{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:28:51Z","timestamp":1792740531655,"version":"4.5.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[1997,7,1]],"date-time":"1997-07-01T00:00:00Z","timestamp":867715200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,7]]},"abstract":"<jats:p>A novel method for the isolation of schistosome eggs and miracidia\n \n\nfrom livers of mice infected with <jats:italic>Schistosoma japonicum<\/jats:italic> \n\nor <jats:italic>S. mansoni<\/jats:italic> is described. The method employed collagenase B\n to \n\ndegrade the interstitial matrix of mouse liver tissue, \n\nafter which the schistosome eggs were separated from the liver cells by\n 2 \n\nsingle-step density centrifugations through \n\nPercoll. Using this procedure sufficient quantities of miracidia were \n\nobtained to generate a cDNA library. Southern blot \n\nanalysis demonstrated that miracidia isolated by this method were free\n \n\nfrom contaminating host DNA.<\/jats:p>","DOI":"10.1017\/s0031182097001091","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"29-32","source":"Crossref","is-referenced-by-count":122,"title":["A method for the isolation of schistosome eggs and \n\nmiracidia free of contaminating host tissues"],"prefix":"10.1017","volume":"115","author":[{"given":"J. P.","family":"DALTON","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. R.","family":"DAY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"DREW","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. J.","family":"BRINDLEY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1997,7,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097001091","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T16:36:13Z","timestamp":1557592573000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097001091\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7]]}},"alternative-id":["S0031182097001091"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097001091","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7]]}}}