{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:05:17Z","timestamp":1764936317449},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"1","license":[{"start":{"date-parts":[[1998,1,1]],"date-time":"1998-01-01T00:00:00Z","timestamp":883612800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,1]]},"abstract":"<jats:p>We have used a styryl dye FM 1\u201343 that cannot cross \nmembranes passively to evaluate membrane vesicle internalization \nprocesses from the heptalaminate surface membrane in schistosomula and \nadult <jats:italic>Schistosoma mansoni<\/jats:italic>. The process of dye \ninternalization was faster in adult worms than in 3 h skin or \n24-h-old mechanically transformed schistosomula. Dye \ninternalization and vesicle formation were inhibited at low \ntemperature which suggests that the process is energy \ndependent. Dye internalization was observed to be a result of \nvesicle formation. This process is impaired by primaquine \nindependently of the parasite stage. The specific Ca<jats:sup>2+<\/jats:sup> \nchelator EGTA affected dye internalization at a concentration of \n5 mm. When parasites were labelled in the presence of genistein \n(a tyrosine kinase inhibitor) uptake of the dye was blocked \nin 24-h-old schistosomula. However, only a partial blockage of the \ndye internalization was seen in adult worms. These \nresults suggest that dye internalization mediated by tyrosine \nkinase signalling is developmentally regulated.<\/jats:p>","DOI":"10.1017\/s0031182097001960","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"51-59","source":"Crossref","is-referenced-by-count":16,"title":["Membrane internalization processes in different stages \nof <i>Schistosoma mansoni<\/i> as shown by a styryl dye \n(Frei Mao 1\u201343)"],"prefix":"10.1017","volume":"116","author":[{"given":"F.","family":"RIBEIRO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M. Z.","family":"COELHO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Q.","family":"VIEIRA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"POWELL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. R.","family":"KUSEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,1,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097001960","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,3]],"date-time":"2019-04-03T18:58:41Z","timestamp":1554317921000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097001960\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,1]]}},"alternative-id":["S0031182097001960"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097001960","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,1]]}}}