{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T14:30:50Z","timestamp":1767969050082,"version":"3.49.0"},"reference-count":42,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    is a protozoan parasite that causes infection in birds and mammals (terrestrial and marine), both domestic and wild. The state of Tabasco has favourable climatic and ecological conditions for the replication and dissemination of this parasite. Therefore, the aim of this study was to isolate and genotype\n                    <jats:italic>T. gondii<\/jats:italic>\n                    from free-range chickens in this region of M\u00e9xico by PCR-RFLP of 15 genetic markers. A total of 12 chickens were obtained from 7 municipalities. Serological survey by the modified agglutination test (MAT) of chicken serum samples revealed that 9 out of the 12 chickens (75%) tested had antibodies against\n                    <jats:italic>T. gondii<\/jats:italic>\n                    (titres \u2265 1:10).\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    DNA was detected by PCR in tissues from 8 out of the 12 chickens. Twelve viable strains of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    were isolated from the heart and brain samples of eight chickens by bioassay in mice. Genetic characterization of tachyzoite-derived DNA was performed using 10 multilocus RFLP-PCR genotyping markers (\n                    <jats:italic>SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1<\/jats:italic>\n                    and\n                    <jats:italic>Apico<\/jats:italic>\n                    ) and five virulence-related markers (\n                    <jats:italic>CS3, ROP5, ROP16, ROP17<\/jats:italic>\n                    and\n                    <jats:italic>ROP18<\/jats:italic>\n                    ). A total of 6 ToxoDB PCR-RFLP genotypes were identified, including #8 (also known as Type BrIII), #28, #38, and 3 new genotypes designated as #344, #345 and #346. Combination of\n                    <jats:italic>ROP18\/ROP5<\/jats:italic>\n                    alleles were 1\/3, 3\/3 and 4\/3. ToxoDB #344 and #345 genotypes also had a new allele at the\n                    <jats:italic>ROP5<\/jats:italic>\n                    locus. These results suggest high genetic diversity of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    in southeastern M\u00e9xico.\n                  <\/jats:p>","DOI":"10.1017\/s0031182025100401","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T23:30:21Z","timestamp":1751326221000},"page":"1083-1091","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":2,"title":["New genetic variants of\n                    <i>Toxoplasma gondii<\/i>\n                    isolates and a novel\n                    <i>ROP5<\/i>\n                    allele in free-range chickens from Tabasco, M\u00e9xico"],"prefix":"10.1017","volume":"152","author":[{"given":"Luis Fernando","family":"Valenzuela-Moreno","sequence":"first","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Cedillo-Pel\u00e1ez","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Patricia","family":"Rico-Torres","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lluvia Guadalupe","family":"Moreno-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia Virginia","family":"Zaragoza-Vera","sequence":"additional","affiliation":[{"name":"Universidad Ju\u00e1rez Aut\u00f3noma de Tabasco"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz Belinda","family":"Ortiz-Alegr\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irma","family":"Ca\u00f1edo-Solares","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lizbeth","family":"Xicot\u00e9ncatl-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Garc\u00eda-Lacy","sequence":"additional","affiliation":[{"name":"Cirug\u00eda y Zootecnia para \u00c9quidos, FMVZ, UNAM, CDMX"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4269-8251","authenticated-orcid":false,"given":"Heriberto","family":"Caballero-Ortega","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Pediatr\u00eda, CDMX"},{"name":"Universidad Nacional Aut\u00f3noma de M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,7,1]]},"reference":[{"key":"S0031182025100401_ref16","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00170-8"},{"key":"S0031182025100401_ref20","first-page":"191","article-title":"Toxoplasma gondii contamination at an animal agriculture facility: Environmental, agricultural animal, and wildlife contamination indicator evaluation","volume":"16","author":"Kurth","year":"2021","journal-title":"International Journal for Parasitology: Parasites and Wildlife"},{"key":"S0031182025100401_ref41","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/elr028"},{"key":"S0031182025100401_ref36","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991065"},{"key":"S0031182025100401_ref37","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00124-7"},{"key":"S0031182025100401_ref18","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msj030"},{"key":"S0031182025100401_ref29","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2017.11.008"},{"key":"S0031182025100401_ref3","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.868727"},{"key":"S0031182025100401_ref11","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb01426.x"},{"key":"S0031182025100401_ref32","volume-title":"T\u00e9cnicas de Necropsia En Animales Dom\u00e9sticos - 2: edici\u00f3n (Spanish Edition)","author":"Schuneman","year":"2002"},{"key":"S0031182025100401_ref22","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"S0031182025100401_ref23","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.02.007"},{"key":"S0031182025100401_ref39","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2019.12.001"},{"key":"S0031182025100401_ref27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1015980108"},{"key":"S0031182025100401_ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2012.08.004"},{"key":"S0031182025100401_ref40","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2016.04.002"},{"key":"S0031182025100401_ref9","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-4017(97)00135-0"},{"key":"S0031182025100401_ref42","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2019.06.003"},{"key":"S0031182025100401_ref17","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/14.1.68"},{"key":"S0031182025100401_ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.03.006"},{"key":"S0031182025100401_ref19","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-007-0524-9"},{"key":"S0031182025100401_ref5","unstructured":"Cedillo-Pel\u00e1ez, C (2009) Determinaci\u00f3n de genotipos de Toxoplasma gondii en fauna silvestre en M\u00e9xico. MSc Dissertation, Universidad Nacional Aut\u00f3noma de M\u00e9xico."},{"key":"S0031182025100401_ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.12.010"},{"key":"S0031182025100401_ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2007.09.004"},{"key":"S0031182025100401_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2006.08.024"},{"key":"S0031182025100401_ref34","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2015.10.005"},{"key":"S0031182025100401_ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.03.012"},{"key":"S0031182025100401_ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2024.07.009"},{"key":"S0031182025100401_ref1","doi-asserted-by":"publisher","DOI":"10.1177\/014107689008300813"},{"key":"S0031182025100401_ref35","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182013001844"},{"key":"S0031182025100401_ref28","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2023.105473"},{"key":"S0031182025100401_ref4","volume-title":"Primera Edici\u00f3n","author":"Ceballos","year":"2005"},{"key":"S0031182025100401_ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2022.105283"},{"key":"S0031182025100401_ref7","unstructured":"Comisi\u00f3n Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO) y Gobierno del Estado de Tabasco (2021) La biodiversidad en Tabasco: Estudio de Estado. CONABIO 1. Available at: https:\/\/www.biodiversidad.gob.mx\/media\/1\/region\/eeb\/files\/TABASCO_resumen.pdf (accessed 14 May 2025)."},{"key":"S0031182025100401_ref13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0090203"},{"key":"S0031182025100401_ref8","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-815041-2.00003-7"},{"key":"S0031182025100401_ref31","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182024000106"},{"key":"S0031182025100401_ref33","unstructured":"Shwab, EK (2015) Genetic diversity, population structure, and evolution of virulence in Toxoplasma gondii . PhD Dissertation, University of Tennessee."},{"key":"S0031182025100401_ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2014.01.004"},{"key":"S0031182025100401_ref21","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10147"},{"key":"S0031182025100401_ref15","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0012737"},{"key":"S0031182025100401_ref10","volume-title":"Toxoplasmosis of Animals and Humans","author":"Dubey","year":"2010"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025100401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T07:21:03Z","timestamp":1763364063000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025100401\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,1]]},"references-count":42,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S0031182025100401"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025100401","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,1]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}