{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T10:30:42Z","timestamp":1694514642449},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"4","license":[{"start":{"date-parts":[[1998,10,1]],"date-time":"1998-10-01T00:00:00Z","timestamp":907200000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,10]]},"abstract":"<jats:p>Schistosomula of <jats:italic>Schistosoma mansoni<\/jats:italic> were incubated in\n \nRPMI 1640 medium containing 10% fetal calf serum, 10% \nhuman portal venous or 10% human peripheral venous sera in the \npresence of bromodeoxyuridine (BrdU) in order to \nmeasure differences in cell proliferation. The rates of cell proliferation\n \nas expressed by BrdU labelling indices (BLI) were \ndetermined as a function of time of incubation by immunohistochemistry\n \nusing monoclonal antibody to BrdU. Compared \nto schistosomula cultured in the presence of RPMI plus 10% fetal calf \nserum, BLIs were increased by 60% in the presence \nof human portal, but not in peripheral serum. This stimulatory effect \nwas substantially reproduced by a fraction of portal \nserum with a molecular weight range between 1 and 50 kDa. However, \nin the presence of human peripheral venous serum, \neither whole or fractionated, schistosomula showed no significant \ndifference compared to RPMI plus 10% fetal calf serum \nalone. Furthermore, human portal serum fractions of molecular weight \ngreater than 50 kDa also revealed no significant \ndifference relative to control. The results indicate that portal \nvenous serum component(s) of a molecular weight range \nhigher than most simple nutrients can greatly stimulate the rate \nof cell proliferation of <jats:italic>Schistosoma mansoni<\/jats:italic> schistosomula.<\/jats:p>","DOI":"10.1017\/s0031182098003096","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"293-299","source":"Crossref","is-referenced-by-count":8,"title":["Human portal serum stimulates cell proliferation in \nimmature <i>Schistosoma mansoni<\/i>"],"prefix":"10.1017","volume":"117","author":[{"given":"Y. M.","family":"SHAKER","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. H.","family":"WU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. A.","family":"EL-SHOBAKI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"ASHOUR","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M.","family":"KHATTAB","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M.","family":"DRAZ","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"KAMEL","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. Y.","family":"WU","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1998,10,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098003096","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T20:07:31Z","timestamp":1557518851000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098003096\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,10]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1998,10]]}},"alternative-id":["S0031182098003096"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098003096","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,10]]}}}