{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:23:47Z","timestamp":1768314227842,"version":"3.49.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"abstract":"Abstract<\/jats:title>\n \n Ascaridia galli<\/jats:italic>\n and\n Heterakis gallinarum<\/jats:italic>\n , the most prevalent nematodes of chickens, inhabit the small intestine and caeca, respectively, and often co-occur. Current excreta egg count (EEC) methods do not differentiate between their eggs, and although chickens produce two distinct excreta types \u2013 intestinal excreta (IE) and caecal excreta (CE) \u2013 the distribution of eggs of these species across them remains poorly understood. Forty Hy-Line Brown laying hens (40 weeks, mean body weight (BW) 2\u00b707\u00a0\u00b1\u00a00\u00b702\u00a0kg), cleared of prior nematode infection and artificially infected with\n A. galli<\/jats:italic>\n (n\u00a0=\u00a020) or\n H. gallinarum<\/jats:italic>\n (n\u00a0=\u00a020) were housed in separate floor pens and monitored for 26\u00a0weeks. Assessments included clinical signs, EECs from IE, CE and mixed excreta (ME), and worm recovery from subsets of birds at 8, 14, 20 and 26\u00a0weeks. Neither infection resulted in clinical signs, but\n A. galli<\/jats:italic>\n slightly reduced BW gain (0\u00b75\u00a0g\/week\/hen) than\n H. gallinarum<\/jats:italic>\n (2\u00b78\u00a0g\/week\/hen). Egg detection aligned with worm predilection sites:\n A. galli<\/jats:italic>\n eggs were predominantly found in IE, while\n H. gallinarum<\/jats:italic>\n eggs were largely confined to CE. In ME samples, egg counts were reduced by 45% relative to IE for\n A. galli<\/jats:italic>\n and 60% relative to CE for\n H. gallinarum<\/jats:italic>\n . EECs showed a negative but non-significant association with excreta moisture content. Natural re-infection produced a stable\u00a0adult worm population in both infections. These findings demonstrate that analysing IE and CE separately provides a practical, non-lethal approach for differentiating these infections, while ME appears to have limited diagnostic utility. Further studies should evaluate these patterns across broader conditions and individual variation.\n <\/jats:p>","DOI":"10.1017\/s0031182025101534","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T08:54:45Z","timestamp":1767776085000},"page":"1-11","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Differential detection of\n Ascaridia galli<\/i>\n and\n Heterakis gallinarum<\/i>\n eggs in intestinal and caecal excreta of floor-housed laying hens: a longitudinal study"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3777-8257","authenticated-orcid":false,"given":"Teka","family":"Feyera","sequence":"first","affiliation":[{"name":"University of New England"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan","family":"Sharpe","sequence":"additional","affiliation":[{"name":"New South Wales Department of Primary Industries and Regional Development"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Ruhnke","sequence":"additional","affiliation":[{"name":"Freie Universit\u00e4t Berlin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen W.","family":"Walkden-Brown","sequence":"additional","affiliation":[{"name":"University of New England"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[2026,1,7]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182025101534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T09:08:51Z","timestamp":1768295331000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182025101534\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,7]]},"references-count":0,"alternative-id":["S0031182025101534"],"URL":"https:\/\/doi.org\/10.1017\/s0031182025101534","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,7]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}