{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,28]],"date-time":"2025-08-28T12:46:18Z","timestamp":1756385178767},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>A cationic <jats:italic>Schistosoma mansoni<\/jats:italic> cercarial antigen was shown\n to \nbe a serine protease as it was capable of hydrolysing \n<jats:italic>N<\/jats:italic>-acetyl-<jats:sc>dl<\/jats:sc>-phenylalanine \u03b2-naphthyl ester (NAPBNE) \nafter precipitation by immunoelectrophoresis, and this reaction was \nmodulated by the serine protease inhibitors phenylmethanesulfonyl fluoride \n(PMSF) and diisopropylfluorophosphate \n(DFP). The antigen in the immunoprecipitin arcs could also be radio-isotope \nlabelled with tritiated DFP. The peptidolytic \nenzyme identified in immunoelectrophoresis with polyspecific \nsera and radio-isotope labelled with tritiated DFP had a \nrelative molecular size of approximately 27 kDa in sodium dodecyl \nsulphate polyacrylamide gel electrophoresis (SDS\u2013PAGE), \nand evidence obtained after partial purification, SDS\u2013PAGE \nand immunoblotting supported this size estimate \nfor the enzyme. A rabbit antiserum raised against the peptidolytic \nantigen reacted against a doublet of antigens at \n27\/28 kDa in immunoelectrophoresis arcs and against an antigen of \n60 kDa in Western immunoblots of crude cercarial \nhomogenate. However, the latter serum precipitated the cationic antigen \nin immunoelectrophoresed cercarial homogenates \nonly after pre-incubation of the homogenates with PMSF. Fractions containing \nthe partially purified protease also \ndegraded radio-isotope labelled human IgG. The reactivity of a range of \npolyspecific and monospecific rabbit antisera in \nWestern blots with larval extracts indicated that antibody responses \nagainst the 27\/28 kDa doublet may be modulated. \nWhen immunized with material which contained the 27 kDa enzyme as \na major constituent, and which was secreted by \n<jats:italic>S. mansoni<\/jats:italic> cercariae during transformation, only 5 of 16 mice \nproduced antibody to this antigen that was detectable in \nWestern blots. The 5 antibody \u2018responder\u2019 mice were significantly \n(<jats:italic>P<\/jats:italic>&lt;0\u00b7001) protected against challenge with a \npercutaneous infection of <jats:italic>S. mansoni<\/jats:italic> cercariae compared with \na group of mice also immunized with CTF, but which had \nnot produced antibodies against the 27\/28 kDa doublet. The results \nindicate that the 27 kDa serine protease of <jats:italic>S. mansoni<\/jats:italic> \nlarvae is a target that is sensitive to immunological attack.<\/jats:p>","DOI":"10.1017\/s0031182097001303","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:36:03Z","timestamp":1027776963000},"page":"237-247","source":"Crossref","is-referenced-by-count":24,"title":["<i>Schistosoma mansoni<\/i>: anomalous immunogenic properties \nof a 27 kDa larval serine protease associated with protective immunity"],"prefix":"10.1017","volume":"115","author":[{"given":"H. Y.","family":"DARANI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. H. C.","family":"CURTIS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"McNEICE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. P.","family":"PRICE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"SAYERS","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"DOENHOFF","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,9,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097001303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T20:22:34Z","timestamp":1557606154000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097001303\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["S0031182097001303"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097001303","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}