{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T06:45:05Z","timestamp":1789454705628,"version":"build-2803163510"},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"2","license":[{"start":{"date-parts":[[1999,2,1]],"date-time":"1999-02-01T00:00:00Z","timestamp":917827200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,2]]},"abstract":"<jats:p>In the present study, polymerase chain reaction\u2013restriction\n fragment length polymorphism (PCR\u2013RFLP) analysis was \ndeveloped to identify 5 species (<jats:italic>Trichinella spiralis<\/jats:italic>, <jats:italic>Trichinella\n britovi<\/jats:italic>, <jats:italic>Trichinella nativa<\/jats:italic>, <jats:italic>Trichinella nelsoni<\/jats:italic>\n and <jats:italic>Trichinella \npseudospiralis<\/jats:italic>) and 3 phenotypes of uncertain taxonomic status (<jats:italic>Trichinella<\/jats:italic>\n T5, T6, and T8). Eleven restriction endonucleases \nwere used to restrict 3 DNA fragments (1) a 2800 bp fragment of the 43\n kDa excretory\u2013secretory (E\u2013S) protein \ngene, (2) a 1250 bp fragment amplified with the primer pair SB147A and\n (3) a 372 bp fragment amplified with the primer \npair SB372A. This RFLP method allows the identification of the 8 <jats:italic>Trichinella<\/jats:italic>\n phenotypes as follows: <jats:italic>T. spiralis<\/jats:italic> by the \n<jats:italic>Hinf<\/jats:italic>I or <jats:italic>Dde<\/jats:italic>I endonuclease restriction of the 2800 bp\n fragment; <jats:italic>T. nativa<\/jats:italic> by the <jats:italic>Rsa<\/jats:italic>I restriction of the 2800\n bp fragment, \nor by the <jats:italic>Alu<\/jats:italic>I restriction of the 1250 bp fragment; <jats:italic>T. britovi<\/jats:italic>\n and <jats:italic>Trichinella<\/jats:italic> T8 by the <jats:italic>Alu<\/jats:italic>I restriction of the 1250\n bp \nfragments, and can be discriminated between them by the <jats:italic>Ssp<\/jats:italic>I restriction\n of the 2800 bp fragment; <jats:italic>T. pseudospiralis<\/jats:italic> by \nthe <jats:italic>Msp<\/jats:italic>I restriction of the 372 bp fragment; <jats:italic>T. nelsoni<\/jats:italic>\n by the <jats:italic>Hha<\/jats:italic>I or <jats:italic>Alu<\/jats:italic>I restriction of the 2800 bp fragment;\n <jats:italic>Trichinella<\/jats:italic> \nT5 by the <jats:italic>Hha<\/jats:italic>I restriction of the 2800 bp fragment; <jats:italic>Trichinella<\/jats:italic>\n T6 by the <jats:italic>Alu<\/jats:italic>I restriction of the 1250 bp fragment; and \n<jats:italic>Trichinella<\/jats:italic> T8 by the <jats:italic>Ssp<\/jats:italic>I or <jats:italic>Rsa<\/jats:italic>I restriction\n of the 2800 bp fragment. This study reveals also an intraspecifies polymorphism\n \nin the 2800 bp and 1250 bp fragments for <jats:italic>T. britovi<\/jats:italic>, <jats:italic>Trichinella<\/jats:italic>\n T5 and T6.<\/jats:p>","DOI":"10.1017\/s0031182098003679","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:30:22Z","timestamp":1027762222000},"page":"211-218","source":"Crossref","is-referenced-by-count":45,"title":["Polymerase chain reaction\u2013restriction fragment length \npolymorphism (PCR\u2013RFLP) for the identification of \n<i>Trichinella<\/i> isolates"],"prefix":"10.1017","volume":"118","author":[{"given":"Z.","family":"WU","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"NAGANO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"POZIO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"TAKAHASHI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"56","published-online":{"date-parts":[[1999,2,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098003679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T15:48:10Z","timestamp":1554133690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098003679\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,2]]}},"alternative-id":["S0031182098003679"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098003679","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,2]]}}}