{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:54:38Z","timestamp":1792148078288,"version":"4.4.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[1998,4,1]],"date-time":"1998-04-01T00:00:00Z","timestamp":891388800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,4]]},"abstract":"<jats:p><jats:italic>Echinococcus multilocularis<\/jats:italic> is a cestode parasite that\n \npredominantly infects red and arctic foxes as definitive hosts. Ingestion\n \nof <jats:italic>E. multilocularis<\/jats:italic> eggs and subsequent post-oncospheral \ninfection with the larval stage (metacestode) of the parasite \nresults in alveolar echinococcosis (AE), a life-threatening hepatic \ndisease concerning humans and other intermediate hosts \nsuch as small rodents. The primary fluid-filled vesicles of the \nasexually proliferating metacestode are comprised of an inner \ngerminal layer, a syncytial tegument, and an outer, acellular, so-called\n \nlaminated layer. This laminated layer may play an \nimportant role in protecting the developing <jats:italic>E. multilocularis<\/jats:italic>\n \nmetacestode from host immune reactions, and laminated \nlayer-associated components represent potential targets for intervention\n \nduring the course of AE. We have used an <jats:italic>in vitro<\/jats:italic> \ncultivation technique for the long-term maintenance and proliferation of\n \n<jats:italic>E. multilocularis<\/jats:italic> metacestodes in order to generate \npremature (protoscolex-free) parasite vesicles. A polyclonal antiserum\n \nwas raised against this host-free parasite tissue. \nSubsequent immunoblot analysis of parasite fractions obtained by Triton\n \nX-114 extraction led to the identification of a \n116 kDa protein (named EmP2) within the Triton-insoluble fraction. The\n \ncharacterization of EmP2 by SDS\u2013PAGE, \nWestern blotting, and by immunofluorescence revealed that EmP2 is a \nlaminated layer-associated protein.<\/jats:p>","DOI":"10.1017\/s0031182098002406","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"363-372","source":"Crossref","is-referenced-by-count":20,"title":["Identification of a laminated layer-associated protein in \n<i>Echinococcus multilocularis<\/i> metacestodes"],"prefix":"10.1017","volume":"116","author":[{"given":"K.","family":"INGOLD","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"GOTTSTEIN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"HEMPHILL","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1998,4,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098002406","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T18:59:53Z","timestamp":1554577193000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098002406\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,4]]}},"alternative-id":["S0031182098002406"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098002406","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4]]}}}