{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T17:30:04Z","timestamp":1703439004905},"reference-count":0,"publisher":"Cambridge University Press (CUP)","issue":"7","license":[{"start":{"date-parts":[[1999,10,1]],"date-time":"1999-10-01T00:00:00Z","timestamp":938736000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/www.cambridge.org\/core\/terms"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasitology"],"published-print":{"date-parts":[[1999,10]]},"abstract":"<jats:p>The phylum Apixomplexa includes obligate intracellular parasites that are of enormous medical and veterinary significance, \nas they are responsible for a wide variety of diseases including malaria, toxoplasmosis, coccidiosis, cryptosporidiosis, \ntheileriosis and babesiosis. The EST sequencing projects in <jats:italic>Toxoplasma gondii<\/jats:italic> and the <jats:italic>Plasmodium falciparum<\/jats:italic> genome \nsequencing project have greatly accelerated gene discovery, revealing for example novel coding sequences restricted to the \nApicomplexa. However, easy acquisition of sequence is almost useless if the function of any given gene cannot be tested. \nThe establishment of transfection systems in <jats:italic>Toxoplasma gondii<\/jats:italic>, <jats:italic>Neospora<\/jats:italic> and in several <jats:italic>Plasmodium<\/jats:italic> species has provided \nus with the reverse genetics methods appropriate to the functional analysis of genes. Over the past few years, the discovery \nof novel genes coupled to the ability to introduce or modify genes has already contributed to a better understanding of \ncell biology and pathogenesis of these obligate intracellular parasites. Some insights into the complex processes of parasite \ninvasion, differentiation, regulation of gene expression and protein trafficking are emerging although identification of the \nexact functional roles for many molecules is still awaiting more investigation. This review summarizes progress in this \narea. It also emphasises the tight link and synergy between <jats:italic>Toxoplasma<\/jats:italic> and malaria research. The use of reverse genetics \ndoes not guarantee the answer to gene function, so we can learn from both failed and successful experiments about how \nbetter and more efficiently to use \u2018genomics\u2019 to accelerate discoveries relevant to the understanding of parasitism by \nApicomplexa.<\/jats:p>","DOI":"10.1017\/s003118209900414x","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:36:03Z","timestamp":1027762563000},"page":"53-61","source":"Crossref","is-referenced-by-count":13,"title":["The importance of reverse genetics in determining gene \nfunction in apicomplexan parasites"],"prefix":"10.1017","volume":"118","author":[{"given":"M.","family":"SOETE","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"HETTMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"SOLDATI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"56","published-online":{"date-parts":[[1999,10,1]]},"container-title":["Parasitology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.cambridge.org\/core\/services\/aop-cambridge-core\/content\/view\/S003118209900414X","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,5,9]],"date-time":"2019-05-09T16:09:33Z","timestamp":1557418173000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.cambridge.org\/core\/product\/identifier\/S003118209900414X\/type\/journal_article"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,10]]},"references-count":0,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1999,10]]}},"alternative-id":["S003118209900414X"],"URL":"https:\/\/doi.org\/10.1017\/s003118209900414x","relation":{},"ISSN":["0031-1820","1469-8161"],"issn-type":[{"value":"0031-1820","type":"print"},{"value":"1469-8161","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,10]]}}}