{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T19:20:32Z","timestamp":1700162432697},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1997,8,1]],"date-time":"1997-08-01T00:00:00Z","timestamp":870393600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,8]]},"abstract":"<jats:p>A tegumental vesicle preparation from adult male <jats:italic>Schistosoma \nmansoni<\/jats:italic> was developed that allows the resolution of single \nion-channel currents. Adult male schistosomes were exposed to a low pH \n(3\u00b775) medium for a period of approximately \n30 min at 37\u00b0C. During this period smooth vesicles formed from the tegument. \nFluorescence microscopy following staining of the tegument with the dye, \n5-<jats:italic>N<\/jats:italic>-[octadecanoyl]aminofluorescein (AF-18), transmission \nelectron microscopy \nand scanning electron microscopy revealed that the vesicles were produced\n from the \nouter tegumental membrane. The \nfluorescence studies showed the presence of the double bilayer structure\n of the outer \nmembrane in &gt;41% of the vesicles. \nThese studies suggested that the preparation is suitable for single-channel \nrecording with the patch-clamp technique. \nCell-attached and isolated inside-out patch recordings of ion-channel \nactivity were obtained with giga-ohm resistance \nseals. Different types of ion-channel were recorded from tegumental vesicles \nfrom male schistosomes, illustrating the \npotential of the technique. The channels observed included: a non-selective \ncation channel (360 pS); a K<jats:sup>+<\/jats:sup> channel (with \na conductance of 115 pS in high bath-K conditions); and a Cl<jats:sup>\u2212<\/jats:sup> \nselective channel (20 pS). The currents of these ion-channels may cross \nthe double bilayer of the outer tegumental membrane.<\/jats:p>","DOI":"10.1017\/s0031182097001273","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"183-192","source":"Crossref","is-referenced-by-count":7,"title":["A vesicle preparation for resolving single-channel currents \nin tegument of male <i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"115","author":[{"given":"A. P.","family":"ROBERTSON","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"MARTIN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"KUSEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,8,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097001273","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T20:29:59Z","timestamp":1557606599000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097001273\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,8]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,8]]}},"alternative-id":["S0031182097001273"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097001273","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,8]]}}}