{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:31:05Z","timestamp":1785403865426,"version":"3.56.0"},"reference-count":84,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T00:00:00Z","timestamp":1780358400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Gastrointestinal protist (GIP) infections, mainly caused by\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    and\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp., are major contributors to childhood morbidity in resource-limited settings, yet their epidemiology in Madagascar remains poorly documented. We conducted a microscopy-based prospective cross-sectional study in 3 deprived areas of southern Madagascar to investigate pathogenic and commensal protists among children and adolescents (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a0318), young women (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a057) and their children (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a061). Epidemiological questionnaires were collected, and selected species were characterized using PCR and Sanger sequencing. Risk factors were analysed using binary logistic regression. A total of 436 stool samples and 221 questionnaires were analysed.\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    was the most prevalent pathogenic species (81.9%), followed by\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp. (1.2%).\n                    <jats:italic>Balantioides coli<\/jats:italic>\n                    was not detected. Potentially pathogenic species included\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. (70.4%) and\n                    <jats:italic>Dientamoeba fragilis<\/jats:italic>\n                    (0.2%). The most frequent commensals were\n                    <jats:italic>Entamoeba coli<\/jats:italic>\n                    (37.4%),\n                    <jats:italic>Endolimax nana<\/jats:italic>\n                    (15.4%) and\n                    <jats:italic>Iodamoeba b\u00fctschlii<\/jats:italic>\n                    (12.6%). Assemblages A, B and A\u00a0+\u00a0B were identified for\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    , and\n                    <jats:italic>C. hominis, C. felis<\/jats:italic>\n                    and\n                    <jats:italic>C. parvum<\/jats:italic>\n                    for\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp.;\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    subtypes ST1\u2013ST3 were detected.\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    infection was associated with abdominal pain and loss of appetite, but not diarrhoea. Geographical origin predicted\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    infection and\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. and\n                    <jats:italic>E. coli<\/jats:italic>\n                    colonization. GIP infections by\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    and, to a lesser extent, by\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    spp., are a public health concern in resource-limited settings in Madagascar. Our results are the most important contribution made in the country in terms of molecular epidemiology of intestinal protist.\n                  <\/jats:p>","DOI":"10.1017\/s0031182026102200","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:09:13Z","timestamp":1780380553000},"page":"1-14","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and genetic diversity of intestinal protists in vulnerable populations of southern Madagascar"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3170-1008","authenticated-orcid":false,"given":"Gabriela","family":"Tapia-Veloz","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/043nxc105","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Valencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela 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