{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T23:31:20Z","timestamp":1773099080271,"version":"3.50.1"},"reference-count":40,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T00:00:00Z","timestamp":1766102400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Freshwater snails are important intermediate hosts for several parasitic diseases, including fascioliosis and schistosomiosis, with significant impacts on human and animal health. In Bangladesh, vector snails have been identified only by morphology. Here, we validate the species of freshwater snails acting as intermediate hosts for\n                    <jats:italic>Fasciola<\/jats:italic>\n                    and\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    spp. using molecular and bioinformatics tools. Following morphology and morphometrics, we identified 9 species of snails:\n                    <jats:italic>Lymnaea auricularia, Lymnaea luteola, Indoplanorbis exustus, Physa acuta, Viviparus bengalensis, Brotia<\/jats:italic>\n                    spp.,\n                    <jats:italic>Thiara<\/jats:italic>\n                    spp. and\n                    <jats:italic>Pila globosa<\/jats:italic>\n                    . Cercarial shedding tests revealed that\n                    <jats:italic>L. auricularia<\/jats:italic>\n                    (0.72%, 7 out of 977) and\n                    <jats:italic>L. luteola<\/jats:italic>\n                    (0.36%, 8 out of 2240) shed fasciolid cercariae, which polymerase chain reaction (PCR) confirmed. But\n                    <jats:italic>I. exustus<\/jats:italic>\n                    (5.43% 19 out of 350),\n                    <jats:italic>L. auricularia<\/jats:italic>\n                    (9.42%, 92 out of 977),\n                    <jats:italic>L. luteola<\/jats:italic>\n                    10.09% (226 out of 2240),\n                    <jats:italic>P. acuta<\/jats:italic>\n                    (2.4%, 11 out of 450) and\n                    <jats:italic>V. bengalensis<\/jats:italic>\n                    (0.14%, 7 out of 500) shed schistosomatid cercariae. The same snail did not shed both fasciolid and schistosomatid cercariae simultaneously. PCR and sequencing of the\n                    <jats:italic>Cox1<\/jats:italic>\n                    gene confirmed the species of the intermediate hosts. The sequences of\n                    <jats:italic>L. auricularia, L. luteola, I. exustus,\u00a0P.\u00a0acuta<\/jats:italic>\n                    and\n                    <jats:italic>V. bengalensis<\/jats:italic>\n                    were identical (99\u221299.7%) to reported sequences of these species. Phylogenetic analysis revealed that sequences of the present study for each species formed well-separated clusters with the corresponding reference sequences. Taken together, the results of this study highlight the importance of molecular tools for confirming snail species and will help target specific vector snails in the particular habitat when designing snail-borne trematode control programs.\n                  <\/jats:p>","DOI":"10.1017\/s0031182025101418","type":"journal-article","created":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T04:29:56Z","timestamp":1766118596000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Ecology and molecular validation of species of vector snails of fascioliosis and schistosomiosis in Bangladesh"],"prefix":"10.1017","author":[{"given":"Md. Haydar","family":"Ali","sequence":"first","affiliation":[{"name":"Bangladesh Agricultural University"},{"name":"Hajee Mohammad Danesh Science and Technology University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayna","family":"Begum","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6176-1036","authenticated-orcid":false,"given":"Md. Shahadat","family":"Hossain","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharmin Shahid","family":"Labony","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umme Razia","family":"Islam","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romana","family":"Parvin","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Mehedi","family":"Hasan","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita Rani","family":"Dey","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Md. Abdul","family":"Alim","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7350-3093","authenticated-orcid":false,"family":"Anisuzzaman","sequence":"additional","affiliation":[{"name":"Bangladesh Agricultural University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2025,12,19]]},"reference":[{"key":"S0031182025101418_ref1","first-page":"1353367","article-title":"Conventional and advanced methods used for the diagnosis of fascioliosis, a food-borne zoonotic disease","author":"Ali","year":"2025","journal-title":"Journal of Parasitology Sesearch"},{"key":"S0031182025101418_ref8","doi-asserted-by":"crossref","DOI":"10.1201\/9781482295184","volume-title":"Freshwater Snails of Africa and their Medical Importance","author":"Brown","year":"1994"},{"key":"S0031182025101418_ref9","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1017\/S0031182000053634","article-title":"Trematodes: Antagonism between species and sterilizing effects on snails in biological control","volume":"84","author":"Combes","year":"1982","journal-title":"Parasitology"},{"key":"S0031182025101418_ref29","doi-asserted-by":"crossref","first-page":"e36","DOI":"10.1017\/S0022149X21000328","article-title":"Do aspermic (parthenogenetic) Fasciola forms have the ability to reproduce their progeny via parthenogenesis?","volume":"95","author":"Ohari","year":"2021","journal-title":"Journal of Helminthology"},{"key":"S0031182025101418_ref36","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: molecular evolutionary genetics analysis version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Molecular Biology and Evolution"},{"key":"S0031182025101418_ref35","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1093\/icb\/32.4.583","article-title":"Interspecific interactions among larval trematode parasites of freshwater and marine snails","volume":"32","author":"Sousa","year":"1992","journal-title":"American Zoologist"},{"key":"S0031182025101418_ref5","doi-asserted-by":"crossref","first-page":"635622","DOI":"10.3389\/fimmu.2021.635622","article-title":"Host-specific serum factors control the development and survival of Schistosoma mansoni","volume":"12","author":"Anisuzzaman","year":"2021","journal-title":"Frontiers in Immunology"},{"key":"S0031182025101418_ref2","doi-asserted-by":"crossref","first-page":"e1476","DOI":"10.1002\/vms3.1476","article-title":"Cholelithiasis in a goat associated with chronic fascioliosis in Bangladesh: A case report and review of literatures","volume":"10","author":"Ali","year":"2024","journal-title":"Veterinary Medicine and Science"},{"key":"S0031182025101418_ref16","doi-asserted-by":"crossref","first-page":"102562","DOI":"10.1016\/j.parint.2022.102562","article-title":"Molecular analyses confirm the coexistence of Fasciola gigantica and parthenogenetic Fasciola in the Philippines","volume":"88","author":"Itagaki","year":"2022b","journal-title":"Parasitology International"},{"key":"S0031182025101418_ref25","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.parint.2018.06.003","article-title":"Positive phototropism is accelerated in Biomphalaria glabrata snails by infection with Schistosoma mansoni","volume":"67","author":"Maeda","year":"2018","journal-title":"Parasitology International"},{"key":"S0031182025101418_ref6","first-page":"87","volume-title":"Advances in Parasitology","author":"Anisuzzaman","year":"2023"},{"key":"S0031182025101418_ref27","volume-title":"Medical and Economic Malacology","author":"Malek","year":"1974"},{"key":"S0031182025101418_ref14","doi-asserted-by":"crossref","first-page":"37","DOI":"10.3329\/jesnr.v5i2.14599","article-title":"Distribution patterns of vector snails and trematode cercaria in their vectors in some selected areas of Mymensingh","volume":"5","author":"Islam","year":"2013","journal-title":"Journal of Environmental Science and Natural Resources"},{"key":"S0031182025101418_ref24","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/s40249-024-01265-6","article-title":"Global, regional and national disease burden of food-borne trematodiases: Projections to 2030 based on the global burden of disease study 2021","volume":"13","author":"Liu","year":"2024","journal-title":"Infectious Diseases of Poverty."},{"key":"S0031182025101418_ref32","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.vetpar.2007.08.006","article-title":"Prevalence of Fasciola hepatica in the intermediate host Lymnaea truncatula detected by real time TaqMan PCR in populations from 70 Swiss farms with cattle husbandry","volume":"150","author":"Schweizer","year":"2007","journal-title":"Veterinary Parasitology"},{"key":"S0031182025101418_ref39","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1079\/PAVSNNR201813062","article-title":"Lymnaeid snails hosts of Fasciola hepatica and Fasciola gigantica (Trematoda: Digenea): A worldwide review","author":"V\u00e1zquez","year":"2019","journal-title":"CABI Reviews"},{"key":"S0031182025101418_ref10","doi-asserted-by":"crossref","first-page":"113","DOI":"10.4003\/006.027.0210","article-title":"Alien non-marine snails and slugs of priority quarantine importance in the United States: A preliminary risk assessment","volume":"27","author":"Cowie","year":"2009","journal-title":"American Malacological Bulletin"},{"key":"S0031182025101418_ref13","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1128\/CMR.00043-14","article-title":"Avian schistosomes and outbreaks of cercarial dermatitis","volume":"28","author":"Hor\u00e1k","year":"2015","journal-title":"Clinical Microbiology Reviews"},{"key":"S0031182025101418_ref18","doi-asserted-by":"crossref","first-page":"2421","DOI":"10.1007\/s00436-013-3464-6","article-title":"The global burden of fasciolosis in domestic animals with an outlook on the contribution of new approaches for diagnosis and control","volume":"112","author":"Khan","year":"2013","journal-title":"Parasitology Research"},{"key":"S0031182025101418_ref28","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1016\/j.micpath.2017.06.006","article-title":"A review on epidemiology, global prevalence and economical losses of fasciolosis in ruminants","volume":"109","author":"Mehmood","year":"2017","journal-title":"Microbial Pathogenesis"},{"key":"S0031182025101418_ref22","article-title":"Mammalian and avian larval schistosomatids in Bangladesh: Molecular characterization, epidemiology, molluscan vectors, and occurrence of human cercarial dermatitis","volume":"11","author":"Labony","year":"2022","journal-title":"Pathogens (Basel, Switzerland)"},{"key":"S0031182025101418_ref11","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1017\/S0022149X17000402","article-title":"A review of bovine fasciolosis and other trematode infections in Nigeria","volume":"92","author":"Elelu","year":"2018","journal-title":"Journal of Helminthology"},{"key":"S0031182025101418_ref7","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1016\/S0065-308X(10)73013-X","article-title":"Studies on the parasitology, phylogeography and the evolution of host-parasite interactions for the snail intermediate hosts of medically important trematode genera in Southeast Asia","volume":"73","author":"Attwood","year":"2010","journal-title":"Advances in Parasitology"},{"key":"S0031182025101418_ref15","doi-asserted-by":"crossref","first-page":"105248","DOI":"10.1016\/j.meegid.2022.105248","article-title":"The causative agents of fascioliasis in animals and humans: Parthenogenetic Fasciola in Asia and other regions","volume":"99","author":"Itagaki","year":"2022a","journal-title":"Infection Genetics and Evolution"},{"key":"S0031182025101418_ref20","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.4102\/ojvr.v89i1.1954","article-title":"Human and animal fasciolosis: Coprological survey in Narok, Baringo and Kisumu counties, Kenya","volume":"89","author":"Kipyegen","year":"2022","journal-title":"The Onderstepoort Journal of Veterinary Research"},{"key":"S0031182025101418_ref21","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1093\/mollus\/53.2.245","article-title":"Indoplanorbis exustus (Deshayes, 1834), a freshwater snail new for Africa, found in Nigeria (Pulmonata: Planorbidae)","volume":"53","author":"Kristensen","year":"1987","journal-title":"Journal of Molluscan Studies"},{"key":"S0031182025101418_ref26","doi-asserted-by":"crossref","first-page":"e63","DOI":"10.1017\/S0022149X19000531","article-title":"Fasciola species and their vertebrate and snail intermediate hosts in East and Southern Africa: A review","volume":"94","author":"Malatji","year":"2019","journal-title":"Journal of Helminthology"},{"key":"S0031182025101418_ref33","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.pt.2017.10.002","article-title":"To reduce the global burden of human schistosomiasis, use \u2018old fashioned\u2019 snail control","volume":"34","author":"Sokolow","year":"2018","journal-title":"Trends in Parasitology"},{"key":"S0031182025101418_ref17","first-page":"3","article-title":"The war against a regional disease in Japan a history of the eradication of Schistosomiasis japonica","volume":"39","author":"Kajihara","year":"2011","journal-title":"Tropical Medicine and Health."},{"key":"S0031182025101418_ref34","doi-asserted-by":"crossref","first-page":"e0004794","DOI":"10.1371\/journal.pntd.0004794","article-title":"Global assessment of schistosomiasis control over the past century shows targeting the snail intermediate host works best","volume":"10","author":"Sokolow","year":"2016","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182025101418_ref38","doi-asserted-by":"crossref","first-page":"1465","DOI":"10.1016\/S0020-7519(97)00183-5","article-title":"From discovery to eradication of schistosomiasis in Japan: 1847\u20131996","volume":"27","author":"Tanaka","year":"1997","journal-title":"International Journal for Parasitology"},{"key":"S0031182025101418_ref30","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1186\/s13071-017-2168-7","article-title":"Fascioliasis risk factors and space-time clusters in domestic ruminants in Bangladesh","volume":"10","author":"Rahman","year":"2017","journal-title":"Parasites and Vectors"},{"key":"S0031182025101418_ref12","doi-asserted-by":"crossref","first-page":"e0006590","DOI":"10.1371\/journal.pntd.0006590","article-title":"A novel cell-free method to culture Schistosoma mansoni from cercariae to juvenile worm stages for in vitro drug testing","volume":"13","author":"Frahm","year":"2019","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182025101418_ref4","first-page":"283","article-title":"Helminth parasites in indigenous ducks: Seasonal dynamics and effects on production performance","volume":"3","author":"Anisuzzaman","year":"2005","journal-title":"Journal of the Bangladesh Agricultural University"},{"key":"S0031182025101418_ref40","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1292\/jvms.17-0308","article-title":"Trematode infections in farm animals and their vector snails in Saint Martin\u2019s Island, the southeastern offshore area of Bangladesh in the Bay of Bengal","volume":"80","author":"Yasin","year":"2018","journal-title":"The Journal of Veterinary Medical Science"},{"key":"S0031182025101418_ref3","first-page":"38","article-title":"Seasonal incidence and the existence of a competition between echinostome and gynaocephalus cercaria in lymnaeid snails","volume":"41","author":"Alim","year":"2007","journal-title":"Bangladesh Veterinary Journal"},{"key":"S0031182025101418_ref19","doi-asserted-by":"crossref","first-page":"e0003657","DOI":"10.1371\/journal.pntd.0003657","article-title":"Historical perspective: Snail control to prevent schistosomiasis","volume":"9","author":"King","year":"2015","journal-title":"PLoS Neglected Tropical Diseases"},{"key":"S0031182025101418_ref23","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.50095","article-title":"Antagonism between parasites within snail hosts impacts the transmission of human schistosomiasis","volume":"8","author":"Laidemitt","year":"2019","journal-title":"eLife"},{"key":"S0031182025101418_ref31","first-page":"100211","article-title":"Phylogeographic genetic variation of Indoplanorbis exustus (Deshayes, 1834) (Gastropoda: Planorbidae) in South and Southeast Asia","volume":"12","author":"Saijuntha","year":"2021","journal-title":"One Health (Amsterdam, Netherlands)"},{"key":"S0031182025101418_ref37","doi-asserted-by":"crossref","first-page":"13","DOI":"10.2147\/RRTM.S397138","article-title":"An update on the pathogenesis of fascioliasis: What do we know?","volume":"15","author":"Tanabe","year":"2024","journal-title":"Research and Reports in Tropical Medicine."}],"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025101418","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T12:57:06Z","timestamp":1773061026000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025101418\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,19]]},"references-count":40,"alternative-id":["S0031182025101418"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025101418","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12,19]]},"assertion":[{"value":"\u00a9 The Author(s), 2025. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}