{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:04:18Z","timestamp":1786122258359,"version":"build-2736575974"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","license":[{"start":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:00:00Z","timestamp":1785110400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["cambridge.org"],"crossmark-restriction":true},"short-container-title":["Parasitology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Schistosomiasis remains a major public health concern in Madagascar, where access to sensitive and user-friendly diagnostic tools is essential for effective control. This study evaluated the diagnostic performance of the\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP assay for detecting\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    spp. in stool samples from schoolchildren in a rural area of central Madagascar, comparing it with Kato-Katz and a commercial qPCR assay. A total of 102 children aged 5\u201315\u00a0years from Soavinarivo participated. Stool samples were analysed using Kato-Katz, qPCR and\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP. All molecular analyses were performed in a well-equipped laboratory in Spain. Agreement between methods was assessed using the Kappa coefficient and McNemar\u2019s test, and diagnostic accuracy was calculated using a composite reference standard. Molecular methods detected the highest prevalence of infection. Agreement between Kato-Katz and molecular methods was low (Kappa\u00a0=\u00a00.259 for qPCR; 0.347 for\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP). Moderate-intensity infections were most frequent (23\/40), all detected by qPCR (median Ct: 26) and 20\/23 by\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP. Light-intensity infections accounted for 16\/40; qPCR detected 11\/16 (median Ct: 28) and\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP 13\/16. qPCR showed the highest diagnostic performance (sensitivity 92.2%, negative predictive value 80.6%, accuracy 94.1%), followed by\n                    <jats:italic>Schisto-<\/jats:italic>\n                    LAMP (sensitivity 81.8%, negative predictive value 64.1%, accuracy 86.3%), with Kato-Katz lowest.\n                    <jats:italic>Schisto<\/jats:italic>\n                    -LAMP outperforms conventional microscopy and provides diagnostic performance close to that of qPCR. Given its simplicity, speed, and minimal equipment requirements, it could be applied in small laboratories in endemic, resource-limited settings, such as rural areas of Madagascar.\n                  <\/jats:p>","DOI":"10.1017\/s0031182026102534","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T01:58:19Z","timestamp":1785117499000},"page":"1-7","update-policy":"https:\/\/doi.org\/10.1017\/policypage","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of a\n                    <i>Schisto<\/i>\n                    -LAMP assay for the molecular diagnosis of schistosomiasis: a proof-of-principle comparative study with qPCR and Kato-Katz"],"prefix":"10.1017","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3170-1008","authenticated-orcid":false,"given":"Gabriela","family":"Tapia-Veloz","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/043nxc105","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Valencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6299-7665","authenticated-orcid":false,"given":"M\u00f3nica","family":"Gozalbo","sequence":"additional","affiliation":[{"name":"University of Valencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e0rius V.","family":"Fuentes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/043nxc105","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Valencia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"Crego-Vicente","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02f40zc51","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Salamanca"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danielle Aurore Doll","family":"Rakoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02w4gwv87","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Antananarivo"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5977-3337","authenticated-orcid":false,"given":"Maria","family":"Trelis","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/043nxc105","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Valencia"},{"id":[{"id":"https:\/\/ror.org\/05n7v5997","id-type":"ROR","asserted-by":"publisher"}],"name":"Health Research Institute La Fe"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Fern\u00e1ndez-Soto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02f40zc51","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Salamanca"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[2026,7,27]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182026102534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T09:31:21Z","timestamp":1786095081000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182026102534\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,27]]},"references-count":0,"alternative-id":["S0031182026102534"],"URL":"https:\/\/doi.org\/10.1017\/s0031182026102534","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,27]]},"assertion":[{"value":"\u00a9 The Author(s), 2026. Published by Cambridge University Press.","name":"copyright","label":"Copyright","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http:\/\/creativecommons.org\/licenses\/by\/4.0), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited.","name":"license","label":"License","group":{"name":"copyright_and_licensing","label":"Copyright and Licensing"}},{"value":"This content has been made available to all.","name":"free","label":"Free to read"}]}}