{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:50:40Z","timestamp":1760266240249},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"3","license":[{"start":{"date-parts":[[1999,3,1]],"date-time":"1999-03-01T00:00:00Z","timestamp":920246400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,3]]},"abstract":"<jats:p>Phosphorylcholine (PC) is a common constituent of proteins secreted\n by filarial nematodes. As this substance has been \nshown to interfere with immune responses, we are interested in designing\n strategies for blocking its attachment. Towards \nthis end, we are investigating the mechanism of incorporation of PC into\n filarial molecules and in the present manuscript \nwe describe experiments relating to elucidating the source of PC for attachment.\n Synthesis of phosphatidylcholine in \neukaryotic organisms can occur by a mechanism involving the transfer of\n PC from CDP-choline to diacylglycerol (the \nKennedy pathway). By (i) measuring transfer of radio-isotope labelled PC\n from CDP-choline to parasite molecules and \n(ii) employing inhibitors of CDP-choline synthesis, we have investigated\n whether CDP-choline can act as a source of PC \nfor transfer to ES\u201362, a major secreted glycoprotein of the rodent\n filarial nematode <jats:italic>Acanthocheilonema viteae<\/jats:italic>. Although we \ncan find no evidence of this, we show that attachment of PC is blocked\n by hemicholinium-3, an inhibitor of choline kinase, \nthe first enzyme in the Kennedy pathway. Thus, at least the first step\n in this pathway \u2013 phosphorylation of choline, would \nappear to be necessary for attachment of PC to ES-62.<\/jats:p>","DOI":"10.1017\/s0031182098003722","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:30:22Z","timestamp":1027776622000},"page":"311-318","source":"Crossref","is-referenced-by-count":16,"title":["Mechanisms underlying the transfer of phosphorylcholine \nto filarial nematode glycoproteins \u2013 a possible role for \ncholine kinase"],"prefix":"10.1017","volume":"118","author":[{"given":"K. M.","family":"HOUSTON","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"HARNETT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,3,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182098003722","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T19:18:43Z","timestamp":1554146323000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182098003722\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1999,3]]}},"alternative-id":["S0031182098003722"],"URL":"https:\/\/doi.org\/10.1017\/s0031182098003722","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,3]]}}}