{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T09:06:13Z","timestamp":1793610373107,"version":"4.5.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"unspecified","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2025,4]]},"abstract":"Abstract<\/jats:title>The genus Trypanosoma<\/jats:italic> Gruby, 1843 encompasses unique, flagellate haemoparasites infecting all vertebrate classes globally (excluding Antarctica). While trypanosomes in terrestrial mammals are well-studied due to their medical and veterinary significance, those in fishes remain largely unexplored, with limited data on their life cycles and ecological roles. Furthermore, the phylogenetic relationships of numerous aquatic species are unresolved. This gap is notable in South Africa, a region with high marine fish biodiversity, yet only 2 documented marine trypanosome species, 1 in teleosts and 1 in elasmobranchs are known. Our research aims to bridge this knowledge gap for marine fish trypanosomes along South Africa\u2019s southern coast. Blood samples were collected from 246 fishes spanning 23 species at Chintsa East, Tsitsikamma (Garden Route National Park), Boknes, Kariega River Estuary and Groot River West Estuary from 2020 to 2023. Giemsa-stained blood smears were screened for trypanosomes, which were morphologically characterized. Molecular analyses targeting the 18S rRNA gene region were conducted on blood samples positive for trypanosomes. Combined morphological and molecular evidence identified 4 Trypanosoma<\/jats:italic> species: 1 known species, Trypanosoma nudigobii<\/jats:italic> from the klipfish (Clinus superciliosus<\/jats:italic>) and 3 new species: Trypanosoma<\/jats:italic> sp. A from the prison goby (Caffrogobius gilchristi), Trypanosoma bakana<\/jats:italic> n. sp. from the white steenbras (Lithognathus lithognathus<\/jats:italic>) and Trypanosoma bokkom<\/jats:italic> n. sp. from 5 mullet species [the grooved mullet (Chelon dumerili<\/jats:italic>), South African mullet (Chelon richardsonii<\/jats:italic>), striped mullet (Chelon tricuspidens<\/jats:italic>), fl athead grey mullet (Mugil cephalus<\/jats:italic>) and the freshwater mullet (Pseudomyxus capensis<\/jats:italic>)].<\/jats:p><\/jats:sec>","DOI":"10.1017\/s0031182025000496","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T02:51:59Z","timestamp":1744080719000},"page":"531-550","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Molecular and morphological characterization of one known and three new species of fish parasitic Trypanosoma<\/i> Gruby, 1972 from the south coast of South Africa"],"prefix":"10.1017","volume":"152","author":[{"given":"Chandra","family":"Le Roux","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Courtney A.","family":"Cook","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-0586","authenticated-orcid":false,"given":"Edward C.","family":"Netherlands","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marliese","family":"Truter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7950-193X","authenticated-orcid":false,"given":"Nico J.","family":"Smit","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2025,4,8]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025000496","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T08:43:38Z","timestamp":1751445818000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025000496\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["S0031182025000496"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025000496","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0), which permits non-commercial re-use, distribution, and reproduction in any medium, provided that no alterations are made and the original article is properly cited. The written permission of Cambridge University Press must be obtained prior to any commercial use and\/or adaptation of the article.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}