{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T12:47:11Z","timestamp":1793710031116,"version":"4.6.1"},"reference-count":27,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T00:00:00Z","timestamp":1690761600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Four methods were compared for the diagnosis of human taeniasis caused by <jats:italic>Taenia solium.<\/jats:italic> Fecal samples from persons living in a <jats:italic>T. solium<\/jats:italic> endemic region of Madagascar were examined for taeniid eggs by the Kato\u2013Katz method. Subsequently, samples positive (<jats:italic>n<\/jats:italic> = 16) and negative (<jats:italic>n<\/jats:italic> = 200) for <jats:italic>T. solium<\/jats:italic> eggs were examined by (i) amplification of the fragment of small subunit of the mitochondrial ribosomal RNA (<jats:italic>rrn<\/jats:italic>S) gene using conventional polymerase chain reaction (PCR) and (ii) a nested PCR of a fragment of the <jats:italic>T. solium Tso31<\/jats:italic> gene. Additionally, 12 egg-positive and all egg-negative samples were tested for coproantigen detection. A further 9 egg-positive fecal samples were examined using both PCRs. Of the 12 egg-positive samples tested by PCRs and coproantigen methods, 9 (75%) were positive by <jats:italic>rrn<\/jats:italic>S PCR, 3 (25%) using <jats:italic>Tso31<\/jats:italic>-nested PCR and 9 (75%) by coproantigen testing. None of the 200 egg-negative fecal samples was positive in either <jats:italic>rrn<\/jats:italic>S or <jats:italic>Tso31<\/jats:italic>-nested PCR. Twenty of the 25 egg-positive samples (80%) were positive in <jats:italic>rrn<\/jats:italic>S PCR, and DNA sequencing of PCR amplicons was obtained from 18 samples, all confirmed to be <jats:italic>T. solium<\/jats:italic>. Twelve of the 25 egg-positive samples (48%) were positive in the <jats:italic>Tso31<\/jats:italic>-nested PCR, all of which were also positive by <jats:italic>rrn<\/jats:italic>S PCR. It is suggested that species-specific diagnosis of <jats:italic>T. solium<\/jats:italic> taeniasis may be achieved by either coprological examination to detect eggs or coproantigen testing, followed by <jats:italic>rrn<\/jats:italic>S PCR and DNA sequencing to confirm the tapeworm species in egg-positive or coproantigen-positive samples.<\/jats:p>","DOI":"10.1017\/s0031182023000690","type":"journal-article","created":{"date-parts":[[2023,7,31]],"date-time":"2023-07-31T07:56:50Z","timestamp":1690790210000},"page":"894-900","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of Kato\u2013Katz, PCR and coproantigen for the diagnosis of <i>Taenia solium<\/i> taeniasis"],"prefix":"10.1017","volume":"150","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6655-0086","authenticated-orcid":false,"given":"Marshall W.","family":"Lightowlers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana Edithe Andria","family":"Mananjara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihajamanana","family":"Rakotoarinoro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valisoa C.","family":"Rakotoarison","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Modestine","family":"Raliniaina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harentsoaniaina","family":"Rasamoelina-Andriamanivo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles G.","family":"Gauci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8888-0046","authenticated-orcid":false,"given":"Abdul","family":"Jabbar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kabemba E.","family":"Mwape","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meritxell","family":"Donadeu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noromanana Sylvia","family":"Ramiandrasoa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Alphonse","family":"Nely","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2023,7,31]]},"reference":[{"key":"S0031182023000690_ref5","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00085-19"},{"key":"S0031182023000690_ref10","doi-asserted-by":"publisher","DOI":"10.1645\/GE-938R.1"},{"key":"S0031182023000690_ref16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005743"},{"key":"S0031182023000690_ref12","doi-asserted-by":"publisher","DOI":"10.1111\/pim.12291"},{"key":"S0031182023000690_ref23","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182007002235"},{"key":"S0031182023000690_ref25","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0733-1"},{"key":"S0031182023000690_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2007.07.017"},{"key":"S0031182023000690_ref19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010265"},{"key":"S0031182023000690_ref14","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01172-07"},{"key":"S0031182023000690_ref7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2015.02.011"},{"key":"S0031182023000690_ref1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060686"},{"key":"S0031182023000690_ref24","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.109.080887"},{"key":"S0031182023000690_ref2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000061801"},{"key":"S0031182023000690_ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001594"},{"key":"S0031182023000690_ref26","unstructured":"World Helath Organization (2015) Investing to overcome the global impact of neglected tropical diseases. Third WHO report on neglected tropical diseases. WHO\/HTM\/NTD\/2015.1. 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Retrieved from Research Gate website: https:\/\/www.researchgate.net\/profile\/Richar-Rodriguez-Hidalgo\/publication\/278966245_Improved_PCR-RFLP_assay_for_the_detection_and_differentiation_between_Taenia_solium_and_Taenia_saginata_in_faecal_samples\/links\/5588609f08ae347f9bda9b6f\/Improved-PCR-RFLP-assay-for-the-detection-and-differentiation-between-Taenia-solium-and-Taenia-saginata-in-faecal-samples.pdf (accessed 8 June 2023)."},{"key":"S0031182023000690_ref18","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12089"},{"key":"S0031182023000690_ref20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0008653"},{"key":"S0031182023000690_ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105445"},{"key":"S0031182023000690_ref6","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007408"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182023000690","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:58:25Z","timestamp":1697439505000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182023000690\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,31]]},"references-count":27,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["S0031182023000690"],"URL":"https:\/\/doi.org\/10.1017\/s0031182023000690","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,31]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2023. 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