{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T11:58:22Z","timestamp":1780401502567,"version":"3.54.1"},"reference-count":31,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:00:00Z","timestamp":1700784000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The genus <jats:italic>Encyclometra<\/jats:italic> is one of the two genera in family Encyclometridae, known for parasitising the oesophagus, stomach and intestine of snakes. Among <jats:italic>Encyclometra<\/jats:italic>, the species present are: <jats:italic>Encyclometra colubrimurorum<\/jats:italic>, <jats:italic>Encyclometra japonica<\/jats:italic>, <jats:italic>Encyclometra asymmetrica<\/jats:italic> and <jats:italic>Encyclometra bungara<\/jats:italic>. Species discrimination within <jats:italic>Encyclometra<\/jats:italic> has predominantly relied on morphological differences, such as the length of the caeca and the position of the testes. Morphological overlaps exist among these species making species discrimination challenging. Additionally, the use of molecular information has been limited for <jats:italic>Encyclometra<\/jats:italic>. To determine the <jats:italic>Encyclometra<\/jats:italic> species infecting <jats:italic>Enhydris enhydris<\/jats:italic> from Thailand and Cambodia, morphological and molecular identification was conducted. Morphological characters and measurements were obtained from 30 <jats:italic>Encyclometra<\/jats:italic> adults, and they were compared with previous studies of other <jats:italic>Encyclometra<\/jats:italic> species. Novel sequences of <jats:italic>E. bungara<\/jats:italic> were generated using the nuclear 18S and 28S ribosomal RNA genes, and the mitochondrial cytochrome c oxidase subunit 1 gene. Our results revealed that the specimens could be morphologically identified as <jats:italic>E. bungara<\/jats:italic>, with support from molecular information obtained from the phylogenies of the 3 genetic markers employed. Molecular analysis showed that the <jats:italic>Encyclometra<\/jats:italic> specimens were distinct from <jats:italic>E. colubrimurorum<\/jats:italic> and <jats:italic>E. japonica<\/jats:italic>. Through morphological and molecular identification of the <jats:italic>Encyclometra<\/jats:italic> specimens found in <jats:italic>E. enhydris<\/jats:italic> from Thailand and Cambodia, we describe and provide a record of <jats:italic>E. bungara<\/jats:italic> in a new host and new locality. Additionally, novel molecular sequences were generated, revealing the phylogenetic position of <jats:italic>E. bungara<\/jats:italic> within the superfamily Gorgoderoidea.<\/jats:p>","DOI":"10.1017\/s0031182023001166","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T09:06:37Z","timestamp":1700816797000},"page":"77-83","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Discovery of <i>Encyclometra bungara<\/i> (Digenea: Encyclometridae) in a new host (<i>Enhydris enhydris<\/i>) from Thailand and Cambodia through morphological and molecular identification"],"prefix":"10.1017","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1821-7801","authenticated-orcid":false,"given":"Abigail Hui En","family":"Chan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0688-2422","authenticated-orcid":false,"given":"Urusa","family":"Thaenkham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akkarin","family":"Poodeepiyasawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9564-7645","authenticated-orcid":false,"given":"Somusa","family":"Boonserm","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1768-4253","authenticated-orcid":false,"given":"Pakteema","family":"Namjad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2963-4811","authenticated-orcid":false,"given":"Panithi","family":"Laoungbua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3448-1132","authenticated-orcid":false,"given":"Tanapong","family":"Tawan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngor Peng","family":"Bun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4894-5148","authenticated-orcid":false,"given":"Napat","family":"Ratnarathorn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6367-6687","authenticated-orcid":false,"given":"Vachirapong","family":"Charoennitiwat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"S0031182023001166_ref1","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1007\/s11230-016-9631-2","article-title":"Molecular approaches to trematode systematics: \u2018best practice\u2019 and implications for future study","volume":"93","author":"Blasco-Costa","year":"2016","journal-title":"Systematic Parasitology"},{"key":"S0031182023001166_ref20","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1645\/22-26","article-title":"Morphologic observation and first molecular characterization of Encyclometra japonica (Digenea: Encyclometridae) in the digestive tract of wild tiger keelbacks (Rhabdophis tigrinus) in Japan","volume":"108","author":"Saito","year":"2022","journal-title":"Journal of Parasitology"},{"key":"S0031182023001166_ref25","doi-asserted-by":"crossref","unstructured":"Thompson, J , Gibson, T and Higgins, D (2002) Multiple sequence alignment using ClustalW and ClustalX. In Current Protocols in Bioinformatics. https:\/\/doi.org\/10.1002\/0471250953.","DOI":"10.1002\/0471250953.bi0203s00"},{"key":"S0031182023001166_ref30","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1017\/S0022149X00019404","article-title":"A review of the trematode genus Encyclometra Baylis and Cannon, 1924","volume":"32","author":"Yeh","year":"1958","journal-title":"Journal of Helminthology"},{"key":"S0031182023001166_ref15","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0065-308X(05)60002-4","article-title":"The use and implications of ribosomal DNA sequencing for the discrimination of Digenea species","volume":"60","author":"Nolan","year":"2005","journal-title":"Advances in Parasitology"},{"key":"S0031182023001166_ref16","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1016\/S0020-7519(03)00049-3","article-title":"Phylogeny and classification of the Digenea (Platyhelminthes: Trematoda)","volume":"33","author":"Olson","year":"2003","journal-title":"International Journal for Parasitology"},{"key":"S0031182023001166_ref17","unstructured":"Rambaut, A (2009) FigTree (Version 1.3.1). Available at http:\/\/tree.bio.ed.ac.uk (accessed August 31, 2023)."},{"key":"S0031182023001166_ref24","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1645\/GE-2764.1","article-title":"Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): a new record of small liver flukes in the greater Mekong sub-region","volume":"97","author":"Thaenkham","year":"2011","journal-title":"The Journal of Parasitology"},{"key":"S0031182023001166_ref26","first-page":"349","volume-title":"Keys to the Trematoda","author":"Tkach","year":"2008"},{"key":"S0031182023001166_ref13","first-page":"129","article-title":"The ecology of the water snakes of Ban Tha Hin, Songkhla Province, Thailand","volume":"47","author":"Murphy","year":"1999","journal-title":"Natural History Bulletin of the Siam Society"},{"key":"S0031182023001166_ref14","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1017\/S003118201000168X","article-title":"Integrating molecular and morphological approaches for characterizing parasite cryptic species: implications for parasitology","volume":"138","author":"Nadler","year":"2011","journal-title":"Parasitology"},{"key":"S0031182023001166_ref4","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1186\/s12864-022-08302-4","article-title":"The potential use of mitochondrial ribosomal genes (12S and 16S) in DNA barcoding and phylogenetic analysis of trematodes","volume":"23","author":"Chan","year":"2022","journal-title":"BMC Genomics"},{"key":"S0031182023001166_ref6","first-page":"179","article-title":"Redescription of Encyclometra colubrimurorum (Rudolphi, 1819) with remarks on the synonymy of E. vitellata Gupta, 1954","volume":"24","author":"Gupta","year":"1977","journal-title":"Folia Parasitologica (Praha)"},{"key":"S0031182023001166_ref29","doi-asserted-by":"crossref","first-page":"265","DOI":"10.2306\/scienceasia1513-1874.1998.24.265","article-title":"Trematodes from amphibians and reptiles of Thailand","volume":"24","author":"Wongsawad","year":"1991","journal-title":"Journal of The Science Society of Thailand"},{"key":"S0031182023001166_ref2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/0166-6851(92)90109-W","article-title":"Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing","volume":"54","author":"Bowles","year":"1992","journal-title":"Molecular and Biochemical Parasitology"},{"key":"S0031182023001166_ref10","first-page":"1","article-title":"Semi-aquatic snake communities of the central plain region of Thailand","volume":"10","author":"Karns","year":"2010b","journal-title":"Tropical Natural History"},{"key":"S0031182023001166_ref12","first-page":"9","article-title":"The rainbow mud snake: Enhydris enhydris (Schneider) [Reptilia: Squamata: Homalopsidae] in Singapore","volume":"2","author":"Lim","year":"2009","journal-title":"Nature in Singapore"},{"key":"S0031182023001166_ref27","doi-asserted-by":"crossref","first-page":"1621","DOI":"10.1080\/00222930110062642","article-title":"The prey and predators of Homalopsinae snakes","volume":"36","author":"Voris","year":"2010","journal-title":"Journal of Natural History"},{"key":"S0031182023001166_ref3","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1186\/s13071-021-04737-y","article-title":"Assessing the suitability of mitochondrial and nuclear DNA genetic markers for molecular systematics and species identification of helminths","volume":"14","author":"Chan","year":"2021","journal-title":"Parasites and Vectors"},{"key":"S0031182023001166_ref7","first-page":"95","article-title":"BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95\/98\/NT","volume":"41","author":"Hall","year":"1999","journal-title":"Nucleic Acids Symposium"},{"key":"S0031182023001166_ref11","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Molecular Biology and Evolution"},{"key":"S0031182023001166_ref18","doi-asserted-by":"crossref","first-page":"2535","DOI":"10.1007\/s00436-014-3903-z","article-title":"Selective and universal primers for trematode barcoding in freshwater snails","volume":"113","author":"Routtu","year":"2014","journal-title":"Parasitology Research"},{"key":"S0031182023001166_ref19","doi-asserted-by":"crossref","DOI":"10.5962\/bhl.title.9157","volume-title":"Entozoorum Synopsis cui Accedunt Mantissa Duplex et Indices Locupletissimi","author":"Rudolphi","year":"1819"},{"key":"S0031182023001166_ref28","first-page":"355","article-title":"Two new trematode parasites of Canton snakes (Trematoda: Lepodermatidae)","volume":"15","author":"Wallace","year":"1936","journal-title":"Lingnan Science Journal"},{"key":"S0031182023001166_ref5","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1645\/GE-2641.1","article-title":"Phylogenetic affinities of Auriculostoma (Digenea: Allocreadiidae), with descriptions of two new species from Peru","volume":"97","author":"Curran","year":"2011","journal-title":"The Journal of Parasitology"},{"key":"S0031182023001166_ref8","first-page":"97","article-title":"The spatial ecology of the rainbow water snake, Enhydris enhydris (Homalopsinae) in southern Thailand","volume":"7","author":"Karns","year":"2000","journal-title":"Herpetological Natural History"},{"key":"S0031182023001166_ref9","doi-asserted-by":"crossref","first-page":"18","DOI":"10.11646\/zootaxa.2452.1.2","article-title":"Phylogeny and biogeography of the Enhydris clade (Serpentes: Homalopsidae)","volume":"2452","author":"Karns","year":"2010a","journal-title":"Zootaxa"},{"key":"S0031182023001166_ref23","first-page":"217","article-title":"Molecular phylogenetic relationship of Paragonimus pseudoheterotremus","volume":"39","author":"Thaenkham","year":"2008","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"S0031182023001166_ref21","first-page":"309","article-title":"Some trematodes of reptiles from Laos","volume":"38","author":"Scholz","year":"1991","journal-title":"Folia Parastiologica"},{"key":"S0031182023001166_ref22","first-page":"132","article-title":"On Encyclometra bungara n. sp. (Trematoda: Plagiorchiidae) from the Indian banded crait, Bungarus fasciatus (Schneider), with new host records of E. japonica Yoshida and Ozaki, 1929","volume":"20","author":"Srivastava","year":"1968","journal-title":"Indian Journal of Helminthology"},{"key":"S0031182023001166_ref31","first-page":"239","article-title":"A new trematode, Encyclometra japonica n. sp. from the snake, Elaphe quadrivirgata","volume":"12","author":"Yoshida","year":"1929","journal-title":"Annotationes Zoologicae Japonenses"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182023001166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T07:37:55Z","timestamp":1707809875000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182023001166\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,24]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["S0031182023001166"],"URL":"https:\/\/doi.org\/10.1017\/s0031182023001166","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,24]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2023. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}