{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T15:50:15Z","timestamp":1772812215435,"version":"3.50.1"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[1997,10,1]],"date-time":"1997-10-01T00:00:00Z","timestamp":875664000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>In this paper the <jats:italic>Leishmania<\/jats:italic> (<jats:italic>Viannia<\/jats:italic>) \n<jats:italic>braziliensis<\/jats:italic> complex is defined as containing all \nspecies of the actual subgenus \n<jats:italic>Viannia<\/jats:italic>. Organisms of the <jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>) \n<jats:italic>braziliensis<\/jats:italic> complex are the causative agents of localized human \ncutaneous and \nmucocutaneous leishmaniasis in South America, much of Central America and \nsome areas of North America. In our search \nfor better species and subspecies diagnostic probes we focused our \nresearch on repetitive DNA, since it provides a greater \nnumber of target sites for hybridization. In this work we report the \nisolation and sequencing of a 1\u00b78 kb DNA region, \nLbJ38, which is probably tandemly repeated or dispersed at least 4 times \nalong one chromosome and is naturally present \nin <jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> genomic DNA. This \nregion contains microsatellites and simple repeat DNA sequences and was \nisolated by screening a genomic DNA cosmid library with complex- and \nspecies-specific probes. No homology was found \nwith other <jats:italic>Leishmania<\/jats:italic> microsatellite or repetitive DNA. The \nutility of this repetitive sequence and primers derived from \nit in the identification of <jats:italic>L<\/jats:italic>. (<jats:italic>V<\/jats:italic>) <jats:italic>braziliensis<\/jats:italic> \nis demonstrated. As far as we are aware, this is the first report of sequence \ncharacterized repetitive microsatellite and GC rich simple repeat DNA \nfrom the nuclear genome of New World <jats:italic>Leishmania<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s003118209700139x","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"349-358","source":"Crossref","is-referenced-by-count":20,"title":["Genomic DNA repeat from <i>Leishmania<\/i> (<i>Viannia<\/i>) \n<i>braziliensis<\/i> (Venezuelan strain) containing simple repeats \nand microsatellites"],"prefix":"10.1017","volume":"115","author":[{"given":"N.","family":"RODRIGUEZ","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"DE LIMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"RODRIGUEZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"BREWSTER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. C.","family":"BARKER","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1997,10,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118209700139X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,11]],"date-time":"2019-05-11T16:11:43Z","timestamp":1557591103000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118209700139X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,10]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1997,10]]}},"alternative-id":["S003118209700139X"],"URL":"https:\/\/doi.org\/10.1017\/s003118209700139x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,10]]}}}