{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T06:01:45Z","timestamp":1774936905874,"version":"3.50.1"},"reference-count":28,"publisher":"Cambridge University Press (CUP)","issue":"9","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The tapeworms of <jats:italic>Moniezia<\/jats:italic> spp. are heteroxenous parasites and their adult forms occur in ruminants' alimentary tract. They steal a significant portion of hosts' nourishment initiating monieziasis, thereby inflicting economic losses in animal rearing. Despite their high economic importance, the molecular characterization and taxonomic status of these parasites have remained poorly understood. In the present study, cestodes were isolated from the sheep and goats' intestines and were stained with Gower's carmine. Upon careful evaluation of morphological characters, 2 species <jats:italic>Moniezia denticulata<\/jats:italic> and <jats:italic>Moniezia expansa<\/jats:italic> were identified. The genomic DNA was extracted and polymerase chain reaction (PCR) amplified targeting regions of mitochondrial cytochrome c oxidase subunit 1 (<jats:italic>cox1<\/jats:italic>), small subunit ribosomal RNA (SSU rRNA) and internal transcribed spacer 1\u20135.8S rRNA (ITS1\u20135.8S rRNA) genes followed by sequencing. The partial sequences of <jats:italic>cox1<\/jats:italic>, SSU rRNA and ITS1\u20135.8S rRNA genes of <jats:italic>M. denticulata<\/jats:italic> generated in the present study revealed that even though they share high similarities with <jats:italic>M. benedeni<\/jats:italic> (93.2% <jats:italic>cox1<\/jats:italic>; 92.6% SSU rRNA; 84.70% ITS1\u20135.8S rRNA) and <jats:italic>M. expansa<\/jats:italic> (88.85% <jats:italic>cox1<\/jats:italic>; 92.27% SSU rRNA; 81.70% ITS1\u20135.8S rRNA), they are not identical to them. In the maximum likelihood phylogenetic trees, <jats:italic>M. denticulata<\/jats:italic> and <jats:italic>M. expansa<\/jats:italic> consistently appeared as distinct species from each other. The high values of pairwise divergence between these 2 species collected in the present study confirmed their separate identity. The present study reports the first molecular characterization of <jats:italic>M. denticulata<\/jats:italic> with reference to <jats:italic>M. expansa<\/jats:italic> infecting sheep and goats in India.<\/jats:p>","DOI":"10.1017\/s003118202300063x","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T11:13:06Z","timestamp":1689765186000},"page":"831-841","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["Molecular characterization of <i>Moniezia denticulata<\/i> (Rudolphi, 1810) and its distinction from <i>M. expansa<\/i> infecting sheep and goats raised in the north and north-western regions of India"],"prefix":"10.1017","volume":"150","author":[{"given":"Susheel","family":"Kumar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0047-7146","authenticated-orcid":false,"given":"Harpreet","family":"Kaur","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"S003118202300063X_ref4","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0388-4"},{"key":"S003118202300063X_ref12","doi-asserted-by":"publisher","DOI":"10.1007\/BF01731581"},{"key":"S003118202300063X_ref16","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X11000629"},{"key":"S003118202300063X_ref23","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msab120"},{"key":"S003118202300063X_ref14","first-page":"27","article-title":"Genetic and morphological characterization of Thysaniezia tapeworms from cattle and sheep in Senegal","volume":"11","author":"Ndom","year":"2018","journal-title":"Veterinary Parasitology: Regional Studies and Reports"},{"key":"S003118202300063X_ref1","volume-title":"Monographie des cestodes de la famille des Anoplocephalidae","author":"Baer","year":"1927"},{"key":"S003118202300063X_ref5","first-page":"1","article-title":"Anoplocephalid cestodes of veterinary and medical significance: a review","volume":"45","author":"Denegri","year":"1998","journal-title":"Folia Parasitologica"},{"key":"S003118202300063X_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2015.02.008"},{"key":"S003118202300063X_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.11.032"},{"key":"S003118202300063X_ref13","doi-asserted-by":"publisher","DOI":"10.1007\/s11686-022-00607-4"},{"key":"S003118202300063X_ref8","doi-asserted-by":"publisher","DOI":"10.3109\/10520293909110291"},{"key":"S003118202300063X_ref15","first-page":"100337","article-title":"Morphological and genetic characterizations of Avitellina tapeworms from domestic ruminants in Senegal: an evidence of specificity among sheep and cattle host","volume":"18","author":"Ndom","year":"2019","journal-title":"Veterinary Parasitology: Regional Studies and Reports"},{"key":"S003118202300063X_ref2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-010-2060-2"},{"key":"S003118202300063X_ref18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"S003118202300063X_ref19","first-page":"56","article-title":"Studies on the morphology of Moniezia denticulata (Rudolphi, 1810) Blanchard, 1891, with a note on the morphology of Moniezia expansa (Rudolphi, 1810) Blanchard, 1891","volume":"16","author":"Saxena","year":"1964","journal-title":"Indian Journal of Helminthology"},{"key":"S003118202300063X_ref20","first-page":"986","article-title":"Preliminary molecular identification of two helminthes (Moniezia sp. and Paramphistomum sp.) in the province of Taif, Saudi Arabia","volume":"17","author":"Shalaby","year":"2012","journal-title":"World Applied Sciences Journal"},{"key":"S003118202300063X_ref17","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.14-0309"},{"key":"S003118202300063X_ref21","unstructured":"Stiles, CW and Hassall, A (1893) A revision of the adult cestodes of cattle, sheep and allied animals. US Department of Agriculture, Bureau of Animal Industry."},{"key":"S003118202300063X_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2019.101998"},{"key":"S003118202300063X_ref7","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.1983.9693875"},{"key":"S003118202300063X_ref24","first-page":"10","article-title":"Morphological and molecular studies of Moniezia sp. (Cestoda: Anoplocephalidea) a parasite of the domestic goat Capra hircus (L.) in Aurangabad district (MS), India","volume":"8","author":"Thosar","year":"2015","journal-title":"Indian Journal of Applied Research"},{"key":"S003118202300063X_ref3","volume-title":"Helminth Parasites of the Domesticated Animals in India","author":"Bhalerao","year":"1935"},{"key":"S003118202300063X_ref11","doi-asserted-by":"publisher","DOI":"10.5958\/2349-2104.2019.00007.X"},{"key":"S003118202300063X_ref25","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks596"},{"key":"S003118202300063X_ref26","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X17000621"},{"key":"S003118202300063X_ref27","doi-asserted-by":"publisher","DOI":"10.1556\/avet.2013.035"},{"key":"S003118202300063X_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2008.11.009"},{"key":"S003118202300063X_ref10","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2017.12.006"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118202300063X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T10:08:00Z","timestamp":1692353280000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118202300063X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,19]]},"references-count":28,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2023,8]]}},"alternative-id":["S003118202300063X"],"URL":"https:\/\/doi.org\/10.1017\/s003118202300063x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,19]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2023. 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