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The invasive Asian longhorned tick, <jats:italic>Haemaphysalis longicornis<\/jats:italic>, is a known vector of piroplasms in its native range in East Asia and invasive range in Australasia. In the USA, <jats:italic>H. longicornis<\/jats:italic> has been associated with <jats:italic>Theileria orientalis<\/jats:italic> Ikeda outbreaks that caused cattle mortality. To survey invasive populations of <jats:italic>H. longicornis<\/jats:italic> for a broad range of piroplasms, 667 questing <jats:italic>H. longicornis<\/jats:italic> collected in 2021 from 3 sites in New Jersey, USA, were tested with generalist piroplasm primers targeting the 18S small subunit rRNA (395\u2013515 bp, depending on species) and the cytochrome b oxidase loci (1009 bp). Sequences matching <jats:italic>Theileria cervi<\/jats:italic> type F (1 adult, 5 nymphs), an unidentified <jats:italic>Theileria<\/jats:italic> species (in 1 nymph), an undescribed <jats:italic>Babesia<\/jats:italic> sensu stricto (\u2018true\u2019 <jats:italic>Babesia<\/jats:italic>, 2 adults, 2 nymphs), a <jats:italic>Babesia<\/jats:italic> sp. Coco (also a \u2018true <jats:italic>Babesia<\/jats:italic>\u2019, 1 adult, 1 nymph), as well as <jats:italic>Babesia microti<\/jats:italic> S837 (1 adult, 4 nymphs) were recovered. <jats:italic>Babesia microti<\/jats:italic> S837 is closely related to the human pathogen <jats:italic>B. microti<\/jats:italic> US-type. Additionally, a 132 bp sequence matching the cytochrome b locus of deer, <jats:italic>Odocoileus virginanus<\/jats:italic>, was obtained from 2 partially engorged <jats:italic>H. longicornis.<\/jats:italic> The diverse assemblage of piroplasms now associated with <jats:italic>H. longicornis<\/jats:italic> in the USA spans 3 clades in the piroplasm phylogeny and raises concerns of transmission amplification of veterinary pathogens as well as spillover of pathogens from wildlife to humans.<\/jats:p>","DOI":"10.1017\/s0031182023000914","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T09:15:50Z","timestamp":1696410950000},"page":"1063-1069","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":8,"title":["Multiple piroplasm parasites (Apicomplexa: Piroplasmida) in northeastern populations of the invasive Asian longhorned tick, <i>Haemaphysalis longicornis<\/i> Neumann (Ixodida: Ixodidae), in the United 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