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However, there is still a lack of evidence regarding the most appropriate methods for this purpose. Here we assessed the diagnostic efficacy of a newly developed serological technique that utilizes the recombinant protein <jats:italic>Sh<\/jats:italic>-TSP-2, applied to the urine and serum of migrants suspected of having urogenital schistosomiasis. The sensitivity, specificity, positive and negative predictive values of an in-house enzyme-linked immunosorbent assay (ELISA) using the recombinant protein <jats:italic>Sh<\/jats:italic>-TSP-2 were analysed and compared with other commercial serological methods. Due to the limitations of microscopy as a perfect reference method, a latent class analysis (LCA) and composite reference standard (CRS) approach was used to determine the sensitivity and specificity of each test. According to the LCA model, the commercial tests NovaLisa<jats:sup>\u00ae<\/jats:sup> and immunochromatography test (ICT) immunoglobulin G\u2013immunoglobulin M (IgG\u2013IgM) presented the highest sensitivity (100%), whereas the <jats:italic>Sh<\/jats:italic>-TSP-2 serum ELISA test had 79.2%. The <jats:italic>Sh<\/jats:italic>-TSP-2 urine and serum ELISA tests had the highest specificities among the serological methods (87.5 and 75%, respectively). CRS modelling showed that the ICT IgG\u2013IgM, NovaLisa<jats:sup>\u00ae<\/jats:sup> and <jats:italic>Sh<\/jats:italic>-TSP-2 serum tests led in sensitivity at 97.1, 88.6 and 71.4%, respectively, with all tests except that the ICT IgG\u2013IgM test having a specificity &gt;90%. <jats:italic>Sh<\/jats:italic>-TSP-2 has been validated as a screening tool for patients suspected of having urogenital schistosomiasis. Although commercial serological tests have shown higher sensitivities, <jats:italic>Sh<\/jats:italic>-TSP-2 could be valuable for confirming results from tests with lower specificity. Nevertheless, further studies with larger patient cohorts are necessary to fully verify its potential.<\/jats:p>","DOI":"10.1017\/s0031182024001574","type":"journal-article","created":{"date-parts":[[2025,1,22]],"date-time":"2025-01-22T08:48:13Z","timestamp":1737535693000},"page":"72-81","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of the recombinant protein <i>Sh<\/i>-TSP-2 for the serological diagnosis of imported urogenital schistosomiasis and comparison with commercially available tests"],"prefix":"10.1017","volume":"152","author":[{"given":"Mar\u00eda Pilar","family":"Luz\u00f3n-Garc\u00eda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Navarro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esther","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel Jes\u00fas","family":"Soriano-P\u00e9rez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"V\u00e1zquez-Villegas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Isabel","family":"Cabeza-Barrera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alex","family":"Loukas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nerea","family":"Castillo-Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Jes\u00fas","family":"Perteguer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1443-7233","authenticated-orcid":false,"given":"Javier","family":"Sotillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4302-7022","authenticated-orcid":false,"given":"Joaqu\u00edn","family":"Salas-Coronas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,1,22]]},"reference":[{"key":"S0031182024001574_ref22","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed8020073"},{"key":"S0031182024001574_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(21)00150-6"},{"key":"S0031182024001574_ref18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003752"},{"key":"S0031182024001574_ref32","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed8010044"},{"key":"S0031182024001574_ref41","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00080-X"},{"key":"S0031182024001574_ref37","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10020205"},{"key":"S0031182024001574_ref43","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2009.01163.x"},{"key":"S0031182024001574_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2016.12.003"},{"key":"S0031182024001574_ref44","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(00)90077-4"},{"key":"S0031182024001574_ref15","first-page":"1","article-title":"Biological agents. 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