{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T12:47:18Z","timestamp":1765889238801,"version":"3.37.3"},"reference-count":66,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018994","name":"Rajamangala University of Technology Srivijaya","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100018994","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The most common equine tapeworm, <jats:italic>Anoplocephala perfoliata<\/jats:italic>, has often been neglected amongst molecular investigations and has been faced with limited treatment options. However, the recent release of a transcriptome dataset has now provided opportunities for in-depth analysis of <jats:italic>A. perfoliata<\/jats:italic> protein expression. Here, global, and sub-proteomic approaches were utilized to provide a comprehensive characterization of the <jats:italic>A. perfoliata<\/jats:italic> soluble glutathione transferases (GST) (ApGST). Utilizing both bioinformatics and gel-based proteomics, GeLC and 2D-SDS PAGE, the <jats:italic>A. perfoliata \u2018<\/jats:italic>GST-ome\u2019 was observed to be dominated with Mu class GST representatives. In addition, both Sigma and Omega class GSTs were identified, albeit to a lesser extent and absent from affinity chromatography approaches. Moreover, 51 ApGSTs were localized across somatic (47 GSTs), extracellular vesicles (EVs) (Whole: 1 GST, Surface: 2 GSTs) and EV depleted excretory secretory product (ESP) (9 GSTs) proteomes. In related helminths, GSTs have shown promise as novel anthelmintic or vaccine targets for improved helminth control. Thus, provides potential targets for understanding <jats:italic>A. perfoliata<\/jats:italic> novel infection mechanisms, host\u2013parasite relationships and anthelmintic treatments.<\/jats:p>","DOI":"10.1017\/s0031182024000015","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T05:23:08Z","timestamp":1704950588000},"page":"282-294","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":1,"title":["A dominance of Mu class glutathione transferases within the equine tapeworm <i>Anoplocephala perfoliata<\/i>"],"prefix":"10.1017","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6066-6419","authenticated-orcid":false,"given":"Holly M.","family":"Northcote","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boontarikaan","family":"Wititkornkul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Cutress","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan D.","family":"Allen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter M.","family":"Brophy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth E.","family":"Wonfor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0304-4730","authenticated-orcid":false,"given":"Russell 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