{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T07:24:19Z","timestamp":1763364259821,"version":"3.45.0"},"reference-count":18,"publisher":"Cambridge University Press (CUP)","issue":"10","license":[{"start":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T00:00:00Z","timestamp":1756944000000},"content-version":"unspecified","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Rosacea is a chronic inflammatory skin disease affecting approximately 5.4% of the world population. Among its pathogenic factors is infestation by\n                    <jats:italic>Demodex<\/jats:italic>\n                    spp. Standardized skin surface biopsy (SSSB) and direct microscopic examination (DME) are widely used methods to measure\n                    <jats:italic>Demodex<\/jats:italic>\n                    spp density (Dd); however, there is no agreement on the method of choice, nor the prevalence of infestation in rosacea patients. This study compared both techniques in rosacea patients. A prospective study was conducted with 61 patients diagnosed with rosacea by dermatologists from two dermatology centres. Dd was evaluated using SSSB and DME in each patient. Results, median sampling time and reported pain were analyzed using appropriate statistical methods. The median Dd was significantly higher with SSSB (11 mites\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) compared to DME (1 mites\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ;\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). Infestation (&gt;5 mites\/cm\n                    <jats:sup>2<\/jats:sup>\n                    ) was detected in 64% of patients with SSSB and in 28% with DME (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). The median sampling time was longer for SSSB (60\u00a0s) than for DME (30\u00a0s;\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00.001). Both methods were associated with mild pain, slightly lower with DME (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.033). SSSB proved more effective than DME for detecting\n                    <jats:italic>Demodex<\/jats:italic>\n                    spp. in rosacea, identifying a greater total number of mites and a higher percentage of infestation. Up to 64% of rosacea patients showed infestation with\n                    <jats:italic>Demodex<\/jats:italic>\n                    spp. using the SSSB technique. The results reinforce the use of SSSB as the standard technique for diagnosing\n                    <jats:italic>Demodex<\/jats:italic>\n                    spp. infestation in rosacea patients.\n                  <\/jats:p>","DOI":"10.1017\/s0031182025100632","type":"journal-article","created":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T10:00:46Z","timestamp":1756980046000},"page":"1064-1069","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Comparison of two techniques for measuring\n                    <i>Demodex folliculorum<\/i>\n                    and\n                    <i>Demodex brevis<\/i>\n                    in rosacea patients: standardized skin surface biopsy vs. direct microscopic examination"],"prefix":"10.1017","volume":"152","author":[{"given":"Jaime","family":"P\u00e9rez Wilson","sequence":"first","affiliation":[{"name":"Cl\u00ednica Alemana-Universidad del Desarrollo, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Andreani Figueroa","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana-Universidad del Desarrollo, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soledad","family":"Aspillaga Vergara","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana-Universidad del Desarrollo, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juana","family":"Benedetto Eblen","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana-Universidad del Desarrollo, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8509-1188","authenticated-orcid":false,"given":"Crist\u00f3bal","family":"Lecaros Cornejo","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana-Universidad del Desarrollo, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Garc\u00eda Ramos","sequence":"additional","affiliation":[{"name":"Medicien, Las Condes"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1196-9422","authenticated-orcid":false,"given":"Diego","family":"M\u00e9ndez Villanueva","sequence":"additional","affiliation":[{"name":"Pontificia Universidad Cat\u00f3lica de Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Vel\u00e1squez Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulina","family":"R\u00edos","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelo","family":"Di Gennaro","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1s","family":"Olivares","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge","family":"Olivares","sequence":"additional","affiliation":[{"name":"Cl\u00ednica Alemana, Vitacura"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,9,4]]},"reference":[{"key":"S0031182025100632_ref1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2010.09645.x"},{"key":"S0031182025100632_ref8","doi-asserted-by":"publisher","DOI":"10.5826\/dpc.1303a182"},{"key":"S0031182025100632_ref16","doi-asserted-by":"publisher","DOI":"10.1111\/srt.12137"},{"key":"S0031182025100632_ref6","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1993.tb00261.x"},{"key":"S0031182025100632_ref15","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-024-03228-1"},{"key":"S0031182025100632_ref14","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13783"},{"key":"S0031182025100632_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.annder.2012.10.592"},{"key":"S0031182025100632_ref5","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-2528"},{"key":"S0031182025100632_ref10","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0000000000000454"},{"key":"S0031182025100632_ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-024-03049-2"},{"key":"S0031182025100632_ref18","doi-asserted-by":"publisher","DOI":"10.5021\/ad.2017.29.2.137"},{"key":"S0031182025100632_ref9","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.12685"},{"key":"S0031182025100632_ref2","doi-asserted-by":"crossref","first-page":"519","DOI":"10.4103\/0378-6323.174423","article-title":"Skin scrapings versus standardized skin surface biopsy to detect Demodex mites in patients with facial erythema of uncertain cause \u2013 A comparative study","volume":"82","author":"Bunyaratavej","year":"2016","journal-title":"Indian Journal of Dermatology, Venereology, and Leprology"},{"key":"S0031182025100632_ref7","first-page":"282","article-title":"Incidence and prevalence of rosacea: A systematic review and meta-analysis","volume":"179","author":"Gether","year":"2018","journal-title":"British Journal of Dermatology"},{"key":"S0031182025100632_ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2017.03.040"},{"key":"S0031182025100632_ref17","doi-asserted-by":"publisher","DOI":"10.1159\/000534245"},{"key":"S0031182025100632_ref3","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12030"},{"key":"S0031182025100632_ref12","doi-asserted-by":"publisher","DOI":"10.1016\/j.piel.2021.01.003"}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025100632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T07:21:04Z","timestamp":1763364064000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025100632\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["S0031182025100632"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025100632","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"type":"print","value":"0031-1820"},{"type":"electronic","value":"1469-8161"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}