{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T08:04:35Z","timestamp":1777363475871,"version":"3.51.4"},"reference-count":37,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Trypanosoma<\/jats:italic>\n                    (\n                    <jats:italic>Megatrypanum<\/jats:italic>\n                    )\n                    <jats:italic>pestanai<\/jats:italic>\n                    is a parasite of the Eurasian badger (\n                    <jats:italic>Meles meles<\/jats:italic>\n                    ), reported in various European countries. However, its presence in the Iberian Peninsula had not been previously investigated. To address this knowledge gap and to assess its occurrence and potential risk factors associated with infection, we analyzed DNA from the spleens of 145 badgers sampled across 4 autonomous regions in northern Spain. Two real-time PCR assays using a reference\n                    <jats:italic>18S rRNA<\/jats:italic>\n                    partial sequence of\n                    <jats:italic>T. pestanai<\/jats:italic>\n                    (92 bp) were developed: one based on SYBR Green chemistry and the other employing a TaqMan probe. Both protocols demonstrated excellent concordance. Defining a sample as positive when at least 1 assay yielded a positive result, the overall prevalence was 35%, consistent with values previously reported in other European populations. A logistic regression model indicated a significantly higher occurrence in badgers from the Eurosiberian bioregion (42%) compared to those from the Mediterranean bioregion (19%). No significant associations were found with age or sex. A subset of positive samples was further analyzed by conventional PCR targeting approximately 900 bp of the\n                    <jats:italic>18S rRNA<\/jats:italic>\n                    gene and sequenced. All 9 high-quality sequences shared 99.75\u2013100% identity with known\n                    <jats:italic>T. pestanai<\/jats:italic>\n                    sequences. These findings confirm that\n                    <jats:italic>T. pestanai<\/jats:italic>\n                    is a common parasite of Iberian badgers and suggest that more humid climatic conditions may favour its persistence, potentially through effects on host ecology or vector dynamics.\n                  <\/jats:p>","DOI":"10.1017\/s0031182025101066","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T06:47:46Z","timestamp":1761547666000},"page":"6-12","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Molecular survey and risk factors of\n                    <i>Trypanosoma pestanai<\/i>\n                    in Eurasian badgers from the northern Iberian Peninsula"],"prefix":"10.1017","volume":"153","author":[{"given":"Mar\u00eda Paz","family":"Peris","sequence":"first","affiliation":[{"name":"Universidad de Zaragoza"},{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, Universidad de Zaragoza-CITA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Rodr\u00edguez-Pastor","sequence":"additional","affiliation":[{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, Universidad de Zaragoza-CITA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Astiz","sequence":"additional","affiliation":[{"name":"Universidad de Zaragoza"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nabil","family":"Halaihel","sequence":"additional","affiliation":[{"name":"Universidad de Zaragoza"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Barral","sequence":"additional","affiliation":[{"name":"NEIKER-Instituto Vasco de Investigaci\u00f3n y Desarrollo Agrario, Basque Research and Technology Alliance (BRTA)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xeider","family":"Gerrikagoitia","sequence":"additional","affiliation":[{"name":"NEIKER-Instituto Vasco de Investigaci\u00f3n y Desarrollo Agrario, Basque Research and Technology Alliance (BRTA)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chabier","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"La Alfranca Wildlife Rescue Center"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferm\u00edn","family":"Urra","sequence":"additional","affiliation":[{"name":"Navarra Environmental Management (Orekan)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roser","family":"Velarde","sequence":"additional","affiliation":[{"name":"Wildlife Ecology & Health group (WE&H) and Servei d\u2019Ecopatologia de Fauna Salvatge (SEFaS)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"Villan\u00faa","sequence":"additional","affiliation":[{"name":"Navarra Environmental Management (Orekan)"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5608-781X","authenticated-orcid":false,"given":"Javier","family":"Mill\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto Agroalimentario de Arag\u00f3n-IA2, Universidad de Zaragoza-CITA"},{"name":"Fundaci\u00f3n ARAID"},{"name":"Universidad Andres Bello"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"S0031182025101066_ref15","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01954-16"},{"key":"S0031182025101066_ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2010.10.003"},{"key":"S0031182025101066_ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-004-1113-9"},{"key":"S0031182025101066_ref12","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020923001987"},{"key":"S0031182025101066_ref21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016977"},{"key":"S0031182025101066_ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2013.12.001"},{"key":"S0031182025101066_ref31","first-page":"308","volume-title":"Atlas y libro rojo de los mam\u00edferos de 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The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives licence (http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0), which permits non-commercial re-use, distribution, and reproduction in any medium, provided that no alterations are made and the original article is properly cited. The written permission of Cambridge University Press must be obtained prior to any commercial use and\/or adaptation of the article.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}