{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:05:36Z","timestamp":1791291936787,"version":"4.1.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1997,8,1]],"date-time":"1997-08-01T00:00:00Z","timestamp":870393600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p>The taxonomic situation in the genus <jats:italic>Tritrichomonas<\/jats:italic> is\n the \nsubject of controversial discussion: potentially <jats:italic>T. foetus<\/jats:italic> and \n<jats:italic>T. suis<\/jats:italic>, the tritrichomonads from cattle and swine, respectively, \ncould belong to the same species. In order to shed some \nlight on this question, a molecular biological analysis was performed.\n The \n5\u00b78S rRNA gene and the flanking internal \ntranscribed spacer regions (ITS1 and ITS2) of 12 different isolates of\n 3 \n<jats:italic>Tritrichomonas<\/jats:italic> species <jats:italic>T. foetus<\/jats:italic>, <jats:italic>T. suis<\/jats:italic> and\n <jats:italic>T. \nmobilensis<\/jats:italic> were enzymatically amplified by PCR and subcloned. Also,\n the \ncorresponding regions of the trichomonads \n<jats:italic>Trichomonas vaginalis<\/jats:italic>, <jats:italic>T. tenax<\/jats:italic>, <jats:italic>T. gallinae<\/jats:italic>\n and \n<jats:italic>Pentatrichomonas hominis<\/jats:italic> were included in this study. Sequence\n analysis \nof cloned fragments was used to compare the parasite isolates. The genus \n<jats:italic>Tritrichomonas<\/jats:italic> exhibited an extremely high \ndegree of homogeneity. All <jats:italic>T. foetus<\/jats:italic> and <jats:italic>T. suis<\/jats:italic> isolates \nhad identical sequences, and only 1 substitution was found in the \nITS2 region of <jats:italic>T. mobilensis<\/jats:italic>. In contrast, the genus <jats:italic>Trichomonas<\/jats:italic> \nshared more diversity. The results obtained in this study \nsupport a possible future revision of the taxonomic classification of \ntritrichomonads.<\/jats:p>","DOI":"10.1017\/s0031182097001212","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"111-119","source":"Crossref","is-referenced-by-count":191,"title":["Comparative sequence analysis of 5\u00b78S rRNA genes and \ninternal transcribed spacer (ITS) regions of trichomonadid protozoa"],"prefix":"10.1017","volume":"115","author":[{"given":"R. S. J.","family":"FELLEISEN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1997,8,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097001212","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T20:29:56Z","timestamp":1557606596000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097001212\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8]]}},"alternative-id":["S0031182097001212"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097001212","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8]]}}}