{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T08:50:15Z","timestamp":1762159815752},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"5","license":[{"start":{"date-parts":[[1998,5,1]],"date-time":"1998-05-01T00:00:00Z","timestamp":893980800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p>The kinetics of serum antibody response against infection with\n \n<jats:italic>Echinococcus multilocularis<\/jats:italic> eggs was evaluated in AKR \nmice. The animals were infected by oral inoculation with 300 parasite eggs,\n \nand necropsied at 1, 2, 4, 6, 9, 12 and 16 weeks \npost-infection (p.i.), respectively. The parasite formed the laminated\n layer \nat 4 weeks p.i., the brood capsule with a massive \nproliferation of germinal cells at 9 weeks p.i. and protoscoleces at \n16 weeks p.i. Serum antibody responses of the mice to \nantigen preparations from metacestodes of different stages and protoscoleces\n \nwere evaluated by ELISA, immunoblotting \nand immunohistochemistry. In ELISA, the antibody responses began to \nincrease at 4 weeks and became more apparent \nat 9 weeks p.i. and thereafter. Immunoblots using sera collected at \n16 weeks p.i. showed some common bands among the \n3 different antigen preparations. In addition to this, the germinal cells\n \nand brood capsules of mature metacestodes were \nstained strongly in an immunohistochemical study. From above, it is suggested\n \nthat some antigen molecules are expressed \nin the parasite through these stages and stimulated host antibody responses.<\/jats:p>","DOI":"10.1017\/s0031182098002443","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"463-469","source":"Crossref","is-referenced-by-count":9,"title":["Time-course of antibody response in mice against oral \ninfection with eggs of <i>Echinococcus multilocularis<\/i>"],"prefix":"10.1017","volume":"116","author":[{"given":"J.","family":"MATSUMOTO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"YAGI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"NONAKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"OKU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"KAMIYA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,5,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098002443","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T15:32:34Z","timestamp":1554564754000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098002443\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1998,5]]}},"alternative-id":["S0031182098002443"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098002443","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,5]]}}}