{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T20:23:41Z","timestamp":1776371021454,"version":"3.51.2"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["AMED, grant number: JP23fk0108683"],"award-info":[{"award-number":["AMED, grant number: JP23fk0108683"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Entamoeba moshkovskii<\/jats:italic>, according to recent studies, appears to exert a more significant impact on diarrhoeal infections than previously believed. The efficient identification and genetic characterization of <jats:italic>E. moshkovskii<\/jats:italic> isolates from endemic areas worldwide are crucial for understanding the impact of parasite genomes on amoebic infections. In this study, we employed a multilocus sequence typing system to characterize <jats:italic>E. moshkovskii<\/jats:italic> isolates, with the aim of assessing the role of genetic variation in the pathogenic potential of <jats:italic>E. moshkovskii<\/jats:italic>. We incorporated 3 potential genetic markers: KERP1, a protein rich in lysine and glutamic acid; amoebapore C (apc) and chitinase. Sequencing was attempted for all target loci in 68 positive <jats:italic>E. moshkovskii<\/jats:italic> samples, and successfully sequenced a total of 33 samples for all 3 loci. The analysis revealed 17 distinct genotypes, labelled M1\u2013M17, across the tested samples when combining all loci. Notably, genotype M1 demonstrated a statistically significant association with diarrhoeal incidence within <jats:italic>E. moshkovskii<\/jats:italic> infection (<jats:italic>P<\/jats:italic> = 0.0394). This suggests that M1 may represent a pathogenic strain with the highest potential for causing diarrhoeal symptoms. Additionally, we have identified a few single-nucleotide polymorphisms in the studied loci that can be utilized as genetic markers for recognizing the most potentially pathogenic <jats:italic>E. moshkovskii<\/jats:italic> isolates. In our genetic diversity study, the apc locus demonstrated the highest <jats:italic>H<\/jats:italic><jats:sub>d<\/jats:sub> value and <jats:italic>\u03c0<\/jats:italic> value, indicating its pivotal role in reflecting the evolutionary history and adaptation of the <jats:italic>E. moshkovskii<\/jats:italic> population. Furthermore, analyses of linkage disequilibrium and recombination within the <jats:italic>E. moshkovskii<\/jats:italic> population suggested that the apc locus could play a crucial role in determining the virulence of <jats:italic>E. moshkovskii<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s003118202400026x","type":"journal-article","created":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T08:27:44Z","timestamp":1710145664000},"page":"429-439","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":5,"title":["Genetic characterization of the <i>Entamoeba moshkovskii<\/i> population based on different potential genetic markers"],"prefix":"10.1017","volume":"151","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7815-1226","authenticated-orcid":false,"given":"Sanjib K.","family":"Sardar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ajanta","family":"Ghosal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapas","family":"Haldar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akash","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sweety","family":"Mal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7132-194X","authenticated-orcid":false,"given":"Yumiko","family":"Saito-Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiki","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanta","family":"Dutta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyoshi","family":"Nozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4859-9759","authenticated-orcid":false,"given":"Sandipan","family":"Ganguly","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2024,3,11]]},"reference":[{"key":"S003118202400026X_ref7","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32830ce75f"},{"key":"S003118202400026X_ref15","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2021.102370"},{"key":"S003118202400026X_ref29","doi-asserted-by":"publisher","DOI":"10.1038\/srep01171"},{"key":"S003118202400026X_ref34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00487.x"},{"key":"S003118202400026X_ref5","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2008.79.706"},{"key":"S003118202400026X_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2008.03.013"},{"key":"S003118202400026X_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.12848"},{"key":"S003118202400026X_ref27","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-5-187"},{"key":"S003118202400026X_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2020.111293"},{"key":"S003118202400026X_ref35","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis414"},{"key":"S003118202400026X_ref22","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02301-06"},{"key":"S003118202400026X_ref40","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.678-683.2004"},{"key":"S003118202400026X_ref4","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012001485"},{"key":"S003118202400026X_ref32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0011287"},{"key":"S003118202400026X_ref37","doi-asserted-by":"publisher","DOI":"10.1097\/FPC.0000000000000183"},{"key":"S003118202400026X_ref12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000498"},{"key":"S003118202400026X_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03826.x"},{"key":"S003118202400026X_ref17","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1355.1"},{"key":"S003118202400026X_ref23","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-019-0098-4"},{"key":"S003118202400026X_ref20","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02144-06"},{"key":"S003118202400026X_ref21","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-7-47"},{"key":"S003118202400026X_ref9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4815-4819.2005"},{"key":"S003118202400026X_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(96)02817-4"},{"key":"S003118202400026X_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-015-1084-6"},{"key":"S003118202400026X_ref30","first-page":"202","article-title":"The lysine- and glutamic acid-rich protein KERP1 plays a role in Entamoeba histolytica liver abscess pathogenesis","volume":"10","author":"Santi-Rocca","year":"2008","journal-title":"Cellular Microbiology"},{"key":"S003118202400026X_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2013.11.014"},{"key":"S003118202400026X_ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2003.08.006"},{"key":"S003118202400026X_ref10","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00128-11"},{"key":"S003118202400026X_ref24","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.17.7659"},{"key":"S003118202400026X_ref25","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00013-10"},{"key":"S003118202400026X_ref39","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.issn.0366-6999.20141415"},{"key":"S003118202400026X_ref13","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2022.943445"},{"key":"S003118202400026X_ref2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0905.020548"},{"key":"S003118202400026X_ref26","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00511-21"},{"key":"S003118202400026X_ref28","volume-title":"Amebiasis: Biology and Pathogenesis of Entamoeba","author":"Nozaki","year":"2014"},{"key":"S003118202400026X_ref11","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1959.8.583"},{"key":"S003118202400026X_ref18","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000129"},{"key":"S003118202400026X_ref6","doi-asserted-by":"publisher","DOI":"10.3201\/eid1009.040046"},{"key":"S003118202400026X_ref33","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-023-07952-x"},{"key":"S003118202400026X_ref31","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2022.012"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118202400026X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T03:08:29Z","timestamp":1713755309000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118202400026X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,11]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4]]}},"alternative-id":["S003118202400026X"],"URL":"https:\/\/doi.org\/10.1017\/s003118202400026x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,11]]},"assertion":[{"value":"Copyright \u00a9 The Author(s), 2024. Published by Cambridge University Press","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0\/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}