{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:00:43Z","timestamp":1740135643490,"version":"3.37.3"},"reference-count":20,"publisher":"Cambridge University Press (CUP)","issue":"8","license":[{"start":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T00:00:00Z","timestamp":1676592000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004071","name":"Khon Kaen University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004071","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Opisthorchis viverrini<\/jats:italic> is a carcinogenic parasite that can cause bile duct cancer called cholangiocarcinoma. A study of the immune response of this parasite in susceptible and non-susceptible hosts may provide a clue to develop vaccines and immunodiagnostic markers, which are currently not available. Here, we compared the antibody response in susceptible Golden Syrian hamsters and non-susceptible BALB\/c mice infected by the liver fluke. In mice, the antibody was detected between 1 and 2 weeks post-infection, whereas it was positive between 2 and 4 weeks post-infection in hamsters. Immunolocalization revealed that the antibody from mice reacts strongly with the tegumental surface and gut epithelium of the worm, while hamster antibody showed a weak signal in the tegument and a comparable signal in the gut of the worm. Immunoblot of the tegumental proteins demonstrated that while hamster antibody showed a broad specificity, mice strongly reacted with a single protein band. Mass spectrometry revealed these immunogenic targets. Recombinant proteins of the reactive targets were produced in the bacterial expression system. The immunoblot of these recombinant proteins confirm the reactivity of their native form. In summary, there is a different antibody response against <jats:italic>O. viverrini<\/jats:italic> infection in susceptible and non-susceptible hosts. The non-susceptible host reacts quicker and stronger than the susceptible host.<\/jats:p>","DOI":"10.1017\/s0031182023000112","type":"journal-article","created":{"date-parts":[[2023,2,17]],"date-time":"2023-02-17T07:47:30Z","timestamp":1676620050000},"page":"653-660","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Distinct antibody response in susceptible and non-susceptible hosts of the carcinogenic liver fluke <i>Opisthorchis viverrini<\/i> infection"],"prefix":"10.1017","volume":"150","author":[{"given":"Krongkarn","family":"Watakulsin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpa","family":"Surapaitoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorina Handayani","family":"Ulag","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunheng","family":"Kaing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Watcharapol","family":"Suyapoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prasert","family":"Saichua","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanin","family":"Salao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sirikachorn","family":"Tangkawatana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2323-2344","authenticated-orcid":false,"given":"Sutas","family":"Suttiprapa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2023,2,17]]},"reference":[{"key":"S0031182023000112_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(06)63003-0"},{"key":"S0031182023000112_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00054-0"},{"key":"S0031182023000112_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2021.106133"},{"key":"S0031182023000112_ref17","first-page":"2437","article-title":"Granulin expression in hamsters during Opisthorchis viverrini infection-induced cholangiocarcinogenesis","volume":"19","author":"Upontain","year":"2018","journal-title":"Asian Pacific Journal of Cancer Prevention: APJCP"},{"key":"S0031182023000112_ref9","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines9070740"},{"key":"S0031182023000112_ref20","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5378"},{"key":"S0031182023000112_ref10","first-page":"243","article-title":"Humoral immune responses in hamsters infected with Opisthorchis viverrini","volume":"14","author":"Sirisinha","year":"1983","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182023000112_ref14","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2000.00286.x"},{"key":"S0031182023000112_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2011.08.005"},{"key":"S0031182023000112_ref8","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00213"},{"key":"S0031182023000112_ref3","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182099005764"},{"key":"S0031182023000112_ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2016.03.079"},{"key":"S0031182023000112_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(86)90112-8"},{"key":"S0031182023000112_ref18","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X00009913"},{"key":"S0031182023000112_ref7","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900393"},{"key":"S0031182023000112_ref19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000719"},{"key":"S0031182023000112_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2018.10.001"},{"key":"S0031182023000112_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.10.002"},{"key":"S0031182023000112_ref1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2015.61"},{"key":"S0031182023000112_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(81)90421-1"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182023000112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,24]],"date-time":"2023-07-24T11:59:02Z","timestamp":1690199942000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182023000112\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,17]]},"references-count":20,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2023,7]]}},"alternative-id":["S0031182023000112"],"URL":"https:\/\/doi.org\/10.1017\/s0031182023000112","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2023,2,17]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2023. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}