{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T21:40:43Z","timestamp":1793828443153,"version":"4.6.1"},"reference-count":23,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["Ricerca corrente Linea 2"],"award-info":[{"award-number":["Ricerca corrente Linea 2"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Some serology assays demonstrated useful for post-treatment monitoring of\n                    <jats:italic>Strongyloides stercoralis<\/jats:italic>\n                    infection. Serology frequently has low specificity, which might be improved by the use of recombinant antigens. The Strongy Detect ELISA is based on 2 recombinant antigens (SsIR and NIE) and proved good accuracy. Aim of this study was to evaluate the performance of this test for the post-treatment monitoring of strongyloidiasis. We tested 38 paired sera, with matched fecal tests results, stored in our biobank and originating from a randomized controlled trial. At baseline, all patients tested positive for at least 1 fecal assay among PCR, direct stool microscopy and agar plate culture. Patients were re-tested with both serology and fecal assays 12 months after treatment. Primary outcome was the relative reduction in optical density (OD) between baseline and follow up. We observed that about 95% samples showed a reduction between pre and post-treatment OD, with a median relative reduction of 93.9% (IQR 77.3%\u201398.1%). In conclusion, the test proved reliable for post-treatment monitoring. However, some technical issues, including that the threshold for positivity has not be predefined, and that a substantial number of samples showed overflow signals, need to be fixed to permit use in routine practice.\n                  <\/jats:p>","DOI":"10.1017\/s0031182024000027","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:01:17Z","timestamp":1704672077000},"page":"295-299","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the SsIR\/NIE recombinant antigen ELISA for the follow up of patients infected by\n                    <i>Strongyloides stercoralis<\/i>\n                    : a diagnostic study"],"prefix":"10.1017","volume":"151","author":[{"given":"Marco","family":"Prato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Tamarozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Tais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Rizzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Mazzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0108-6822","authenticated-orcid":false,"given":"Dora","family":"Buonfrate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"S0031182024000027_ref22","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1128\/JCM.01735-06","article-title":"Use of enzyme-linked immunosorbent assay and dipstick assay for detection of Strongyloides stercoralis infection in humans","volume":"45","author":"van Doorn","year":"2007","journal-title":"Journal of Clinical Microbiology"},{"key":"S0031182024000027_ref6","doi-asserted-by":"crossref","first-page":"1181","DOI":"10.1016\/S1473-3099(19)30289-0","article-title":"Multiple-dose versus single-dose ivermectin for Strongyloides stercoralis infection (Strong Treat 1 to 4): a multicentre, open-label, phase 3, randomised controlled superiority trial","volume":"19","author":"Buonfrate","year":"2019","journal-title":"The Lancet. 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Global Health"},{"key":"S0031182024000027_ref10","doi-asserted-by":"crossref","first-page":"e0005607","DOI":"10.1371\/journal.pntd.0005607","article-title":"Strongyloides seroprevalence before and after an ivermectin mass drug administration in a remote Australian Aboriginal community","volume":"11","author":"Kearns","year":"2017","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182024000027_ref12","doi-asserted-by":"crossref","first-page":"e2165","DOI":"10.1371\/journal.pntd.0002165","article-title":"A public health response against Strongyloides stercoralis: time to look at soil-transmitted helminthiasis in full","volume":"7","author":"Krolewiecki","year":"2013","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182024000027_ref15","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1017\/S0031182016000834","article-title":"Human infection with Strongyloides stercoralis and other related Strongyloides species","volume":"144","author":"Nutman","year":"2017","journal-title":"Parasitology"},{"key":"S0031182024000027_ref18","doi-asserted-by":"crossref","first-page":"e0010126","DOI":"10.1371\/journal.pntd.0010126","article-title":"Strongy detect: preliminary validation of a prototype recombinant Ss-NIE\/Ss-IR based ELISA to detect Strongyloides stercoralis infection","volume":"16","author":"Sears","year":"2022","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182024000027_ref8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/bs.apar.2022.07.001","article-title":"The diagnosis of human and companion animal Strongyloides stercoralis infection: challenges and solutions. A scoping review","volume":"118","author":"Buonfrate","year":"2022","journal-title":"Advances in Parasitology"},{"key":"S0031182024000027_ref13","doi-asserted-by":"crossref","first-page":"749","DOI":"10.4269\/ajtmh.2002.66.749","article-title":"Serology and eosinophil count in the diagnosis and management of strongyloidiasis in a non-endemic area","volume":"66","author":"Loutfy","year":"2002","journal-title":"American Journal of Tropical Medicine and Hygiene"},{"key":"S0031182024000027_ref4","doi-asserted-by":"crossref","first-page":"e0003491","DOI":"10.1371\/journal.pntd.0003491","article-title":"Accuracy of five serologic tests for the follow up of Strongyloides stercoralis infection","volume":"9","author":"Buonfrate","year":"2015","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182024000027_ref7","doi-asserted-by":"crossref","first-page":"468","DOI":"10.3390\/pathogens9060468","article-title":"The global prevalence of Strongyloides stercoralis 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stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina","volume":"17","author":"Krolewiecki","year":"2010","journal-title":"Clinical and Vaccine Immunology"},{"key":"S0031182024000027_ref2","doi-asserted-by":"crossref","first-page":"e2640","DOI":"10.1371\/journal.pntd.0002640","article-title":"Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection","volume":"8","author":"Bisoffi","year":"2014","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182024000027_ref16","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1086\/589718","article-title":"A luciferase immunoprecipitation systems assay enhances the sensitivity and specificity of diagnosis of Strongyloides stercoralis infection","volume":"198","author":"Ramanathan","year":"2008","journal-title":"Journal of Infectious 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\u00a9 The Author(s), 2024. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}