{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T04:51:33Z","timestamp":1764996693506},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1997,3,1]],"date-time":"1997-03-01T00:00:00Z","timestamp":857174400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,3]]},"abstract":"<jats:p>When crude extracts of <jats:italic>Spirometra mansoni<\/jats:italic> plerocercoid\n \n\n(sparganum) were analysed by SDS\u2013polyacrylamide gel electrophoresis\n \n\n(PAGE)\/immunoblot using patients' sera, IgE antibodies \n\nreacted specifically with 21, 27 and 53 kDa proteins. \n\nThe 21 and 27 kDa proteins have been previously characterized as \n\ncysteine proteases. In this study, the 53 kDa protein \n\nwas confirmed, by immunoprecipitation, to induce a specific IgE \n\nresponse. The protein was purified by affinity chromatography using an\n IgG1 \n\n(\u03ba2) type mAb. The protein was partially sensitive to \n\npeptide-<jats:italic>N<\/jats:italic><jats:sup>4<\/jats:sup>-(<jats:italic>N<\/jats:italic>-acetyl-\u03b2-glucosaminyl)asparagine\n \n\namidase F (endo F) digestion. It exhibited an endoproteinase activity in\n a \n\nthiol-dependent manner preferentially degrading \n\nbenzoyloxycarboxyl-phenylalanyl-arginyl-4-methoxy-\u03b2-naphthylamide\n \n\n(Z-phe-arg-MNA) of a panel of substrates tested. This endoprotease activity\n was \n\nmaximal at pH 6\u00b75 and in 0\u00b71 <jats:sc>M<\/jats:sc> sodium phosphate.\n \n\nThe proteolytic activity was inhibited by 10<jats:sup>\u22125<\/jats:sup><jats:sc>M<\/jats:sc><jats:sc>L<\/jats:sc>-trans-epoxysuccinyl-<jats:sc>L<\/jats:sc>-leucylamido-(4-guanidino)butane\n (E-64) and \n\n1 m<jats:sc>M<\/jats:sc> iodoacetamide (IAA), and potentiated by dithiothreitol \n\n(DTT, 5 m<jats:sc>M<\/jats:sc>).<\/jats:p>","DOI":"10.1017\/s0031182096008529","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"263-271","source":"Crossref","is-referenced-by-count":20,"title":["A neutral cysteine protease of <i>Spirometra mansoni<\/i> \n\nplerocercoid invoking an IgE response"],"prefix":"10.1017","volume":"114","author":[{"given":"Y.","family":"KONG","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.-Y.","family":"KANG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. H.","family":"KIM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.-B.","family":"CHUNG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.-Y.","family":"CHO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,3,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182096008529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,7]],"date-time":"2019-04-07T19:19:53Z","timestamp":1554664793000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182096008529\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,3]]}},"alternative-id":["S0031182096008529"],"URL":"https:\/\/doi.org\/10.1017\/s0031182096008529","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,3]]}}}