{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T10:34:28Z","timestamp":1774434868661,"version":"3.50.1"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p>The Zn<jats:sup>2+<\/jats:sup>-chelating metalloprotease inhibitor 1,10-phenanthroline\n \n(phenanthroline, 5\u2013150 \u03bc<jats:sc>M<\/jats:sc>) elicited dose-dependent \ncontraction of the longitudinal and circular (transverse) musculature of\n adult \nmale schistosomes. At the same concentrations, phenanthroline did not cause\n \ncontraction of dispersed individual muscle fibres. The phenanthroline-induced\n \ncontractions were reduced by the inclusion of 100 or 300 \u03bc<jats:sc>M<\/jats:sc>\n Zn<jats:sup>2+<\/jats:sup> \nin the extracellular medium. Phenanthroline \n(0\u00b75\u2013150 \u03bc<jats:sc>M<\/jats:sc>) also inhibited the egg production\n of \nadult worm pairs <jats:italic>in vitro<\/jats:italic>, with a 98% reduction at 50\n\u03bc<jats:sc>M<\/jats:sc>.\n When worm \npairs were exposed to phenanthroline, the males detached from the dish\n \nand released the females, resulting in unpaired \nworms. At the higher concentrations (50 and 150 \u03bc<jats:sc>M<\/jats:sc>), the worms\n \nwere killed <jats:italic>in vitro<\/jats:italic>. Worm burdens were reduced by \nover 50% in infected mice injected with phenanthroline (20 mg\/kg\/day\n \nfor 4 days), but twice the dose resulted in only \na 25% reduction. Phenanthroline injections also induced an hepatic shift\n \nand an unpairing of adult worms in infected \nmice, and the female worms appeared degenerate and lacked gut pigmentation.\n \nMice fed a diet containing 0\u00b73% \nphenanthroline received significant protection from infection when challenged\n \nwith schistosome cercaria, where \nphenanthroline-fed mice had 94% fewer adult worms than control mice. The\n \nbroad range of phenanthroline effects on \nschistosomes suggests broad and important functions for metalloproteases\n in these worms.<\/jats:p>","DOI":"10.1017\/s0031182097002370","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"319-325","source":"Crossref","is-referenced-by-count":24,"title":["The metalloprotease inhibitor 1,10-phenanthroline affects \n<i>Schistosoma mansoni<\/i> motor activity, egg laying and viability"],"prefix":"10.1017","volume":"116","author":[{"given":"T. A.","family":"DAY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.-Z.","family":"CHEN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,4,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182097002370","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T18:59:49Z","timestamp":1554577189000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182097002370\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["S0031182097002370"],"URL":"https:\/\/doi.org\/10.1017\/s0031182097002370","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4]]}}}