{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T10:24:45Z","timestamp":1777976685369,"version":"3.51.4"},"reference-count":21,"publisher":"Cambridge University Press (CUP)","issue":"13","license":[{"start":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:00:00Z","timestamp":1697760000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["232300421313"],"award-info":[{"award-number":["232300421313"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2023,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Blastocystis<\/jats:italic> sp. is a common parasite in the intestinal tract of humans and animals. The clinical diagnosis of <jats:italic>Blastocystis<\/jats:italic> sp. mainly depends on the microscopic observation of parasite, which can lead to false-negative results. An accurate and convenient diagnostic approach for <jats:italic>Blastocystis<\/jats:italic> sp. infection is crucial for effectively preventing and controlling blastocystosis. Herein, we developed a recombinase polymerase amplification (RPA) method for detecting <jats:italic>Blastocystis<\/jats:italic> sp. The results showed that the DNA amplification by RPA established in this study could be performed within 5 min at 37\u00b0C, with maximum band intensity observed at 30 min. The minimum detection limit of RPA was 100 fg <jats:italic>\u03bc<\/jats:italic>L<jats:sup>\u22121<\/jats:sup>, consistent with conventional polymerase chain reaction (cPCR). Furthermore, the RPA method exhibited no cross-reactivity with 7 other non-target pathogens in the intestinal tract. Next, the newly established RPA method was used to analyse 40 fecal samples collected clinically, and the detection results were consistent with cPCR. These results corroborate that the newly developed RPA method has good sensitivity and specificity and offers the advantage of short detection times, which can be harnessed for differential diagnosis and rapid detection of <jats:italic>Blastocystis<\/jats:italic> sp.<\/jats:p>","DOI":"10.1017\/s0031182023000975","type":"journal-article","created":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T08:55:13Z","timestamp":1697792113000},"page":"1221-1225","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Development and application of recombinase polymerase amplification assay for rapid detection of <i>Blastocystis<\/i> sp."],"prefix":"10.1017","volume":"150","author":[{"given":"Xuefang","family":"Mei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changwei","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanrui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luwei","family":"Jia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenke","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowei","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenchao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2916-9147","authenticated-orcid":false,"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"S0031182023000975_ref14","doi-asserted-by":"crossref","first-page":"591","DOI":"10.4269\/ajtmh.15-0276","article-title":"Detection of Entamoeba histolytica by recombinase polymerase amplification","volume":"93","author":"Nair","year":"2015","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182023000975_ref17","doi-asserted-by":"crossref","first-page":"e0004638","DOI":"10.1371\/journal.pntd.0004638","article-title":"An innovative field-applicable molecular test to diagnose cutaneous Leishmania Viannia spp. infections","volume":"10","author":"Saldarriaga","year":"2016","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182023000975_ref18","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.protis.2005.12.001","article-title":"DNA barcoding of Blastocystis","volume":"157","author":"Scicluna","year":"2006","journal-title":"Protist"},{"key":"S0031182023000975_ref13","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1139\/cjm-2021-0329","article-title":"Recombinase polymerase amplification in the molecular diagnosis of microbiological targets and its applications","volume":"68","author":"Mota","year":"2022","journal-title":"Canadian Journal Microbiology"},{"key":"S0031182023000975_ref19","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.1645\/GE-840R.1","article-title":"Detection of Blastocystis hominis in unpreserved stool specimens by using polymerase chain reaction","volume":"92","author":"Stensvold","year":"2006","journal-title":"Journal of Parasitology"},{"key":"S0031182023000975_ref21","first-page":"795","article-title":"Comparison of diagnostic methods (wet mount, trichrome staining, formol-ether, PCR, and xenic in vitro culture) for the detection of Blastocystis in stool samples in Urmia educational hospitals, the Northwest of Iran","volume":"67","author":"Zamani","year":"2021","journal-title":"Annals of Parasitology"},{"key":"S0031182023000975_ref4","doi-asserted-by":"crossref","first-page":"106287","DOI":"10.1016\/j.actatropica.2021.106287","article-title":"Metagenomics applied to the detection of diarrhea 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to diagnose Giardia in stool samples","volume":"92","author":"Crannell","year":"2015","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182023000975_ref2","doi-asserted-by":"crossref","first-page":"187","DOI":"10.7883\/yoken.JJID.2019.054","article-title":"Intestinal protozoan parasitic infections in immunocompromised child patients with diarrhea","volume":"73","author":"Caner","year":"2020","journal-title":"Japanese Journal of Infectious Diseases"},{"key":"S0031182023000975_ref20","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1007\/s00436-003-0995-2","article-title":"Polymerase chain reaction-based genotype classification among human Blastocystis hominis populations isolated from different countries","volume":"92","author":"Yoshikawa","year":"2004","journal-title":"Parasitology 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Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}