{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T00:09:45Z","timestamp":1771459785443,"version":"3.50.1"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T00:00:00Z","timestamp":1750204800000},"content-version":"unspecified","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"Abstract<\/jats:title>Detection approaches based on environmental DNA (eDNA) are widely used for free-living species but remain underutilized for parasite species. This study applies eDNA detection methods to elucidate the life cycle of the trematode Curtuteria arguinae<\/jats:italic>, which infects the socioeconomically and ecologically important edible cockle (Cerastoderma edule<\/jats:italic>) as its second intermediate host along the northeastern Atlantic coast, including Arcachon Bay, France. The first intermediate and definitive hosts remained unknown. To identify these hosts \u2013 presumed to be a gastropod and a shorebird \u2013 we developed a quantitative PCR (qPCR)-based eDNA approach targeting partial cox1<\/jats:italic> and SSU<\/jats:italic> gene regions of C. arguinae<\/jats:italic>. We tested for C. arguinae<\/jats:italic> eDNA presence in water samples containing separately five dominant gastropod species and fecal samples from known cockle predators, the European oystercatcher (Haematopus ostralegus<\/jats:italic>) and gulls (Larus<\/jats:italic> spp.), collected in Arcachon Bay. C. arguinae<\/jats:italic> eDNA was only detected in water containing the needle snail (Bittium reticulatum<\/jats:italic>), with cercarial emergence confirming infection in 1.6% of individual hosts. Morphological analysis of the cercarial and metacercarial stages revealed variability in collar spine visibility. Additionally, C. arguinae<\/jats:italic> was detected by qPCR in 42% of oystercatcher feces and no gull feces, suggesting oystercatchers are the definitive host. This study is the first to elucidate the complete life cycle of C. arguinae<\/jats:italic>, identifying B. reticulatum<\/jats:italic> as its first intermediate host and H. ostralegus<\/jats:italic> as its definitive host. Our findings highlight the potential of eDNA approaches for resolving parasite life cycles and enabling advances in ecological research on C. arguinae<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182025100267","type":"journal-article","created":{"date-parts":[[2025,6,18]],"date-time":"2025-06-18T08:13:18Z","timestamp":1750234398000},"page":"704-714","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":3,"title":["Elucidation of the life cycle of the trematode Curtuteria arguinae<\/i> (Digenea: Himasthlidae), using environmental DNA detection methods"],"prefix":"10.1017","volume":"152","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-3612-2436","authenticated-orcid":false,"given":"Leslie","family":"Stout","sequence":"first","affiliation":[{"name":"University of Bordeaux"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillemine","family":"Daffe","sequence":"additional","affiliation":[{"name":"University of Bordeaux"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3932-0098","authenticated-orcid":false,"given":"Aur\u00e9lie","family":"Chambouvet","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrien","family":"de Montaudouin","sequence":"additional","affiliation":[{"name":"SEPANSO Aquitaine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Flore","family":"Daramy","sequence":"additional","affiliation":[{"name":"University of Bordeaux"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8012-9423","authenticated-orcid":false,"given":"Xavier","family":"de Montaudouin","sequence":"additional","affiliation":[{"name":"University of Bordeaux"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,6,18]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025100267","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T12:20:51Z","timestamp":1756988451000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025100267\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["S0031182025100267"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025100267","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}