{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:16:44Z","timestamp":1785403004110,"version":"3.56.0"},"reference-count":46,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T00:00:00Z","timestamp":1770595200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Strongyloides stercoralis<\/jats:italic>\n                    is an intestinal nematode capable of infecting humans and dogs. Occurrence in dogs from temperate, traditionally non-endemic regions remains poorly characterized, often due to the absence of accessible veterinary diagnostic tests. Recent reports of infections from temperate metropolitan areas have raised concerns about the extent of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    prevalence in dogs in these regions. This study investigated the presence of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    DNA in canine faecal samples from Sydney, New South Wales, Australia, using a real-time quantitative PCR (qPCR) targeting the 18S rRNA gene, with a limit of detection of 2 DNA copies. Archived faecal DNA (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0448) collected between 2014 and 2024 from 2 university veterinary hospitals were screened. Of all samples, 1 (0.02%) was positive for\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    DNA, corresponding to approximately 6.8\u00a0\u00d7\u00a010\n                    <jats:sup>3<\/jats:sup>\n                    18S rDNA copies, equivalent to 3.2 of\n                    <jats:italic>S. ratti<\/jats:italic>\n                    third-stage larvae, per 250\u00a0mg of dog faeces. Partial\n                    <jats:italic>cox1<\/jats:italic>\n                    and 18S rDNA loci (HVR-I and HVR-IV) deep amplicon sequencing confirmed that\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    circulates between dogs and humans. The positive sample originated from a Border Collie puppy presenting with gastrointestinal signs. Although detection was rare, this confirms the parasite\u2019s presence in companion dogs within a temperate urban environment. The results highlight the diagnostic utility of 18S rDNA-based qPCR for retrospective surveillance and support the inclusion of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    in molecular diagnostic panels for dogs with gastrointestinal disease. Expanded, targeted molecular and coproscopic surveillance is warranted to clarify the prevalence, distribution and zoonotic potential of\n                    <jats:italic>S. stercoralis<\/jats:italic>\n                    in dogs across Australia\u2019s non-endemic regions.\n                  <\/jats:p>","DOI":"10.1017\/s0031182026101693","type":"journal-article","created":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T06:10:54Z","timestamp":1770617454000},"page":"531-539","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Retrospective screening reveals the rare occurrence of zoonotic\n                    <i>Strongyloides stercoralis<\/i>\n                    in dogs from temperate Australia, 2014\u20132024"],"prefix":"10.1017","volume":"153","author":[{"given":"Jaisy","family":"Chong","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1459-9117","authenticated-orcid":false,"given":"Jan","family":"\u0160lapeta","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"},{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maira Nascimento","family":"Meggiolaro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Green","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8364-9515","authenticated-orcid":false,"given":"Rogan","family":"Lee","sequence":"additional","affiliation":[{"name":"Level 3 Institute of Clinical Pathology and Medical Research (ICPMR)"},{"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Ward","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolle","family":"Kirkwood","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0384j8v12","id-type":"ROR","asserted-by":"publisher"}],"name":"The University of Sydney"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,2,9]]},"reference":[{"key":"S0031182026101693_ref13","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-023-05763-8"},{"key":"S0031182026101693_ref32","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05049-x"},{"key":"S0031182026101693_ref41","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182016001116"},{"key":"S0031182026101693_ref8","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00033-23"},{"key":"S0031182026101693_ref22","doi-asserted-by":"publisher","DOI":"10.16886\/IAS.2016.04"},{"key":"S0031182026101693_ref37","first-page":"89","article-title":"Strongyloides stercoralis in Swiss dogs - 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Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}