{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:39:47Z","timestamp":1790509187489,"version":"4.1.0"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"6","license":[{"start":{"date-parts":[[1998,12,1]],"date-time":"1998-12-01T00:00:00Z","timestamp":912470400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1998,12]]},"abstract":"<jats:p>Severe cryptosporidial infections were produced in gamma interferon\n (IFN-\u03b3) knockout mice. Mean oocyst shedding \nincreased from 332 to 30717 oocysts\/100 \u03bcl of faecal suspension\n between day 4 and 9 after administration of 1\u00d710<jats:sup>5<\/jats:sup>\noocysts\/mouse. No significant differences in oocyst shedding were observed\n in mice after being inoculated with 1\u00d710<jats:sup>5<\/jats:sup>, 1\u00d710<jats:sup>4<\/jats:sup>\n or \n1\u00d710<jats:sup>3<\/jats:sup> \noocysts\/mouse (<jats:italic>P<\/jats:italic>&gt;0\u00b705). Infected mouse weights decreased\n an average 3\u20134 g before death or \neuthanization. Histological studies revealed heavy parasite colonization\n in small intestinal epithelium (approximately \n250 organisms\/high-power field at \u00d7400). Mesenteric lymph nodes\n in infected mice were markedly enlarged compared \nto controls (<jats:italic>P<\/jats:italic>&lt;0\u00b705). Both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup>\n \nT cell populations increased in spleens of infected mice while the B cell\n \npopulation increased in mesenteric lymph nodes from infected mice. No significant\n proliferation was observed when \npooled lymphocytes from infected mice were exposed to <jats:italic>C. parvum<\/jats:italic>\n antigens <jats:italic>in vitro<\/jats:italic>. Addition of recombinant mouse \nIFN-\u03b3 did not restore antigen responsiveness. While lymphoproliferative\n responses to specific antigen were not significant \nin the short period following infection, this mouse model provides unique\n features to study the characteristics of acute \ninfection and the immune response against <jats:italic>C. parvum<\/jats:italic>.<\/jats:p>","DOI":"10.1017\/s0031182098003424","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"525-531","source":"Crossref","is-referenced-by-count":37,"title":["Characterization of experimental <i>Cryptosporidium parvum<\/i> \ninfection in IFN-\u03b3 knockout mice"],"prefix":"10.1017","volume":"117","author":[{"given":"XIANGDONG","family":"YOU","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. R.","family":"MEAD","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1998,12,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098003424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,10]],"date-time":"2019-05-10T15:48:57Z","timestamp":1557503337000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098003424\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1998,12]]}},"alternative-id":["S0031182098003424"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098003424","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,12]]}}}