{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T17:40:54Z","timestamp":1777484454124,"version":"3.51.4"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[1997,4,1]],"date-time":"1997-04-01T00:00:00Z","timestamp":859852800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,4]]},"abstract":"<jats:p>Part of the C\u03b53\u2013C\u03b54 region of the ovine immunoglobulin\n E \n(IgE) gene (nucleotides 1111\u20131575) was amplified by PCR. \nThe recombinant protein (recIgE1-2) was expressed in <jats:italic>E. coli<\/jats:italic> and\n both \nmonoclonal and polyclonal antibodies were \nproduced. These antibodies recognized recIgE1-2 and native IgE on Western\n blots \nand in ELISA. The polyclonal serum \nshowed cross-reactivity with other sheep immunoglobulin classes. The monoclonal \nantibody was specific for ovine IgE \nand goat IgE. Infection of sheep with the abomasal nematode <jats:italic>Haemonchus \ncontortus<\/jats:italic> resulted in elevated IgE levels in \nserum 2\u20134 weeks after infection, as measured by sandwich ELISA using\n the \nrabbit polyclonal as capture antibody and \nthe monoclonal antibody against ovine IgE as second antibody. A negative \ncorrelation between worm counts and total \nserum IgE levels at the end of the experiment was found in repeatedly infected \nsheep. Significant increased levels of \nexcretory\u2013secretory antigens specific IgE levels were found after\n <jats:italic>H. \ncontortus<\/jats:italic> infection. In contrast, no significant changes \nin 3rd-stage larvae (L3) antigen-specific IgE titre in sera could be detected \nafter infection.<\/jats:p>","DOI":"10.1017\/s0031182096008633","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"395-406","source":"Crossref","is-referenced-by-count":67,"title":["Production of a monoclonal antibody specific for ovine \nimmunoglobulin E and its application to monitor serum \nIgE responses to <i>Haemonchus contortus<\/i> infection"],"prefix":"10.1017","volume":"114","author":[{"given":"F. N. J.","family":"KOOYMAN","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J. S.","family":"VAN KOOTEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. F.","family":"HUNTLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"MacKELLAR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. W. C. A.","family":"CORNELISSEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. D. F. H.","family":"SCHALLIG","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,4,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182096008633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T21:08:41Z","timestamp":1557608921000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182096008633\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,4]]}},"alternative-id":["S0031182096008633"],"URL":"https:\/\/doi.org\/10.1017\/s0031182096008633","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,4]]}}}