10.3354\/dao03291"},{"key":"S0031182022001494_ref21","doi-asserted-by":"publisher","DOI":"10.1590\/s1984-29612019029"},{"key":"S0031182022001494_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2011.01.006"},{"key":"S0031182022001494_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2007.04.018"},{"key":"S0031182022001494_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2019.10.009"},{"key":"S0031182022001494_ref8","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3525.1"},{"key":"S0031182022001494_ref16","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"S0031182022001494_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.12.008"},{"key":"S0031182022001494_ref25","doi-asserted-by":"publisher","DOI":"10.7589\/2017-12-291"},{"key":"S0031182022001494_ref24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-7692.2011.00534.x"},{"key":"S0031182022001494_ref15","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-41.3.593"},{"key":"S0031182022001494_ref14","first-page":"1","article-title":"Pathological and parasitological findings in south American fur seal pups (Arctocephalus australis) in Uruguay","volume":"2012","author":"Katz","year":"2012","journal-title":"International Scholarly Research Network"},{"key":"S0031182022001494_ref27","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000085024"},{"key":"S0031182022001494_ref3","doi-asserted-by":"publisher","DOI":"10.1201\/9781315151588-17"},{"key":"S0031182022001494_ref10","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X20000826"},{"key":"S0031182022001494_ref18","doi-asserted-by":"publisher","DOI":"10.1002\/9780470377000.ch10"},{"volume-title":"Georgis\u2019 Parasitology for Veterinarians","year":"2014","author":"Bowman","key":"S0031182022001494_ref5"},{"key":"S0031182022001494_ref11","doi-asserted-by":"publisher","DOI":"10.1177\/104063879700900311"},{"key":"S0031182022001494_ref20","unstructured":"Ponce de Le\u00f3n, A (1983) Crecimiento Intrauterino y Postnataldel Lobo de dos Pelos Sudamericano, Arctocephalus australis (Zimmerman, 1783), en Islas de Uruguay. In Actas del VIII Simposio Latinoamericano de Oceaograf\u00eda Biol\u00f3gica. Montevideo, Uruguay."},{"volume-title":"Marine Mammals Ashore: A Field Guide for Strandings","year":"2005","author":"Geraci","key":"S0031182022001494_ref12"},{"key":"S0031182022001494_ref6","first-page":"215","article-title":"The transmission of Parafilaroides decorus (Nematoda: Metastrongyloidea) in the California sea lion (Zalophus californianus)","volume":"37","author":"Dailey","year":"1970","journal-title":"Proceedings of the Helminthological Society of Washington"},{"key":"S0031182022001494_ref13","doi-asserted-by":"publisher","DOI":"10.1638\/2015-0090.1"},{"key":"S0031182022001494_ref19","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-25.2.218"},{"key":"S0031182022001494_ref1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"S0031182022001494_ref28","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"S0031182022001494_ref2","doi-asserted-by":"publisher","DOI":"10.4067\/S0301-732X2016000100006"},{"key":"S0031182022001494_ref9","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1521.1"},{"key":"S0031182022001494_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2019.04.001"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182022001494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,3]],"date-time":"2023-02-03T03:22:37Z","timestamp":1675394557000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182022001494\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,1]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["S0031182022001494"],"URL":"https:\/\/doi.org\/10.1017\/s0031182022001494","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2022,11,1]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2022. 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