{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T19:20:55Z","timestamp":1698520855395},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[1999,11,1]],"date-time":"1999-11-01T00:00:00Z","timestamp":941414400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:p>The filarial nematodes <jats:italic>Wuchereria bancrofti<\/jats:italic>, <jats:italic>Brugia malayi<\/jats:italic> and <jats:italic>Onchocerca volvulus<\/jats:italic> represent major public health \nproblems in the Tropics. Effective diagnosis of infection with these parasites is required both for administration of drugs \nto infected individuals and for monitoring of control programs. However parasitological diagnosis is associated with a \nnumber of problems including frequently inadequate sensitivity, long pre-patency of infection and inconvenience for \npatients. For these reasons there has been considerable effort expended in developing other forms of diagnosis, in particular \nimmunoassays for measuring antibody and circulating parasite antigen as well as molecular-biology-based assays for \ndetecting parasite DNA. This article reviews the progress and achievements obtained to date. The latter include the \ndevelopment of ELISAs employing recombinant antigen for detection of antibody to <jats:italic>O. volvulus<\/jats:italic> which have both high \nsensitivity and specificity, the commercial availability of immunoassays to measure circulating antigen in <jats:italic>W. bancrofti<\/jats:italic> \ninfection and the generation of specific DNA-based detection systems for all three parasites.<\/jats:p>","DOI":"10.1017\/s0031182099004084","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T13:36:03Z","timestamp":1027776963000},"page":"59-71","source":"Crossref","is-referenced-by-count":17,"title":["Molecular and immunodiagnosis of human filarial nematode infections"],"prefix":"10.1017","volume":"117","author":[{"given":"W.","family":"HARNETT","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"BRADLEY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"GARATE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,11,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S0031182099004084","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T20:04:39Z","timestamp":1557432279000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S0031182099004084\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["S0031182099004084"],"URL":"https:\/\/doi.org\/10.1017\/s0031182099004084","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]}}}